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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">After stitching there is no way to determine the resolution – you have introduced sharp boundaries that will lead to incorrect estimation of resolution. Why
not simply report the resolutions of the individual maps that you have used to produce the chimeric structure?<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">--Joachim<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu]
<b>On Behalf Of </b>Jay Rai<br>
<b>Sent:</b> Thursday, May 20, 2021 1:59 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu; 3dem<br>
<b>Subject:</b> [EXTERNAL] [3dem] About over-estimation<o:p></o:p></span></p>
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<p class="MsoNormal">Dear Cryo EM folks, <o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:14.0pt;color:black">I am just curious..<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:14.0pt;color:black">I focus refine each subunit of maps seperately. Then I used chimera's vop maximum to stich the map.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:14.0pt;color:black">But when I refine that map to model, it is showing my resolution 2 angstrom.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:14.0pt;color:black">I even take the unfiltered map from each subunits and stich and so the relion_preprocess and again same resolution.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:14.0pt;color:black">I know it is because of the noise from each maps (which are outside the mask). I stick the maps from multi body refinement and it is ok so it must be the noise that makes the resolution higher.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:14.0pt;color:black">Is there any way to calculate the resolution? Or in other words, is there any way to create the composite map?
<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:14.0pt;color:black">Any help is greatly appreciated.<o:p></o:p></span></p>
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<p style="background:white"><span style="font-size:14.0pt;color:black">With kind regards,</span><span style="font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
<p style="background:white"><span style="font-size:14.0pt;color:black">Jay Rai</span><span style="font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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