<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=us-ascii">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
Its been called to my attention that the https link for <a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__eman2.org&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=BcJ0NnTcdUXjBRj34OKRldagCVvbHeJ0FH60r3AX5oo&e=" class="">
eman2.org</a> seems to be broken at the moment. Both addresses should be fine with http: or here are the direct links:
<div class=""><br class="">
</div>
<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__blake.bcm.edu_emanwiki_EMAN2_&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=AteG0tSoom8YKukj6_V4itZUBQRNG9OSLMSO8UFpjFI&e=" class="">https://blake.bcm.edu/emanwiki/EMAN2/</a></div>
<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__blake.bcm.edu_emanwiki_EMAN2_Programs_e2filtertool&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=8yWzZ9LrhktjQFihHs8zFS7VXUi2kfmn3OI2uxc_zN4&e=" class="">https://blake.bcm.edu/emanwiki/EMAN2/Programs/e2filtertool</a></div>
<div class=""><br class="">
<div class="">
<div dir="auto" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="text-align: start; text-indent: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div style="text-align: start; text-indent: 0px;">
<div>
<div style="font-family: monospace; font-size: 14px;" class="">
<div class="" style="font-family: Helvetica; font-size: 18px;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div class="" style="font-family: monospace; font-size: 14px;">--------------------------------------------------------------------------------------<br class="">
Steven Ludtke, Ph.D. <<a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a>>                      Baylor College of Medicine </div>
<div class="" style="font-family: monospace; font-size: 14px;">Charles C. Bell Jr., Professor of Structural Biology<br class="">
Dept. of Biochemistry and Molecular Biology                      (<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bcm.edu_biochem&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=O2SdoE4seda4rmK0RJaAt8KMEv8wseBCc_T7A8I1U8A&e=" class="">www.bcm.edu/biochem</a>)</div>
<div class="" style="font-family: monospace; font-size: 14px;">Academic Director, CryoEM Core                                        (<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__cryoem.bcm.edu&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=IKY_EKlR_NN5HQRRnjiXRFSQeXpDdeGhjli5uboGYnU&e=" class="">cryoem.bcm.edu</a>)<br class="">
Co-Director CIBR Center                                    (<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bcm.edu_research_cibr&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=lMOb5nHmEQpAJGOtaTfL_r3ffQ2yd1W9KSQz46o4XIQ&e=" class="">www.bcm.edu/research/cibr</a>)<br class="">
</div>
<div class="" style="font-family: monospace; font-size: 14px;"><br class="">
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
<br class="Apple-interchange-newline">
<br class="Apple-interchange-newline">
</div>
<div><br class="">
<blockquote type="cite" class="">
<div class="">On Jan 31, 2022, at 12:21 PM, Ludtke, Steven J. <<a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
Hi Wu,
<div class="">EMAN2 (<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__eman2.org_&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=0oseEn20qxbGoLAgZ_1HMKs5ozBkV5ffHUzBtxs0W0g&e=" class="">https://eman2.org</a>) includes options for installing on Windows machines, though we prefer the WSL2 approach, there are native binaries as well, which can be used for standard EM image processing
 operations. See also</div>
<div class=""><br class="">
</div>
<div class=""><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__eman2.org_Programs_e2filtertool&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=Obb8O679SewxYF_pP1dURUd2oZmj8SG4s_Cz_TVB0B0&e=" class="">https://eman2.org/Programs/e2filtertool</a></div>
<div class=""><br class="">
</div>
<div class=""><br class="">
<div class="">
<div dir="auto" style="caret-color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="text-align: start; text-indent: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div dir="auto" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">
<div style="text-align: start; text-indent: 0px;" class="">
<div class="">
<div style="font-family: monospace; font-size: 14px;" class="">
<div class="" style="font-family: Helvetica; font-size: 18px;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
<div class="" style="font-family: monospace; font-size: 14px;">--------------------------------------------------------------------------------------<br class="">
Steven Ludtke, Ph.D. <<a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a>>                      Baylor College of Medicine </div>
<div class="" style="font-family: monospace; font-size: 14px;">Charles C. Bell Jr., Professor of Structural Biology<br class="">
Dept. of Biochemistry and Molecular Biology                      (<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bcm.edu_biochem&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=O2SdoE4seda4rmK0RJaAt8KMEv8wseBCc_T7A8I1U8A&e=" class="">www.bcm.edu/biochem</a>)</div>
<div class="" style="font-family: monospace; font-size: 14px;">Academic Director, CryoEM Core                                        (<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__cryoem.bcm.edu_&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=FkGsyZkREc_y5SErg2NaMVsmAwf9aCcZAS75A5PqcNo&e=" class="">cryoem.bcm.edu</a>)<br class="">
Co-Director CIBR Center                                    (<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.bcm.edu_research_cibr&d=DwMFAg&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=QgOGzDsX582VVaKhhEb5qN1SpApuP-lZTRM8ZLYMoRPLlKxqC-sWIj7JUoAmY5sR&s=lMOb5nHmEQpAJGOtaTfL_r3ffQ2yd1W9KSQz46o4XIQ&e=" class="">www.bcm.edu/research/cibr</a>)<br class="">
</div>
<div class="" style="font-family: monospace; font-size: 14px;"><br class="">
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
</div>
<br class="Apple-interchange-newline">
<br class="Apple-interchange-newline">
</div>
<div class=""><br class="">
<blockquote type="cite" class="">
<div class="">On Jan 31, 2022, at 12:13 PM, Wu, Xiao Chu <<a href="mailto:xiw138@mail.usask.ca" class="">xiw138@mail.usask.ca</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class=""><font size="2" style="font-family: Helvetica; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none;" class=""><b class="">***CAUTION:***
 This email is not from a BCM Source. Only click links or open attachments you know are safe.</b></font><span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class=""></span>
<hr style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">
<div class="WordSection1" style="page: WordSection1; caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;">
<div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<span lang="EN-GB" class=""><o:p class=""> </o:p></span></div>
<div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
Dear All,<o:p class=""></o:p></div>
<div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
I have some TEM small particle images that need software process to make them more clear to see the detail. I know that SPIDER can do this process, but we are not able to install this software on a windows computer. Any one knows any other software that similar
 to SPIDER which can be installed on windows computer to process the TEM small particle images.<o:p class=""></o:p></div>
<div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
<o:p class=""> </o:p></div>
<div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
Thanks<o:p class=""></o:p></div>
<div style="margin: 0in; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
Wu<o:p class=""></o:p></div>
</div>
<span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class="">_______________________________________________</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">
<span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class="">3dem
 mailing list</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">
<a href="mailto:3dem@ncmir.ucsd.edu" style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">3dem@ncmir.ucsd.edu</a><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.ncmir.ucsd.edu_mailman_listinfo_3dem&d=DwICAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=scEf3eu1IG8eIe8pKVurLSPrFJg8M0hSTxVc2uOguBAQfq0wAZgBX1SxxZCp28Q-&s=mu3EZqxqmfegAV8feunwH4KbvxYicPeu9-M8MEVpj2A&e=" style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">https://urldefense.proofpoint.com/v2/url?u=https-3A__mail.ncmir.ucsd.edu_mailman_listinfo_3dem&d=DwICAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=scEf3eu1IG8eIe8pKVurLSPrFJg8M0hSTxVc2uOguBAQfq0wAZgBX1SxxZCp28Q-&s=mu3EZqxqmfegAV8feunwH4KbvxYicPeu9-M8MEVpj2A&e=</a><span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class=""></span></div>
</blockquote>
</div>
<br class="">
</div>
</div>
</div>
</blockquote>
</div>
<br class="">
</div>
</body>
</html>