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Hi all,</div>
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We are trying to image tagged VLPs in cells using Leica cryo CLEM. Our sample looks good under the regular confocal microscope with bright particles. However, the cell fluorescence background is relatively high <span style="background-color:rgb(255, 255, 255);display:inline !important">u</span><span style="margin:0px;background-color:rgb(255, 255, 255);display:inline !important">nder
the Leica cryo CLEM microscopy.</span></div>
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We have tried using FluoroBrite DMEM media or different cell lines but the problem is still there. I was wondering if anyone had a similar problem when imaging cell samples using cryo CLEM? Thank you.</div>
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Best wishes,</div>
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Juan</div>
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<p style="font-size:11pt; font-family:Calibri,sans-serif; margin:0px"><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif">-----------------------------------------------</span></p>
<p style="font-size:11pt; font-family:Calibri,sans-serif; margin:0px"><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif">Juan Shen</span></p>
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<p style="font-size:11pt; font-family:Calibri,sans-serif; margin:0px"><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif"> </span></p>
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<p style="font-size:11pt; font-family:Calibri,sans-serif; margin:0px"><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif">Lab manager of Prof Peijun Zhang's group</span></p>
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<p style="font-size:11pt; font-family:Calibri,sans-serif; margin:0px"><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif">Wellcome Centre for Human Genetics</span><span style="margin:0px; font-size:10pt"><br>
</span><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif">University of Oxford</span><span style="margin:0px; font-size:10pt"><br>
</span><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif">Old Road Campus</span><span style="margin:0px; font-size:10pt"><br>
</span><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif">Roosevelt Drive, Oxford OX3 7BN</span></p>
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<p style="font-size:11pt; font-family:Calibri,sans-serif; margin:0px"><span style="margin:0px; font-size:10pt; font-family:Arial,Helvetica,sans-serif">Tel: 01865 287723</span></p>
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