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<p class="MsoNormal">Hello all<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="EN-US">I wonder if anyone here has experienced issues with resin infiltration/polymerization in the pigmented epithelium in mouse retina, the pigment granules to be more specific. We have been really struggling with this. The
samples are fixed with Karnovsky fix (2% FA, 2.5 GA in cacodylate buffer). We have tried all sorts of combinations including microwave-assisted dehydration and resin infiltration in the Leica processor, switching to propylene oxide for 3 extra final solvent
changes, Hard-Plus, Durcupan and LR White resins. The resin is consistently separating from the periphery of pigment granules and in many places this adds up to severe cracking of the sections. Please do let me know if you have managed to optimise something
similar or have any suggestions. <o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">All the best,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">Anna<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">TEM:<o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:SV"><img width="569" height="591" style="width:5.925in;height:6.1583in" id="Picture_x0020_2" src="cid:image002.jpg@01D756CC.C4ACE020"></span><span lang="EN-US"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-fareast-language:SV"><img width="582" height="597" style="width:6.0666in;height:6.2166in" id="Picture_x0020_3" src="cid:image006.jpg@01D756CC.C4ACE020"></span><span lang="EN-US"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US">SEM:<o:p></o:p></span></p>
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