<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
</head>
<body>
<div class="moz-cite-prefix">Hi,</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">maybe a very simple solution: Place a
pleated filter paper next to the grids, add 50 µl Amylamine and
pump and glow-discharge as usual ... you should notice a change in
the color of the plasma. <br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Cheers,</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">Thorsten</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix"><br>
</div>
<div class="moz-cite-prefix">On 12.01.2023 12:04, Farzad wrote:<br>
</div>
<blockquote type="cite"
cite="mid:CAM-VoOm-kXJ8nvoONv4v6VX+XcWpkLdA_dnOsAdYPJwnVLht2A@mail.gmail.com">
<meta http-equiv="content-type" content="text/html; charset=UTF-8">
<div dir="ltr">Dear David,
<div><br>
</div>
<div>Let me share my experience with semiconductor gas systems.</div>
<div><br>
</div>
<div>For supplying the gas from a liquid source like amylamine
in a vacuum system like Pelco easiGlow, you need a "precursor
gas generator". Despite its fancy name, it can be a very
simple setup. In fact, this setup is very common in various
systems like Chemical Vapor Deposition (CVD) and maybe you
have already seen it in the environmental scanning electron
microscopes (for water vapor supply or some liquid precursors
for EBID, etc). In this set up you keep the liquid in a closed
vessel like a glass balloon, and you keep the temperature
around it fixed. The temperature determines the partial
pressure of the gas which is necessary for the repeatability
of the process. Normally, people put the balloon in a
laboratory balloon heater or an ice bath, depending on the
boiling point of the precursor and the desired partial
pressure. The selection of the temperature is up to you. If
your lab environment is stable enough and your gas consumption
is also low enough, you can do it at room temperature without
any additional device. In this case, you just need to have a
big enough balloon and leave it to equilibrate. </div>
<div><br>
</div>
<div>Also, for more repeatability, you are advised to keep the
pressure over the liquid vessel (the balloon) at a constant
level. The reason is that for non-ideal gasses the partial
pressure is slightly dependent on the system's pressure. For
achieving that level of stability, you can set up a carrier
gas inlet to the balloon like a bobbler. The carrier gas can
be nitrogen, argon, or even air (just leave an open inlet to
the bobbler. You can also keep balloons under a vacuum
condition which is also another strategy for keeping the
pressure constant! In this case, the setup is similar except
the carrier gas pipe is not submerged in the liquid and it is
connected to a vacuum pump.<br>
</div>
<div><br>
</div>
<div><b>Caution 1: There is a safety consideration for heating
the vessel to near boiling point or working at high carrier
gas pressure. If you want to have high partial pressure and
hence you decide to heat the precursor to near its boiling
temperature you need to consider a safety release valve and
a reliable suction hood OR a higher pressure chamber. It is
worthy of note that since evaporation needs time and energy,
heating is always preferred for a more uniform gas
composition. Glass balloons are not made to maintain very
high pressures.</b><br>
</div>
<div><b><br>
</b></div>
<div><b>Caution 2: Should you need to inject multiple gasses you
need to install mass flow controllers at the inlets after
the bobblers. This guarantees a constant gas composition.</b></div>
<div><b><br>
</b></div>
<div>Hope these details help you make your bobbler (precursor
gas generator).</div>
<div><br>
</div>
<div>Cheers,</div>
<div><br>
</div>
<div>Ferrie</div>
<div><b><br>
</b></div>
<div>
<div>
<div dir="ltr" class="gmail_signature"
data-smartmail="gmail_signature">
<div dir="ltr">
<div style="color:rgb(0,0,0)">Farzad Hamdi, Dr.-Ing</div>
<div style="color:rgb(0,0,0)">Scientist,</div>
<div style="color:rgb(0,0,0)">Kastritis Laboratory for
Biomolecular Research</div>
<div style="color:rgb(0,0,0)"><span
style="font-family:Helvetica,Arial,sans-serif;font-size:13px">Interdisciplinary
research center HALOmem, </span></div>
<div style="color:rgb(0,0,0)"><span
style="font-family:Helvetica,Arial,sans-serif;font-size:13px">Institute
of Biochemistry and Biotechnology, </span><span
style="font-family:Helvetica,Arial,sans-serif;font-size:13px">Biocenter, </span></div>
<div style="color:rgb(0,0,0)"><span
style="font-family:Helvetica,Arial,sans-serif;font-size:13px">Martin
Luther University Halle-Wittenberg, Halle (Saale),
Germany</span></div>
<div style="color:rgb(0,0,0)">----------------------------------------------------------------------------------------</div>
<div style="color:rgb(0,0,0)">Martin-Luther-Universität
Halle-Wittenberg</div>
<div style="color:rgb(0,0,0)">Biozentrum, Room A.2.14
& 15</div>
<div style="color:rgb(0,0,0)">IWE ZIK HALOmem NWG III</div>
<div style="color:rgb(0,0,0)">"Kryo-Elektronenmikroskopie
an Membranproteinkomplexen"</div>
<div style="color:rgb(0,0,0)">Weinbergweg 22, 06120
Halle</div>
<div style="color:rgb(0,0,0)">Telefone:</div>
<div style="color:rgb(0,0,0)">Office: +49 345 5524984</div>
<div style="color:rgb(0,0,0)">Glacios Room: +49 345
5524883</div>
<div style="color:rgb(0,0,0)">JEM-3200FSC Room: +49 345
5524868 </div>
<div style="color:rgb(0,0,0)">Cellphone:
+49-1525-6841873</div>
<div style="color:rgb(0,0,0)">Web (Lab): <a
href="https://urldefense.com/v3/__https://blogs.urz.uni-halle.de/kastritislab/__;!!Mih3wA!CrYJf8iR8lzAKlzlLYMatgfq2Q6KT3HyN_t3kxVMZXiANDgr0LHslgwzxftwcRObZzYwQkdQ4r5KjjatZw$"
target="_blank" moz-do-not-send="true">https://blogs.urz.uni-halle.de/kastritislab/</a></div>
<div style="color:rgb(0,0,0)">Web (HALOmem): <a
href="https://urldefense.com/v3/__https://www.halomem.de/en/__;!!Mih3wA!CrYJf8iR8lzAKlzlLYMatgfq2Q6KT3HyN_t3kxVMZXiANDgr0LHslgwzxftwcRObZzYwQkdQ4r6fJjYGWw$"
target="_blank" moz-do-not-send="true">https://www.halomem.de/en/</a></div>
<div style="color:rgb(0,0,0)"><br>
</div>
<div style="color:rgb(0,0,0)"><span
style="color:rgb(34,34,34)">Achtung: Falls Sie diese
E-Mail auf Deutsch erhalten, bedenken Sie bitte,
dass sie mit dem Deepl AI Übersetzer übersetzt
werden könnte.</span><br>
</div>
<div><br>
</div>
</div>
</div>
</div>
<br>
</div>
</div>
<br>
<div class="gmail_quote">
<div dir="ltr" class="gmail_attr">On Wed, Jan 11, 2023 at 10:43
PM Morgan, David Gene <<a
href="mailto:dagmorga@indiana.edu" moz-do-not-send="true"
class="moz-txt-link-freetext">dagmorga@indiana.edu</a>>
wrote:<br>
</div>
<blockquote class="gmail_quote" style="margin:0px 0px 0px
0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
<div class="msg-6160933283769279806">
<div dir="ltr">
<div><span
style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)">Hi,</span></div>
<div><span
style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)"><br>
</span></div>
<div><span
style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)">Has
anyone modified a Pelco glow discharge device so that
one can introduce something like amylamine into the
chamber during the glow discharge? I can think of
several ways that this could be done, but if someone
already has a working model, I would love to chat with
them.</span></div>
<div><span
style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)"><br>
</span></div>
<div><span
style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0);background-color:rgb(255,255,255)">Thanks
in advance.<br>
</span></div>
<div>
<div
style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"><br>
</div>
<div id="m_-6160933283769279806Signature">
<div>
<div name="divtagdefaultwrapper">
<div name="divtagdefaultwrapper">
-- </div>
<div name="divtagdefaultwrapper">
politics is more difficult than physics.</div>
<div name="divtagdefaultwrapper">
A.
Einstein</div>
<div name="divtagdefaultwrapper">
<br>
<div name="divtagdefaultwrapper">
David Gene Morgan<br>
Electron Microscopy Center<br>
047E Simon Hall<br>
IU Bloomington<br>
812 856 1457 (office)<br>
812 856 3221 (3200)<br>
<a
href="https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!E-uOhSgxgNYw4mUI1_qwM92G9bUzQP-dODh_oYlLut6LdZSIomx5L21fr9mJVoO_NlCT0-fIASon8IjMfkYK0cs$"
target="_blank" moz-do-not-send="true">http://iubemcenter.indiana.edu</a></div>
</div>
</div>
</div>
</div>
</div>
</div>
_______________________________________________<br>
3dem mailing list<br>
<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank"
moz-do-not-send="true" class="moz-txt-link-freetext">3dem@ncmir.ucsd.edu</a><br>
<a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem"
rel="noreferrer" target="_blank" moz-do-not-send="true"
class="moz-txt-link-freetext">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br>
</div>
</blockquote>
</div>
<br>
<fieldset class="moz-mime-attachment-header"></fieldset>
<pre class="moz-quote-pre" wrap="">_______________________________________________
3dem mailing list
<a class="moz-txt-link-abbreviated" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>
<a class="moz-txt-link-freetext" href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a>
</pre>
</blockquote>
<p><br>
</p>
<pre class="moz-signature" cols="72">--
*******************************************
Dr. Thorsten Mielke
- Head Microscopy & Cryo Electron Microscopy Service Group -
Max Planck Institute for Molecular Genetics
Ihnestr. 63-73
D-14195 Berlin
Tel.: +49-(0)30 8413-1644
Fax: +49-(0)30 8413-1385
E-mail: <a class="moz-txt-link-abbreviated" href="mailto:mielke@molgen.mpg.de">mielke@molgen.mpg.de</a></pre>
</body>
</html>