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<p>CDS movies contain heavy noises on the first frame. This problem
has not been solved even after very recent updates in some Krios
in Japan. But the attached images imply wrong gain-correction.<br>
<br>
Have you checked that the path to the gain reference file is
correct? After recent update on GMS, the file name of the
gain-reference was slightly changed. If you are using SerialEM,
you have to update the path to the gain-reference, or you can
simply make a symbolic link to the correct gain file.</p>
<p>Did you ever try making gain-file from the data (by using
relion_estimate_gain)?<br>
If it works, internal setting for the gain reference is somewhat
wrong.<br>
</p>
<p>Best,</p>
<p>Tomohiro</p>
<p><br>
</p>
<div class="moz-cite-prefix">On 2021/12/18 4:07, Matthijn VOS wrote:<br>
</div>
<blockquote type="cite" cite="mid:B7D72896-C5C2-4570-886D-7C9F0CAF1225@pasteur.fr">
<xmp>
</xmp>
<div dir="ltr">Exactly the same experience at Institut Pasteur.
Took more than a year, then GMS upgrade, gatan engineer playing
with the settings and gain recorded not at the dose rate
requested by the procedure, but a bit higher.</div>
<div dir="ltr"><br>
</div>
<div dir="ltr">No explanations from gatan as were the origin of
this all comes from. To this day not very satisfied and working
on replacing it with a TF filter and camera.</div>
<div dir="ltr"><br>
</div>
<div dir="ltr">Happy holidays!</div>
<div dir="ltr"><br>
<blockquote type="cite">On Dec 17, 2021, at 13:06, Wim Hagen
<a class="moz-txt-link-rfc2396E" href="mailto:hagen@embl.de"><hagen@embl.de></a> wrote:<br>
<br>
</blockquote>
</div>
<blockquote type="cite">
<div dir="ltr">
<div class="BodyFragment"><font size="2"><span style="font-size:10pt;">
<div class="PlainText"><br>
Hi Dan,<br>
<br>
Our K3 has/had this exact problem from the very start
when it was installed. For SPA one can somewhat work
around the problem, but it’s a killer for tomography.
Gatan played a bit with the detection settings for CDS
mode (which required a GMS upgrade), that somewhat
fixed the gain ref symptoms, but a subsequent ApoF SPA
test of normal vs CDS mode showed zero difference in
performance. We kinda left it there and only use
normal mode on that system.<br>
<br>
Hearsay:<br>
- Some systems have this in normal mode, or CDS mode
or both modes.<br>
-Some systems were fine after swapping the chip.<br>
- One system was fine after swapping the K3 power
supply.<br>
<br>
Best,<br>
<br>
Wim Hagen<br>
EMBL Heidelberg<br>
<br>
<br>
> On 17. Dec 2021, at 02:44, Daniel Toso
<a class="moz-txt-link-rfc2396E" href="mailto:dtoso@berkeley.edu"><dtoso@berkeley.edu></a> wrote:<br>
> <br>
> Hello Everyone, <br>
> <br>
> We have been having an issue with one of our
older K3 cameras since last year. Namely, in CDS mode
we are getting stripes in our images that do not get
removed by gain correction and become worse as the
dose goes further from 7.5 e-/pixel/sec., which will
occur quite often in thicker ice. See attached image
for an example.<br>
> <br>
> We tried having Gatan (Ametek) fix the issue but
they do not have a solution beyond buying a new
sensor.<br>
> <br>
> I was wondering if this is a widespread issue.
Also, if there is any solution besides no longer
imaging in CDS mode. We have heard that it is also
seen at UCSF. Any advice would be appreciated.<br>
> <br>
> Thanks very much!<br>
> <br>
> Best regards,<br>
> <br>
> Dan<br>
</div>
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