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Hi Ed,
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</div>
<div class="">We have Pelco EasiGlow, a Quorum GloQube, a Tergeo PIE scientific plasma cleaner and an old Cressington 208 unit in Leeds. </div>
<div class=""><br class="">
</div>
<div class="">Bottom line is I really really like the GloQube for the dual chamber for glow discharge in air/vapour (we have several users who get good use of the second chamber for amylamine). We have it on a scroll pump so they cycle length is a bit long
but its workable. This is the most commonly used system for our cryoEM users. </div>
<div class=""><br class="">
</div>
<div class="">The Pelco units are mostly used for negative stain here, I found them personally to be a little unpredictable for cryoEM but this could be a system specific thing (or a me thing..). Cheap and cheerful. Users are forever chipping the glass but
you can repair with resin easily enough. </div>
<div class=""><br class="">
</div>
<div class="">We tested the Tergeo PIE system and Gatan Solarus when we purchased a system to work in defined gas mixes. The tergeo PIE performed well in testing in our hands (as well/better than the Solarus II), and subsequently, so we are very happy with
it for cryoEM applications. And of course it is much cheaper. Also gets some use from our friends in material sciences. Worst thing about it is the name...</div>
<div class=""><br class="">
</div>
<div class="">Despite the options available, for my own personal single particle grids I would still tend to use use the Cresssington 208 (or the GloQube). </div>
<div class=""><br class="">
</div>
<div class="">Happy to share quotes/recipes if this is helpful for anyone. </div>
<div class=""><br class="">
<div class="">
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<div>Best wishes<br class="">
Becky</div>
<div>______________________________________________________________<br class="">
<br class="">
Rebecca Thompson (She/her)<br class="">
Head of Faculty Biological Sciences Research Facilities<br class="">
Deputy Director, Astbury Biostructure <br class="">
Laboratory Electron Microscopy <br class="">
<br class="">
University of Leeds<br class="">
Mobile (+44) 07816179813<br class="">
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<blockquote type="cite" class="">
<div class="">On 25 Jan 2023, at 17:51, Patricia Grob PhD <<a href="mailto:pgrob@berkeley.edu" class="">pgrob@berkeley.edu</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
Hello Ed,
<div class=""><br class="">
</div>
<div class="">We got a Tergeo EM (PIE Scientific) plasma cleaner when our Solarus went down 4 or 5 years ago and we have been very happy with it. It is more affordable and it has been running without fail or repairs ever since.</div>
<div class=""><br class="">
</div>
<div class="">Patricia</div>
<div class=""><br class="">
<div class="">
<div class="">_________________________________</div>
<div class="">Patricia Grob, Ph.D.<br class="">
Research Specialist<br class="">
Nogales Lab<br class="">
HHMI at UC Berkeley<br class="">
Molecular & Cell Biology Department / QB3,<br class="">
Stanley Hall #742<br class="">
University of California, Berkeley<br class="">
Berkeley, CA 94720-3220<br class="">
Phone: (510)666-3335<span class="Apple-tab-span" style="white-space: pre;"> </span>
Fax: (510)666-3336<br class="">
E.M. suite B307: (510)666-3339<br class="">
URL: <a href="https://urldefense.com/v3/__https://eur03.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__http*3A*2F*2Fcryoem.berkeley.edu__*3B!!Mih3wA!C7DcZr7wzSTHKwpZuUfXknBo_VK608jMkHM0LJNxPRO0sFyRNtyFeiQhOVGG_ZYiKbC1QD2tjWmplJUuHY_C*24&data=05*7C01*7Cr.f.thompson*40leeds.ac.uk*7Cfb8a26703d2641a2a40108dafefce127*7Cbdeaeda8c81d45ce863e5232a535b7cb*7C1*7C0*7C638102659351038257*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=igMiQOAHj8gdMfZtQJ4B8M8L0JC4ZFrCCgpYrvWofQU*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!CFnxHo6kA-dBhVub3tppy3SSoGNGGwgLT3eDQkQYRPSJeiAhl9i49BsGYc1Hd3ybiMmc8hRkxhY3FPGiz4267wtYKFvE$" originalsrc="https://urldefense.com/v3/__http://cryoem.berkeley.edu__;!!Mih3wA!C7DcZr7wzSTHKwpZuUfXknBo_VK608jMkHM0LJNxPRO0sFyRNtyFeiQhOVGG_ZYiKbC1QD2tjWmplJUuHY_C$" shash="rFLTruxj6iiEYwk6xgQ4/dJ0ctMU//IhsmodU2Ix/HzQsqcRKBBUW94itutc6zKoyvZsLL4OoBp+saLfC8YW7YREe4H09/doD+NU4GX/19RdkwYz8EKJ4ysXxFOWylxjd9Tq1nwebWff3XDBHWEiDa9Qs/luD6oR78Yc9eqQPws=" class="">
cryoem.berkeley.edu</a><br class="">
<a href="mailto:pgrob@berkeley.edu" class="">Email: pgrob@berkeley.edu</a><br class="">
<br class="">
<br class="">
</div>
</div>
<div class=""><br class="">
<blockquote type="cite" class="">
<div class="">On Jan 24, 2023, at 2:43 PM, Christopher Winkler <<a href="mailto:microwink@gmail.com" class="">microwink@gmail.com</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div dir="ltr" class="">Hey Ed,
<div class=""><br class="">
</div>
<div class="">We went through a similar cost benefit analysis of plasma cleaners after our Solarus RF board failed. We went with one of the more basic plasma cleaners partly due to budget, partly due to the simplicity of operation of the more basic units, and
partly due to the simplicity of design of the basic units. As you point out, the cost of a "simple" cleaner is 1/4 to 1/3 of the Solarus II. The Solarus was pretty easy to operate and the ability to save recipes is nice, but the more basic plasma cleaners
are even simpler--you basically toggle a switch to start or stop the cleaner. There is no ability to save a recipe on our cleaner but we only had a few saved recipes on our Solarus that were routinely used. It's not hard to adjust time and power on the basic
units. Most importantly for me, the more basic cleaners forego the TMP for a basic dry scroll pump so there are no diaphragm pumps to rebuild or turbo oil bearings to swap, and there's no painful teardown of the system to access the vacuum systems. The poorer
vacuum is fully sufficient to generate a good quality plasma and I've not really seen much of a difference in cleaning performance--the new "simple" model does a good job of cleaning and removing hydrocarbon contamination (we don't use it for glow discharging
or treating grid surfaces, so I can't comment on that). </div>
<div class=""><br class="">
</div>
<div class="">A downside of the basic cleaner is that it only uses one process gas at a time, so you cannot switch between argon and oxygen mixtures or hydrogen like the Solarus. This is not a problem for our application but it's something to consider. </div>
<div class=""><br class="">
</div>
<div class="">Happy to discuss specifics off of the listserv,</div>
<div class="">Chris</div>
</div>
<br class="">
<div class="gmail_quote">
<div dir="ltr" class="gmail_attr">On Tue, Jan 24, 2023 at 5:17 PM Egelman, Edward H (ehe2n) <<a href="mailto:egelman@virginia.edu" class="">egelman@virginia.edu</a>> wrote:<br class="">
</div>
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<div class="msg7018277512538944471">
<div lang="EN-US" style="overflow-wrap: break-word;" class="">
<div class="m_7018277512538944471WordSection1">
<p class="MsoNormal">We have a Gatan Solarus that is failing, and are thinking about replacements. The newer Solarus II is from 5-10 times more expensive than the simplest plasma cleaners out there. Does anyone have any comparisons to justify this very high
price tag?<u class=""></u><u class=""></u></p>
<p class="MsoNormal">Thanks,<u class=""></u><u class=""></u></p>
<p class="MsoNormal">Ed<u class=""></u><u class=""></u></p>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class=""><u class=""></u> <u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class=""><u class=""></u> <u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class="">Edward Egelman<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class="">Harrison Distinguished Professor,<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class="">Department of Biochemistry and Molecular Genetics<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class="">Professor of Molecular Physiology and Biological Physics</span><span style="font-size:11pt" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size: 10.5pt;" class="">University of Virginia</span><span style="font-size:11pt" class=""><u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class="">phone: 434-924-8210<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class="">fax: 434-924-5069<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-size: 10.5pt;" class=""><a href="mailto:egelman@virginia.edu" target="_blank" class=""><span style="color:blue" class="">egelman@virginia.edu</span></a><u class=""></u><u class=""></u></span></p>
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