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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Segoe UI",sans-serif;background:white">Dear colleagues
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Segoe UI",sans-serif;background:white">Are you looking for a postdoc position (or knows someone who does).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Segoe UI",sans-serif;background:white"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Segoe UI",sans-serif;background:white">Then there is still a bit of time to apply for our open postdoc position based at the Dept. of Plant and Environmental Sciences at Univ. Copenhagen with collaborations
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Segoe UI",sans-serif;background:white">Apply via <a href="https://urldefense.com/v3/__https://employment.ku.dk/faculty/?show=158020__;!!Mih3wA!BLmW-AaYfjHJAxYdqeeuqnwB76eMzTJqZrF4cCF41tY6QgiP6rnysvBnYJlxF_86kn6fGUKYpLJtBhNY0w$">https://employment.ku.dk/faculty/?show=158020</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Segoe UI",sans-serif">Best</span><span style="font-size:10.0pt;font-family:"Verdana",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal" style="line-height:8.0pt"><b><span lang="DA" style="font-size:7.5pt;font-family:"Verdana",sans-serif;color:gray">Københavns Universitet<o:p></o:p></span></b></p>
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<p class="MsoNormal" style="line-height:8.0pt"><span style="font-size:7.5pt;font-family:"Verdana",sans-serif;color:gray;mso-fareast-language:DA">Section for Plant Glycobiology<o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:8.0pt"><span style="font-size:7.5pt;font-family:"Verdana",sans-serif;color:gray;mso-fareast-language:DA">Thorvaldsensvej 40<o:p></o:p></span></p>
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<p class="MsoNormal" style="mso-line-height-alt:8.0pt"><a href="https://urldefense.com/v3/__https://informationssikkerhed.ku.dk/persondatabeskyttelse/privatlivspolitik/__;!!Mih3wA!BLmW-AaYfjHJAxYdqeeuqnwB76eMzTJqZrF4cCF41tY6QgiP6rnysvBnYJlxF_86kn6fGUKYpLLrY6Av8g$"><u><span lang="DA" style="font-size:7.5pt;font-family:"Verdana",sans-serif;color:gray;mso-fareast-language:DA;text-decoration:none">Sådan
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>Patricia Grob PhD<br>
<b>Sent:</b> 25. januar 2023 18:52<br>
<b>To:</b> Egelman, Edward H (ehe2n) <egelman@virginia.edu><br>
<b>Cc:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] plasma cleaner<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<span style="font-size:9.0pt;font-family:wf_segoe-ui_normal;color:#212121">You don't often get email from
</span><a href="mailto:pgrob@berkeley.edu"><span style="font-size:9.0pt;font-family:wf_segoe-ui_normal">pgrob@berkeley.edu</span></a><span style="font-size:9.0pt;font-family:wf_segoe-ui_normal;color:#212121">.
</span><a href="https://urldefense.com/v3/__https://aka.ms/LearnAboutSenderIdentification__;!!Mih3wA!BLmW-AaYfjHJAxYdqeeuqnwB76eMzTJqZrF4cCF41tY6QgiP6rnysvBnYJlxF_86kn6fGUKYpLLd5EmuRg$"><span style="font-size:9.0pt;font-family:wf_segoe-ui_normal">Learn why this is important</span></a><span style="font-size:9.0pt;font-family:wf_segoe-ui_normal;color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal">Hello Ed, <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">We got a Tergeo EM (PIE Scientific) plasma cleaner when our Solarus went down 4 or 5 years ago and we have been very happy with it. It is more affordable and it has been running without fail or repairs ever since.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Patricia<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">_________________________________<o:p></o:p></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt">Patricia Grob, Ph.D.<br>
Research Specialist<br>
Nogales Lab<br>
HHMI at UC Berkeley<br>
Molecular & Cell Biology Department / QB3,<br>
Stanley Hall #742<br>
University of California, Berkeley<br>
Berkeley, CA 94720-3220<br>
Phone: (510)666-3335<span class="apple-tab-span"> </span> Fax: (510)666-3336<br>
E.M. suite B307: (510)666-3339<br>
URL: <a href="https://urldefense.com/v3/__https://eur02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__http*3A*2F*2Fcryoem.berkeley.edu__*3B!!Mih3wA!C7DcZr7wzSTHKwpZuUfXknBo_VK608jMkHM0LJNxPRO0sFyRNtyFeiQhOVGG_ZYiKbC1QD2tjWmplJUuHY_C*24&data=05*7C01*7Ckehf*40plen.ku.dk*7C3e8258815e63412779b708dafefce2ab*7Ca3927f91cda14696af898c9f1ceffa91*7C0*7C0*7C638102659352599083*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=ykvEO3nH0hTzRXJRWb8XEtrY2J4Gwcz0vujgTova75k*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!BLmW-AaYfjHJAxYdqeeuqnwB76eMzTJqZrF4cCF41tY6QgiP6rnysvBnYJlxF_86kn6fGUKYpLIqZkf0qg$">
cryoem.berkeley.edu</a><br>
<a href="mailto:pgrob@berkeley.edu">Email: pgrob@berkeley.edu</a><br>
<br>
<o:p></o:p></p>
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<p class="MsoNormal"><br>
<br>
<o:p></o:p></p>
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<p class="MsoNormal">On Jan 24, 2023, at 2:43 PM, Christopher Winkler <<a href="mailto:microwink@gmail.com">microwink@gmail.com</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Hey Ed, <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">We went through a similar cost benefit analysis of plasma cleaners after our Solarus RF board failed. We went with one of the more basic plasma cleaners partly due to budget, partly due to the simplicity of operation of the more basic units,
and partly due to the simplicity of design of the basic units. As you point out, the cost of a "simple" cleaner is 1/4 to 1/3 of the Solarus II. The Solarus was pretty easy to operate and the ability to save recipes is nice, but the more basic plasma cleaners
are even simpler--you basically toggle a switch to start or stop the cleaner. There is no ability to save a recipe on our cleaner but we only had a few saved recipes on our Solarus that were routinely used. It's not hard to adjust time and power on the basic
units. Most importantly for me, the more basic cleaners forego the TMP for a basic dry scroll pump so there are no diaphragm pumps to rebuild or turbo oil bearings to swap, and there's no painful teardown of the system to access the vacuum systems. The poorer
vacuum is fully sufficient to generate a good quality plasma and I've not really seen much of a difference in cleaning performance--the new "simple" model does a good job of cleaning and removing hydrocarbon contamination (we don't use it for glow discharging
or treating grid surfaces, so I can't comment on that). <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">A downside of the basic cleaner is that it only uses one process gas at a time, so you cannot switch between argon and oxygen mixtures or hydrogen like the Solarus. This is not a problem for our application but it's something to consider. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Happy to discuss specifics off of the listserv,<o:p></o:p></p>
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<p class="MsoNormal">Chris<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Tue, Jan 24, 2023 at 5:17 PM Egelman, Edward H (ehe2n) <<a href="mailto:egelman@virginia.edu">egelman@virginia.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">We have a Gatan Solarus that is failing, and are thinking about replacements. The newer Solarus II is from 5-10 times more expensive than the simplest plasma cleaners out there.
Does anyone have any comparisons to justify this very high price tag?<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Thanks,<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Ed<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt"> </span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt"> </span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">Edward Egelman</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">Harrison Distinguished Professor,</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">Department of Biochemistry and Molecular Genetics</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">Professor of Molecular Physiology and Biological Physics</span><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">University of Virginia</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">phone: 434-924-8210</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><span style="font-size:10.5pt">fax: 434-924-5069</span><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><a href="mailto:egelman@virginia.edu" target="_blank"><span style="font-size:10.5pt">egelman@virginia.edu</span></a><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><a href="https://urldefense.com/v3/__https://eur02.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fegelmanlab.org*2F__*3B!!Mih3wA!E1FbZMjPylp2E_W95tkmjXBa_SrdglqlKHumjIYttQDrp5j2mFygiYldlXjLxNN29KbT4EBRw_E2__08r2FUsiA*24&data=05*7C01*7Ckehf*40plen.ku.dk*7C3e8258815e63412779b708dafefce2ab*7Ca3927f91cda14696af898c9f1ceffa91*7C0*7C0*7C638102659352599083*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=MkpQtTMHyTBqrF*2F4NnZuGtB6iOtz6G30fonet6hSDQc*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!BLmW-AaYfjHJAxYdqeeuqnwB76eMzTJqZrF4cCF41tY6QgiP6rnysvBnYJlxF_86kn6fGUKYpLLZDDpgcQ$" target="_blank"><span style="font-size:10.5pt">https://egelmanlab.org/</span></a><o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"> <o:p></o:p></p>
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<p class="MsoNormal">_______________________________________________<br>
3dem mailing list<br>
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<a href="https://urldefense.com/v3/__https://eur02.safelinks.protection.outlook.com/?url=https*3A*2F*2Fmail.ncmir.ucsd.edu*2Fmailman*2Flistinfo*2F3dem&data=05*7C01*7Ckehf*40plen.ku.dk*7C3e8258815e63412779b708dafefce2ab*7Ca3927f91cda14696af898c9f1ceffa91*7C0*7C0*7C638102659352599083*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=qY3VsbWvGUqc8jLjhqQFQckhFRQchTk1GjnG0M6rKSE*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!BLmW-AaYfjHJAxYdqeeuqnwB76eMzTJqZrF4cCF41tY6QgiP6rnysvBnYJlxF_86kn6fGUKYpLLkpBaGbQ$" target="_blank">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><o:p></o:p></p>
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<p class="MsoNormal">_______________________________________________<br>
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<a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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