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<p>Dear Linda,</p>
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<p>When working with cells (eukaryote or prokaryote) that need fixation because of safety issues, I usually fix them in 4% PFA.</p>
<p>This protocol never gave broken/damaged cells.</p>
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<p>On what type of sample do you want to use chemical fixation ?</p>
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<p>Kind regards,</p>
<p>Sylvain</p>
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Sylvain TREPOUT, PhD, HDR</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>De :</b> 3dem <3dem-bounces@ncmir.ucsd.edu> de la part de Linda Sandblad <linda.sandblad@umu.se><br>
<b>Envoyé :</b> lundi 18 janvier 2021 12:18:42<br>
<b>À :</b> 3dem@ncmir.ucsd.edu<br>
<b>Objet :</b> [3dem] Chemical fixation before plunge freezing or HPF</font>
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<div>Dear best colleagues!
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<div class="">What chemical fixation protocols works for your projects before plunge freezing (or HPF) or what typs of chemical fixation was not good (destroyed more than it helped) prior to cryo-EM sample preparation?</div>
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<div class="">Im interested in your experiences, also happy if you have adressed this question in a article and would like to share the link with me.</div>
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<div class="">For cryo-EM we prefer to work as close to native as possible, avoiding chemical fixations, but sometimes a fixation is needed to test or move on with tricky projects. For examples, when bacteria cultures are tricky and samples need to be stored,
or when bio safety restricts work in the EM labs (lab specific and different).</div>
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<div class="">Best regards, Linda</div>
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Linda Sandblad, researcher and facility director<br class="">
Umeå Core Facility for Electron Microscopy, SciLifeLab Cryo-EM, NMI<br class="">
Department of Chemistry, Umeå University, Sweden<br class="">
Tel: +46 709324936</div>
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