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Hello,
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<div class="">This page of the RELION wiki has some relevant info under sections "Coordinate system" and "Symmetry": <a href="https://urldefense.com/v3/__https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Conventions_&_File_formats__;!!Mih3wA!TJpGpHo4JZAc7MEONemLOB1cU6eFXtMfCOw5p1u9-lxwLtApjHRwFbLMXwbQck6Xqw$" class="">https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Conventions_%26_File_formats</a></div>
<div class="">I have not used other programs as much, so I have not yet run into this problem with different conventions. I hope this is still helpful to you.<br class="">
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<div class="">Cheers,</div>
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Guillaume</div>
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<div class="">On 3 Feb 2021, at 23:52, Rodarte, Justas V <<a href="mailto:jrodarte@fredhutch.org" class="">jrodarte@fredhutch.org</a>> wrote:</div>
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<span style="font-size: 11pt;" class="">Hi all,<o:p class=""></o:p></span></div>
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<span style="font-size: 11pt;" class=""><o:p class=""> </o:p></span></div>
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<span style="font-size: 11pt;" class="">I’m a relative newcomer to the field of Cryo-EM, so my apologies if this question is easy/misplaced/previously answered. I’m working on a VLP with I symmetry that displays 80 spikes. Some programs (Relion, cisTEM) apply
symmetry correctly when moving onto 3D ab-initio, while others (Cryosparc), don’t. I was able to trace this to the fact that these programs use different matrices to apply symmetry, an example is included below. Does anyone know why this is though? To me it
appears to be an oversight that two popular programs apply the same symmetry differently?<o:p class=""></o:p></span></div>
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<span style="font-size: 11pt;" class=""><o:p class=""> </o:p></span></div>
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<span style="font-size: 11pt;" class="">Thank you all for any help or advice you can provide!<o:p class=""></o:p></span></div>
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<span style="font-size: 11pt;" class=""><o:p class=""> </o:p></span></div>
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<span style="font-size: 11pt;" class="">Best,<o:p class=""></o:p></span></div>
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<span style="font-size: 11pt;" class="">Justas Rodarte<o:p class=""></o:p></span></div>
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<span style="font-size: 11pt;" class=""><o:p class=""> </o:p></span></div>
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<span style="font-size: 11pt;" class=""><o:p class=""> </o:p></span></div>
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I1 Sym Matrix Relion<o:p class=""></o:p></div>
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rot_axis 2, 1 0 0<o:p class=""></o:p></div>
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rot_axis 5, 0.85065080702670 0 -0.5257311142635<o:p class=""></o:p></p>
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rot_axis 3, 0.9341723640 0.3568220765 0<o:p class=""></o:p></div>
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<o:p class=""> </o:p></div>
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I1 Sym Matrix Cryosparc<o:p class=""></o:p></div>
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rot_axis 2, 0 1 0<o:p class=""></o:p></div>
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rot_axis 5, -0.85065080702670 0 0.5257311142635<o:p class=""></o:p></div>
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rot_axis 3, -0.9341723640 0.3568220765<o:p class=""></o:p></div>
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<span style="font-size: 11pt;" class=""><o:p class=""> </o:p></span></div>
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<span style="font-size: 11pt;" class=""><o:p class=""> </o:p></span></div>
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