<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto">Hi David and all the colleagues who replied off-line,<div><br></div><div>Thank you all for sharing your experience!</div><div><br></div><div>The feedbacks I have received so far suggest ultrathin carbon coated grids do not lead to excessive drift/motion unless the grid is tilted for data acquisition. Interestingly, our microscope did not seem to be the cause of the excessive drift/motion because no similar drift/motion was observed with uncoated grids. Tests on thick carbon regions of coated grids did not show similar drift/motion either. We have ordered new ultrathin carbon coated grids. I will post updates once we use the new grids.</div><div><br></div><div>Feedbacks are still welcome and will be appreciated.</div><div><br></div><div>Best,</div><div>Guobin</div><div><br></div><div><br><br><div dir="ltr">Sent from my iPhone, please excuse typos</div><div dir="ltr"><br><blockquote type="cite">On Nov 22, 2022, at 1:33 PM, David Michael Belnap <David.Belnap@utah.edu> wrote:<br><br></blockquote></div><blockquote type="cite"><div dir="ltr">
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<p class="MsoNormal"><span style="font-family:"Georgia",serif">Guobin,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Georgia",serif">I have used 671-200-Cu grids from Ted Pella several times without problems. I don’t recall ever having drift problems. I was imaging virus or virus-like particles on these grids. 671-200-Cu grids
are Quantifoil R2/1 grids coated with 2 nm C.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-family:"Georgia",serif">David<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.5pt;font-family:"Georgia",serif;color:black">=======================================<br>
David M. Belnap<br>
Electron Microscopy Core Laboratory<br>
Department of Biochemistry<br>
School of Biological Sciences<br>
2501 SMBB<br>
University of Utah<br>
Salt Lake City, Utah 84112 USA<br>
Phone 801.585.1242<br>
FAX 801.587.3077<br>
</span><span style="font-family:"Georgia",serif;color:black"><a href="mailto:David.Belnap@utah.edu" target="_blank" title="mailto:David.Belnap@utah.edu"><span style="font-size:11.5pt;color:#0563C1">David.Belnap@utah.edu</span></a></span><span style="font-size:11.5pt;font-family:"Georgia",serif;color:black"><br>
=======================================</span><span style="color:black"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Guobin Hu <gb_hu@yahoo.com><br>
<b>Date: </b>Friday, November 18, 2022 at 8:22 PM<br>
<b>To: </b>3DEM Mailing List <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[3dem] Excessive drift/motion on 2nm ultrathin carbon coated grids<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Helvetica Neue"">I am collecting data with 2nm ultrathin carbon coated Quantifoil holy grids and observing excessive/abnormal drift/motion in images which I did not see with regular Quantifoil
grids or other type of grids. There is no indication of the microscope as the cause of drift/motion. I am curious if anyone else has also observed the similar motion/drift with 2nm ultrathin carbon coated grids. FFTs w/ and w/o drift/motion are attached as
references. Any feedback insight will be appreciated.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Helvetica Neue"">Thanks,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Helvetica Neue"">Guobin<o:p></o:p></span></p>
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