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Hi David,
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<div class="">Maybe goCTF is the program you were thinking of?</div>
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<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__doi.org_10.1016_j.jsb.2018.11.012&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=slGb8qvejuUkwBNM1mgPL-0gCnPHmJJ2Zj3q5MonWalh7vus3gD1UU2XGd56_BuN&s=2UytSWYXa0FiSTVdn_nrITkww2QIVzXprwR20jBFk-w&e=" class="">https://doi.org/10.1016/j.jsb.2018.11.012</a></div>
<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.lsi.umich.edu_science_centers-2Dtechnologies_cryo-2Delectron-2Dmicroscopy_research_goctf&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=slGb8qvejuUkwBNM1mgPL-0gCnPHmJJ2Zj3q5MonWalh7vus3gD1UU2XGd56_BuN&s=fvtxnCWeWPMrMi33oRmsfyPbPzYd3x3KuClHo6_hPbI&e=" class="">https://www.lsi.umich.edu/science/centers-technologies/cryo-electron-microscopy/research/goctf</a></div>
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<div class="">Cheers,</div>
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Guillaume</div>
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<div class="">On 28 Feb 2022, at 23:50, Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" class="">dagmorga@indiana.edu</a>> wrote:</div>
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Daniel,</div>
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The problem is that the tilt causes the higher resolution Thon rings to become out-of-phase, and so the standard programs can't give a decent estimate of the goodness of-fit. I vaguely remember that someone had a program that took the tilt into account a number
of years ago, but a quick hunt for it didn't turn up anything.<br class="">
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-- </div>
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politics is more difficult than physics.</div>
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David Gene Morgan<br class="">
Electron Microscopy Center<br class="">
047E Simon Hall<br class="">
IU Bloomington<br class="">
812 856 1457 (office)<br class="">
812 856 3221 (3200)<br class="">
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<font face="Calibri, sans-serif" style="font-size: 11pt;" class=""><b class="">From:</b><span class="Apple-converted-space"> </span>Daniel Asarnow <<a href="mailto:asarnow@msg.ucsf.edu" class="">asarnow@msg.ucsf.edu</a>><br class="">
<b class="">Sent:</b><span class="Apple-converted-space"> </span>Monday, February 28, 2022 5:45 PM<br class="">
<b class="">To:</b><span class="Apple-converted-space"> </span>Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" class="">dagmorga@indiana.edu</a>><br class="">
<b class="">Cc:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><span class="Apple-converted-space"> </span><<a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a>><br class="">
<b class="">Subject:</b><span class="Apple-converted-space"> </span>[External] Re: [3dem] tilted transfer functions?</font>
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<div class="">I think any patch-based CTF program, like the one in cryosparc or gCTF's local mode (with evenly spaced coordinates instead of real particle locations) would work well. With cryoSPARC there's an easy method to plot the tilt axis; you can also
choose a specific number X and Y divisions for the patches.</div>
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<div class="">Best,</div>
<div class="">-da<br class="">
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<div dir="ltr" class="x_gmail_attr">On Mon, Feb 28, 2022 at 2:41 PM Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" class="">dagmorga@indiana.edu</a>> wrote:<br class="">
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Hi,</div>
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What's the best way to evaluate the CTF of an image that has a significant amount of tilt?<br class="">
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<div name="x_divtagdefaultwrapper" class="">-- </div>
<div name="x_divtagdefaultwrapper" class=""> politics is more difficult than physics.</div>
<div name="x_divtagdefaultwrapper" class=""> A. Einstein</div>
<div name="x_divtagdefaultwrapper" class=""><br class="">
<div name="x_divtagdefaultwrapper" class=""> David Gene Morgan<br class="">
Electron Microscopy Center<br class="">
047E Simon Hall<br class="">
IU Bloomington<br class="">
812 856 1457 (office)<br class="">
812 856 3221 (3200)<br class="">
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