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<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Hi Everyone,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">We are happy to announce the Spring 2023 Tomography and Cryo-FIB Workshop at the Midwest Center for Cryo-ET at UW-Madison. The workshop will be held from April 17-20, 2023. In
addition to our MCCET team, we have an exciting group of confirmed external speakers/trainers that includes Alberto Bartesaghi (Duke), Daija Bobe (NCITU), Alex Hall (TFS), Zunlong Ke (MPI), Lauren Ann Metskas (Purdue), Marisa Otegui (UW-Madison), Sergey Suslov
(TFS), and Veronika Vrbovska (TFS).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:black;background:white">The morning theory sessions of the workshop will be hybrid and the afternoon practical sessions will be in-person only.<span class="apple-converted-space"> <o:p></o:p></span></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal" style="line-height:13.5pt"><b><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:black">Training topics will include:</span></b><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:black"> <o:p></o:p></span></p>
<ul type="disc">
<li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;margin-left:-.25in;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-size:12.0pt;font-family:"Arial",sans-serif">Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters. <o:p></o:p></span></li><li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;margin-left:-.25in;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-size:12.0pt;font-family:"Arial",sans-serif">Training in data acquisition workflows that include screening, data collection, flexible scripting, and basic data processing. <o:p></o:p></span></li><li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;margin-left:-.25in;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-size:12.0pt;font-family:"Arial",sans-serif">Hands-on skills development with specimen preparation methods including PRIMO micro-patterning, plunge freezing, high-pressure freezing, and other novel approaches. <o:p></o:p></span></li><li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;margin-left:-.25in;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-size:12.0pt;font-family:"Arial",sans-serif">Instruction in 2D/3D correlative imaging workflows using a cryo-confocal microscope and the integrated fluorescence module in the Aquilos 2. <o:p></o:p></span></li><li class="MsoNormal" style="color:black;mso-margin-top-alt:auto;mso-margin-bottom-alt:auto;margin-left:-.25in;line-height:13.5pt;mso-list:l0 level1 lfo1">
<span style="font-size:12.0pt;font-family:"Arial",sans-serif">Guidance in FIB-milling with AutoTEM and advanced FIB workflows such as waffle milling and lift-out methods. <o:p></o:p></span></li></ul>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Please register by using this link:
<a href="https://urldefense.com/v3/__https://explore.wisc.edu/LP=1397__;!!Mih3wA!HAc5ilc-3-5wbDWHECfX1npSTxsVCgDHCjl5obA3dizBUkVR6HMgvbBFINNh-ctpt8Ihr6VtjqDmR22WGk3vFmE$">https://explore.wisc.edu/LP=1397</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Registration closes on March 3, 2023.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">If you have any questions, please reach us at
<a href="mailto:cryoem@biochem.wisc.edu">cryoem@biochem.wisc.edu</a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Best regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif">Liz<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
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<p class="MsoNormal">----<o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Elizabeth R. Wright, PhD</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Professor, Department of Biochemistry</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Cryo-Electron Microscopy Research Center</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Midwest Center for Cryo-Electron Tomography</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">University of Wisconsin-Madison</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Morgridge Institute for Research</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black"><a href="mailto:erwright2@wisc.edu" title="mailto:erwright2@wisc.edu"><span style="color:#0086F0">erwright2@wisc.edu</span></a></span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black"> </span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black">Assistant: Alex Cotten</span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black"><a href="mailto:cotten@wisc.edu" title="mailto:cotten@wisc.edu"><span style="color:#0086F0">cotten@wisc.edu</span></a></span><span style="color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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