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<p class="MsoNormal"><span style="font-size:12.0pt">Hello all,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">I am observing bizarre artefacts—vertical streaks and arrays of spots (attached)—in Fourier transforms calculated from EPU’s averaged .mrc files during CTF estimation with Gctf v1.06 (via RELION-3.1.0). With
Ctffind or after conversion to and from EMAN2’s .hdf format, all seems normal.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Is there a known incompatibility between EPU’s .mrc format and Gctf, or could these artefacts be caused by something else? Are there any alternative solutions to converting to and from .hdf?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt">Thank you, <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal" style="line-height:120%;text-autospace:none"><b><span style="font-size:12.0pt;line-height:120%;color:#1E6B52">James L. Kizziah, PhD | Cryo-EM Facility Manager<o:p></o:p></span></b></p>
<p class="MsoNormal" style="line-height:120%;text-autospace:none"><span style="font-size:12.0pt;line-height:120%">Department of Microbiology<o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:120%;text-autospace:none"><span style="font-size:12.0pt;line-height:120%">UAB | The University of Alabama at Birmingham<o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:120%;text-autospace:none"><span style="font-size:12.0pt;line-height:120%">SHEL B40 | 1825 University Blvd | Birmingham, AL 35233<o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:120%;text-autospace:none"><b><span style="font-size:12.0pt;line-height:120%;color:#1E6B52"><a href="mailto:kizziah4@uab.edu"><span style="color:#1E6B52;text-decoration:none">kizziah4@uab.edu</span></a>
</span></b><span style="font-size:12.0pt;line-height:120%">| Phone: (205) 975-3376 |
</span><span style="font-size:12.0pt;line-height:120%">Pronouns: he/him/his</span><span style="font-size:12.0pt;line-height:120%"><o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:120%;text-autospace:none"><b><span style="font-size:12.0pt;line-height:120%;color:#1E6B52"><a href="https://urldefense.com/v3/__https://www.linkedin.com/in/jameskizziah/__;!!Mih3wA!EgD2Pem7dzaCg6x0l8DebdfyW7Idf0r407R_LIaZGpAsYnCqHBcDGEAgmwLfwHT1nvu7tBrGQ116PjIlXzLR$"><span style="color:#1E6B52;text-decoration:none">LinkedIn</span></a></span></b><span style="font-size:12.0pt;line-height:120%">
|<span style="color:#1E6B52"> </span></span><b><span style="font-size:12.0pt;line-height:120%;color:#1E6B52"><a href="https://urldefense.com/v3/__https://www.name-coach.com/james-kizziah__;!!Mih3wA!EgD2Pem7dzaCg6x0l8DebdfyW7Idf0r407R_LIaZGpAsYnCqHBcDGEAgmwLfwHT1nvu7tBrGQ116PjWY5qAx$"><span style="color:#1E6B52;text-decoration:none">Name Pronunciation</span></a></span></b><span style="font-size:12.0pt;line-height:120%"><o:p></o:p></span></p>
<p class="MsoNormal" style="line-height:120%;text-autospace:none"><span style="font-size:12.0pt;line-height:120%"><o:p> </o:p></span></p>
<p class="MsoNormal" style="line-height:120%;text-autospace:none"><b><span style="font-size:12.0pt;line-height:120%;color:#1E6B52"><a href="https://urldefense.com/v3/__https://www.uab.edu/medicine/home__;!!Mih3wA!EgD2Pem7dzaCg6x0l8DebdfyW7Idf0r407R_LIaZGpAsYnCqHBcDGEAgmwLfwHT1nvu7tBrGQ116PgecOyKH$" target="_blank"><span style="color:#1E6B52;text-decoration:none">uab.edu/medicine</span></a></span></b><b><span style="font-size:12.0pt;line-height:120%;color:#1E6B52"><o:p></o:p></span></b></p>
<p class="MsoNormal"><o:p> </o:p></p>
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