<html xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<style><!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Calibri",sans-serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:#0563C1;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:#954F72;
text-decoration:underline;}
span.EmailStyle17
{mso-style-type:personal-compose;
font-family:"Calibri",sans-serif;
color:windowtext;}
.MsoChpDefault
{mso-style-type:export-only;
font-family:"Calibri",sans-serif;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style>
</head>
<body lang="EN-US" link="#0563C1" vlink="#954F72">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-size:11.0pt">Hello 3demers,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Does anyone have any experience using/owing a low-voltage “desktop” TEM? I see there are some 25 keV ones available and they seem to work fine for large negatively stained samples such as viruses and phages.
But, I am wondering how successful they are for small protein complexes or membrane proteins?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Any user feedback would be greatly appreciated.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Thanks!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Kristin <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif">-- <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif">Sew4science mask project info
<a href="https://urldefense.com/v3/__https://sew4science.onuniverse.com/__;!!Mih3wA!UTh-qcbxt2GQ3OGri4QWuG3bEkJ1AYWgcfdq0jUAzKKSYlwt2HgRymsO4-WQ8RDp_Q$"><span style="color:#0563C1">here</span></a>! Mask and stay safe.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<div>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Kristin N. Parent, PhD (she/her)<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">JK Billman, Jr., MD Endowed Research Professor<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Director, MSU Cryo-EM<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Associate Professor of Biochemistry and Molecular Biology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Michigan State University<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">603 Wilson Rd<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">East Lansing, MI 48824<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Phone: 517-432-8434<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black"><a href="https://urldefense.com/v3/__https://kparentlab.natsci.msu.edu/__;!!Mih3wA!UTh-qcbxt2GQ3OGri4QWuG3bEkJ1AYWgcfdq0jUAzKKSYlwt2HgRymsO4-U32iA84g$"><span style="color:#954F72">https://kparentlab.natsci.msu.edu/</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Twitter: @Phage4Lyfe<o:p></o:p></span></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
</body>
</html>