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Dear Linda,
<div class="">We have used fixation for correlative LM-cryoEM experiments (for example <a href="https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/20639870/__;!!Mih3wA!SJwd1NqTxx1bXMjossuohJxrU9Q7mCM-qQ4lLyqR9ErDPMYp2CBir3NwZpu9_H3NYw$" class="">https://pubmed.ncbi.nlm.nih.gov/20639870/</a>)</div>
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<div class="">or for BSL 3 samples (Published <a href="https://urldefense.com/v3/__https://www.pnas.org/content/112/28/E370__;!!Mih3wA!SJwd1NqTxx1bXMjossuohJxrU9Q7mCM-qQ4lLyqR9ErDPMYp2CBir3NwZpuw6vtnLg$" class="">https://www.pnas.org/content/112/28/E370</a>, <a href="https://urldefense.com/v3/__https://jb.asm.org/content/201/12/e00134-19__;!!Mih3wA!SJwd1NqTxx1bXMjossuohJxrU9Q7mCM-qQ4lLyqR9ErDPMYp2CBir3NwZps3hHjefg$" class="">https://jb.asm.org/content/201/12/e00134-19</a>, <a href="https://urldefense.com/v3/__https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417958/__;!!Mih3wA!SJwd1NqTxx1bXMjossuohJxrU9Q7mCM-qQ4lLyqR9ErDPMYp2CBir3NwZpuX5IAN0w$" class="">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417958/</a>)</div>
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<div data-pm-slice="1 1 []" data-en-clipboard="true" class="">I hope this is useful. DM me if you have more specific questions. </div>
<div data-pm-slice="1 1 []" data-en-clipboard="true" class="">All the best, </div>
<div data-pm-slice="1 1 []" data-en-clipboard="true" class="">Ariane </div>
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<div>––––––––––––––––––––––––––––<br class="">
Ariane Briegel<br class="">
Professor, Co-Director NeCEN<br class="">
Leiden University<br class="">
Sylvius Laboratorium<br class="">
Sylviusweg 72<br class="">
2333 BE Leiden<br class="">
T: 071-527 8850<br class="">
<a href="mailto:a.briegel@biology.leidenuniv.nl" class="">a.briegel@biology.leidenuniv.nl</a><br class="">
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<div class="">On 18 Jan 2021, at 12:18, Linda Sandblad <<a href="mailto:linda.sandblad@umu.se" class="">linda.sandblad@umu.se</a>> wrote:</div>
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Dear best colleagues!
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<div class="">What chemical fixation protocols works for your projects before plunge freezing (or HPF) or what typs of chemical fixation was not good (destroyed more than it helped) prior to cryo-EM sample preparation?</div>
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<div class="">Im interested in your experiences, also happy if you have adressed this question in a article and would like to share the link with me.</div>
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<div class="">For cryo-EM we prefer to work as close to native as possible, avoiding chemical fixations, but sometimes a fixation is needed to test or move on with tricky projects. For examples, when bacteria cultures are tricky and samples need to be stored,
or when bio safety restricts work in the EM labs (lab specific and different).</div>
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<div class="">Best regards, Linda</div>
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Linda Sandblad, researcher and facility director<br class="">
Umeå Core Facility for Electron Microscopy, SciLifeLab Cryo-EM, NMI<br class="">
Department of Chemistry, Umeå University, Sweden<br class="">
Tel: +46 709324936</div>
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