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<span lang="EN-US">Hi all,<o:p></o:p></span></p>
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<span lang="EN-US"> </span></p>
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<span lang="EN-US">We have positions open for a PhD student and PostDoc to work on super-resolution light microscopy correlated with cryoEM (super-res cryoCLEM).<o:p></o:p></span></p>
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<span lang="EN-US"><i>The projects</i>: We've developed a method to perform super-res cryoCLEM on intact mammalian cells using fluorescent proteins (Tuijtel et al, Sci Rep 2019:
</span><a href="https://urldefense.com/v3/__https://www.nature.com/articles/s41598-018-37728-8__;!!Mih3wA!SGO3du_MwJtTBPwIKWfgKOOjbwX1Th0xaekAPwGsYaOBWtQQdZ9rVGHT5r7jRfuTfg$" style="color: rgb(149, 79, 114);" id="LPlnk196465" previewremoved="true"><span lang="EN-US">https://www.nature.com/articles/s41598-018-37728-8</span></a><span lang="EN-US">). We’re now at the
stage where we can use this technique to investigate cellular processes, with a focus on the human immune system. We are also developing the equipment and techniques further, to improve ease of use and explore novel methods for super-res cryoCLEM.<o:p></o:p></span></p>
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<span lang="EN-US"><i>Profiles</i>: The positions are flexible, and range from developing new microscopy techniques to applying super-res cryoCLEM to biological specimens.<o:p></o:p></span></p>
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<span lang="EN-US">Postdocs are expected to have a solid background in super-resolution LM or cryoEM.<o:p></o:p></span></p>
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<span lang="EN-US">For PhD applicants, a background in microscopy/physics/engineering would be highly advantageous, but not essential. Alternatively, you have experience with biology/immunology with an evident desire to explore the fundamentals of super-res
cryoCLEM.<o:p></o:p></span></p>
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<span lang="EN-US"><i>The lab</i>: We are at the Leiden University Medical Centre (LUMC), in the Netherlands, which is a well-funded lab in a great place to live and work. We are a highly interdisciplinary group, ranging from bionanotechnology, structural biology,
to immunology. We routinely collaborate with chemists, biologists, medics and microscopists.<o:p></o:p></span></p>
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<span lang="EN-US">If you are interested, please send me a CV and cover letter. I’m also very happy to receive informal questions about the position, project, or living in Leiden. There is currently no deadline but we will close the positions as soon as they
are filled.<o:p></o:p></span></p>
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<span lang="EN-US">All the best,<o:p></o:p></span></p>
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Thom</p>
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<span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif"></span><span style="color:rgb(54,95,145); font-size:10pt; font-family:Arial,Helvetica,sans-serif"><br>
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<span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif"></span><b><span style="color:rgb(54,95,145); font-size:10pt; font-family:Arial,Helvetica,sans-serif"><span style="font-family:Arial,Helvetica,sans-serif">Dr. Thomas H. Sharp</span><span style="font-family:Arial,Helvetica,sans-serif"></span><u></u><u></u></span></b></p>
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<span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif"></span><span style="color:gray"><font size="2"><span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif">Assistant Professor</span><u></u><u></u></font></span></p>
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<span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif"></span><span style="color:gray"><font size="2"><span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif">PI: Bio-Nanopatterning</span><font face="Arial, Helvetica, sans-serif"><u></u><u></u></font></font></span></p>
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<span style="font-size:10pt"></span><font size="2"><span lang="NL" style="color:gray; font-family:Wingdings; font-size:10pt">(</span><span style="color:gray"><font face="Arial, Helvetica, sans-serif"><span style="font-size:10pt"> </span></font><span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif">0031-(0)-7152-69499</span><font face="Arial, Helvetica, sans-serif"><u></u><u></u></font></span></font></p>
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<span style="font-size:10pt"></span><font size="2"><span lang="NL" style="color:gray; font-family:Wingdings; font-size:10pt">*</span><span lang="NL" style="color:gray; font-family:Arial,Helvetica,sans-serif; font-size:10pt"> </span><span style="color:gray"><a href="mailto:t.sharp@lumc.nl" target="_blank" id="LPNoLP" style="color:rgb(17,85,204)"><span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif">t.sharp@lumc.nl</span></a></span></font></p>
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<span style="font-size:10pt"></span><span style="color:gray"><span><span style="color:rgb(128,128,128); font-family:Tahoma; font-size:13px"><span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif">Website: </span><a href="https://urldefense.com/v3/__https://ccb.lumc.nl/about-the-sharp-lab-90__;!!Mih3wA!SGO3du_MwJtTBPwIKWfgKOOjbwX1Th0xaekAPwGsYaOBWtQQdZ9rVGHT5r5FQ6cc2A$" class="OWAAutoLink" id="LPNoLP"><span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif">https://ccb.lumc.nl/about-the-sharp-lab-90</span></a></span><br>
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<span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif"></span><span style="color:gray"><span style="font-size:10pt; font-family:Arial,Helvetica,sans-serif">Visiting address: LUMC Bldg. 2, Room R-90-10, Einthovenweg 20, 2333 ZC Leiden</span><br>
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