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--></style></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal>Hi Tim,<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I agree 100% with Dennis.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Basically, baking any Gatan cryo holder, e.g., 626, 636, Elsa 698 above 100°C, i.e., 150°C, could mean expensive repairs for the holder. There are components soldered to the internal thermal conductor that are likely to be damaged if the holder is baked at elevated temperatures(above 100°C). <o:p></o:p></p><p class=MsoNormal>I would stick with the 100°C bake out temperature, unless of course the Elsa’s user’s manual specifies otherwise.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>I hope this helps.<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal style='text-autospace:none'>Best,<o:p></o:p></p><p class=MsoNormal style='text-autospace:none'>Tom<o:p></o:p></p><p class=MsoNormal style='text-autospace:none'><o:p> </o:p></p><p class=MsoNormal style='margin-left:48.0pt;text-indent:-48.0pt;text-autospace:none'>Tom Schmelzer<o:p></o:p></p><p class=MsoNormal><b><span style='color:#C00000'>TGS Technologies LLC<o:p></o:p></span></b></p><p class=MsoNormal><b><span style='color:#C00000'>USA<o:p></o:p></span></b></p><p class=MsoNormal>724-453-3865<o:p></o:p></p><p class=MsoNormal>email: <a href="mailto:tom@tgstechnologies.net"><span style='color:#0563C1'>tom@tgstechnologies.net</span></a><o:p></o:p></p></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><o:p> </o:p></p><div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b><span style='font-size:12.0pt;color:black'>From: </span></b><span style='font-size:12.0pt;color:black'>3dem-bounces <3dem-bounces@ncmir.ucsd.edu> on behalf of "dennis.winkler@nih.gov" <dennis.winkler@nih.gov><br><b>Date: </b>Tuesday, August 30, 2022 at 11:35 AM<br><b>To: </b>Tim Gruene <tim.gruene@univie.ac.at><br><b>Cc: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br><b>Subject: </b>Re: [3dem] [EXTERNAL] Re: Elsa 698 : best-practice bake-out procedure<o:p></o:p></span></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><p class=MsoNormal>Hi Tim,<o:p></o:p></p><p class=MsoNormal> <o:p></o:p></p><p class=MsoNormal>For what it is worth, I seem to recall being told many years ago that over time the construction of cryoholders changed (perhaps the type of neck epoxy used?) and the recommended bake temperature changed with it.<o:p></o:p></p><p class=MsoNormal> <o:p></o:p></p><p class=MsoNormal>Of course there are always the “more is better” folks, but I think it best to stick to the temperature recommendation and we use 100C.<o:p></o:p></p><p class=MsoNormal> <o:p></o:p></p><p class=MsoNormal>Best,<o:p></o:p></p><div><p class=MsoNormal>- Dennis<o:p></o:p></p><p class=MsoNormal>---------------------------------<o:p></o:p></p><p class=MsoNormal>Dennis C. Winkler, PhD<o:p></o:p></p><p class=MsoNormal>National Institutes of Health<o:p></o:p></p><p class=MsoNormal>NIDCD<o:p></o:p></p><p class=MsoNormal>35A Convent Drive<o:p></o:p></p><p class=MsoNormal>Bldg. 35A, Room 1F-127, MSC-3745<o:p></o:p></p><p class=MsoNormal>Bethesda, MD 20892-3745, USA<o:p></o:p></p><p class=MsoNormal>Phone: (301) 451-7220<o:p></o:p></p><p class=MsoNormal>Email: Dennis.Winkler@nih.gov<o:p></o:p></p></div><p class=MsoNormal> <o:p></o:p></p><div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal style='margin-left:.5in'><b><span style='font-size:12.0pt;color:black'>From: </span></b><span style='font-size:12.0pt;color:black'>3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Tim Gruene <tim.gruene@univie.ac.at><br><b>Organization: </b>University of Vienna<br><b>Date: </b>Tuesday, August 30, 2022 at 5:34 AM<br><b>To: </b>Andrei Moiseenko <postmoiseenko@gmail.com><br><b>Cc: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br><b>Subject: </b>[EXTERNAL] Re: [3dem] Elsa 698 : best-practice bake-out procedure</span><o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>Hi Andrei,<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>thanks a lot for sharing these details!<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>I was mostly curious about frequencies of the various steps (just<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>pumping / pumping and baking), and the temperature. My former PhD<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>student, Julian Wennmacher, would bake out zeolites at much higher<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>temperatures, and I would have guessed for 150C. So it is good to know<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>100C is recommended.<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>Best,<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>Tim<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>On Mon, 29 Aug 2022 22:48:21 +0300 Andrei Moiseenko<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'><<a href="mailto:postmoiseenko@gmail.com">postmoiseenko@gmail.com</a>> wrote:<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'> <o:p></o:p></p></div><blockquote style='border:none;border-left:solid #B5C4DF 4.5pt;padding:0in 0in 0in 4.0pt;margin-left:3.75pt;margin-top:5.0pt;margin-right:0in;margin-bottom:5.0pt' id="MAC_OUTLOOK_ATTRIBUTION_BLOCKQUOTE"><div><p class=MsoNormal style='margin-left:.5in'>Hi Tim, we use Elsa 698 with the Gatan 1905 controller and 655 pumping<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>station. The bakeout temperature is kept at 100C, never exceeding it<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>for more than a few degrees. We usually perform overnight bakeout<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>once a week, but we pump out the holder dewar after each cryo-day. I<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>think that it is ok for the holder to perform bakeout more often in<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>case of extensive sample exchange. You definitely need to do the<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>holder bakeout if: 1) The holder fails to reach the -170 C<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>temperature within reasonable time. In our case ~45-50 min inside the<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>pumping station, but may be different for your holder.<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>2) The holder cannot keep the cold temperature during operation<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>3) The frost or condensate forms on the holder dewar<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>4) The holder was not used for a couple of weeks.<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>In case the holder was not used for a longer time or in case the<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>overnight bakeout does not solve the temperature problem, the longer<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>bakeout times should be used, like 48h or 72h.<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>пн, 29 авг. 2022 г. в 15:52, Tim Gruene <<a href="mailto:tim.gruene@univie.ac.at">tim.gruene@univie.ac.at</a>>:<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> Dear all,<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> we recently received our Gatan Elsa 698 holder. I would like to<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> gather some recommendations for daily use and bake-out procedure.<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> - what temperature do you use for bake-out?<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> - how long do you bake-out for?<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> - are there special maintenance procedures?<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> - what would be signs of deterioriation, abd after what period would<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> they occur?<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> We use the holder both for room-temperature and for<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> cryo-measurements.<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> The manual refers to the Gatan pumping station, but ours was<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> delivered with a Pfeiffer pumping station ;-)<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> Thanks a lot for your time!<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> Best,<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> Tim<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> <o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> --<o:p></o:p></p></div><div><p class=MsoNormal style='margin-left:.5in'>> --<o:p></o:p></p></div><div><p class=MsoNormal 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