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Hello,</div>
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I have a few questions about Autopicking in Relion.</div>
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I would like to have a more reasoned approach for choosing --min_avg_noise and --max_avg_noise.</div>
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is there a calculation somewhere of these values for either single or groups of micrographs?</div>
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more fundamentally, can I see a histogram of intensities of my images?</div>
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Any other details on find parameters for autopicking to pick filaments rather than background (which it is doing exclusively on my new dataset; see attached) would be appreciated -- and yes, the regions below the green circles are background; not a single filament
segment picked.</div>
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In Relion 3.1.1, I could see the results of autopicking with the command (e.g.):</div>
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<span>`which relion_manualpick` --i Select/job011/micrographs_split1.star --odir AutoPick/job002/ --pickname autpic --allow_save --selection Select/job003/micrographs_selected.star --scale 0.2 --sigma_contrast 3 --black 0 --white 0 --lowpass 20 --ctf_scale
1 --particle_diameter 100 </span><br>
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<span>but I cannot find anything equivalent in Relion 4.0. Is it somewhere else?</span></div>
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<span>thanks very much.</span></div>
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<span>Sincerely,</span></div>
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<span>Esther<img></span></div>
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