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<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">In my experience, this should work o.k. as long as your initial angular sampling is such that the algorithm will move to finer sampling twice (or more), making sure that the refinement
“settles in” properly. That said, I rarely do that, and if I do, I use slightly lower initial resolution (6 Å).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">Good luck,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US">Basil<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;mso-fareast-language:EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><b><span style="font-size:12.0pt;color:black">From:
</span></b><span style="font-size:12.0pt;color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Jay Rai <jrai@fsu.edu><br>
<b>Date: </b>Sunday, 9 January 2022 at 02:31<br>
<b>To: </b>3dem <3dem-bounces@ncmir.ucsd.edu>, 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] Refining polished particles<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">In addition <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">The 4 angstroms reference map is unfiltered half maps on relion 4.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">With kind regards,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Jay<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;color:black">From:</span></b><span style="font-size:11.0pt;color:black"> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Jay Rai <jrai@fsu.edu><br>
<b>Sent:</b> Saturday, January 8, 2022 9:29:39 PM<br>
<b>To:</b> 3dem <3dem-bounces@ncmir.ucsd.edu>; 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [3dem] Refining polished particles</span><span style="font-size:11.0pt">
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"> <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Dear all,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Can we use 4 angstroms reference map during refinement of polish particles? Also can we also do only local alignment/refinement only if the particles before polishing yield 2.5 angstroms ( with each unfiltered
half maps are 3.5 angstroms).<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Do you think it will be a model bias? <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Any suggestions or thought are appreciated.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">With kind regards,<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Jay<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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