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<p class="MsoNormal">Hello Interested Researchers,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Just a reminder that this webinar is occurring this Thursday 10/29 at 12PM EDT. This is our second NIH sponsored Joint CryoEM Service Centers Webinar (details below or attached).<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">If you still haven’t registered, there is still time: <span lang="DE">
<a href="https://urldefense.com/v3/__https://gcc02.safelinks.protection.outlook.com/?url=https*3A*2F*2Fus02web.zoom.us*2Fwebinar*2Fregister*2FWN_Q3_zFm98R06EPHKF7ZRlCA&data=02*7C01*7Clauren.halesbeck*40pnnl.gov*7C80c5a98d18db414f5a8c08d863eb7f6c*7Cd6faa5f90ae240338c0130048a38deeb*7C0*7C1*7C637369210397959687&sdata=uXNv7YFDaBe1e8oezQcX1qaBw5Bh0PX4Imt*2BDnUP1KE*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!V7IVc9fzhPPDjiefndgvugrL_umPPaotfJ_vbMeOrgi8ZvaKodbPGDWWES_ttfYarw$">https://us02web.zoom.us/webinar/register/WN_Q3_zFm98R06EPHKF7ZRlCA</a></span>
.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" style="margin-bottom:12.0pt">Best,<br>
<b><span style="color:#E08A32">_________________________<br>
</span></b><b><span style="font-size:13.0pt">Lauren B. Hales Beck<br>
</span></b><span style="font-size:9.0pt">Project Coordinator</span><b><span style="font-size:8.0pt;color:#E08A32"><o:p></o:p></span></b></p>
<p class="MsoNormal"><b>Pacific Northwest Cryo-EM Center<br>
</b><span style="font-size:8.0pt">OHSU, Oregon Health and Science University<br>
RLSB, Robertson Life and Science Building<br>
2730 S. Moody Ave CL-P2M. Portland, OR 97201-5042<br>
</span><span style="font-size:9.0pt">a <b>PNNL</b> and <b>
OHSU</b> affiliation</span><span style="font-size:8.0pt"> </span><a href="https://urldefense.com/v3/__https://pncc.labworks.org/__;!!Mih3wA!V7IVc9fzhPPDjiefndgvugrL_umPPaotfJ_vbMeOrgi8ZvaKodbPGDWWES-XFxMI2w$"><span style="font-size:8.0pt;color:blue">Website</span></a><span style="font-size:8.0pt">
</span><a href="https://urldefense.com/v3/__https://twitter.com/CryoEM_PNCC__;!!Mih3wA!V7IVc9fzhPPDjiefndgvugrL_umPPaotfJ_vbMeOrgi8ZvaKodbPGDWWES87vj0v8g$"><span style="font-size:8.0pt;color:blue">PNCC Twitter</span></a><span style="font-size:9.0pt"><br>
</span><span style="font-size:8.0pt;color:#538135"><br>
</span><span style="font-size:9.0pt">Phone: 509.372.4382<br>
Email: </span><a href="mailto:lauren.halesbeck@pnnl.gov"><span style="font-size:9.0pt;color:blue">lauren.halesbeck@pnnl.gov</span></a><o:p></o:p></p>
<p class="MsoNormal"><b><o:p> </o:p></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><a href="https://urldefense.com/v3/__https://nccat.nysbc.org/__;!!Mih3wA!V7IVc9fzhPPDjiefndgvugrL_umPPaotfJ_vbMeOrgi8ZvaKodbPGDWWES9OUAWf3Q$"><span style="color:windowtext;text-decoration:none"><img border="0" width="114" height="114" style="width:1.1875in;height:1.1875in" id="Picture_x0020_6" src="cid:image002.png@01D6AC7D.C5236910" alt="A close up of a sign
Description automatically generated"></span></a><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">
</span><a href="https://urldefense.com/v3/__https://cryoem.slac.stanford.edu/s2c2/__;!!Mih3wA!V7IVc9fzhPPDjiefndgvugrL_umPPaotfJ_vbMeOrgi8ZvaKodbPGDWWES_jZH7v1g$"><span style="color:windowtext;text-decoration:none"><img border="0" width="265" height="58" style="width:2.7583in;height:.6041in" id="Picture_x0020_3" src="cid:image004.png@01D6AC7D.C5236910" alt="A picture containing drawing
Description automatically generated"></span></a><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"> </span><a href="https://urldefense.com/v3/__https://pncc.labworks.org/__;!!Mih3wA!V7IVc9fzhPPDjiefndgvugrL_umPPaotfJ_vbMeOrgi8ZvaKodbPGDWWES-XFxMI2w$"><span style="color:windowtext;text-decoration:none"><img border="0" width="160" height="78" style="width:1.6666in;height:.8125in" id="Graphic_x0020_5" src="cid:image006.png@01D6AC7D.C5236910"></span></a><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="DE" style="font-size:12.0pt;font-family:"Cambria",serif;mso-fareast-language:DE"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="DE" style="font-size:12.0pt;font-family:"Cambria",serif;mso-fareast-language:DE"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="DE" style="font-size:12.0pt;font-family:"Cambria",serif;mso-fareast-language:DE"><a href="https://urldefense.com/v3/__https://us02web.zoom.us/webinar/register/WN_Q3_zFm98R06EPHKF7ZRlCA__;!!Mih3wA!V7IVc9fzhPPDjiefndgvugrL_umPPaotfJ_vbMeOrgi8ZvaKodbPGDWWES9UZotyeg$"><b><span lang="EN-US" style="font-size:20.0pt;font-family:"Arial",sans-serif;color:blue;mso-fareast-language:EN-US">Webinar</span></b></a></span><b><u><span style="font-size:20.0pt;font-family:"Arial",sans-serif;color:#385623"><o:p></o:p></span></u></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-size:12.0pt;font-family:"Arial",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><i><span style="font-size:20.0pt;font-family:"Arial",sans-serif;color:#5B9BD5">Sample preparation vs. data collection conditions for high-resolution CryoEM structures of NMDARs<o:p></o:p></span></i></b></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" align="center" style="text-align:center"><img border="0" width="338" height="270" style="width:3.5208in;height:2.8125in" id="Picture_x0020_2" src="cid:image008.jpg@01D6AC7D.C5236910"><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><i><span style="font-size:18.0pt;font-family:"Arial",sans-serif;color:#ED7D31;mso-fareast-language:DE">Dr. Farzad Jalali-Yazdi <o:p></o:p></span></i></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-family:"Arial",sans-serif;mso-fareast-language:DE">PostDoctoral Fellow<o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-family:"Arial",sans-serif;mso-fareast-language:DE">Oregon Health and Science University<o:p></o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-family:"Arial",sans-serif;mso-fareast-language:DE"><o:p> </o:p></span></b></p>
<p class="MsoNormal" align="center" style="text-align:center"><b><span style="font-family:"Arial",sans-serif;color:black;mso-fareast-language:DE">12PM EDT / 9AM PDT Thursday, October 29th, 2020<o:p></o:p></span></b></p>
<p class="MsoNormal" style="text-align:justify"><span style="font-family:"Times New Roman",serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal" style="text-align:justify"><span style="font-family:"Arial",sans-serif;color:black">NMDA receptors are ion channels that help mediate the fast excitatory neurotransmission in the central nervous system. The first structures of intact NMDA
receptors were solved by crystallography in 2014, and the first cryoEM structures of the receptor were solved in 2016, though at relatively low resolution. Since then we have been trying to improve the resolution of the CryoEM structures, especially in the
transmembrane domain of the receptor. To do this, we have used a variety of data collection and processing strategies, construct optimization, sample purification, and additive screening. In this talk, I will go through the strategies we have used, and the
recent results we have obtained with significantly improved resolution, as well as some pitfalls that are easy to fall into.<o:p></o:p></span></p>
<p class="MsoNormal" style="text-align:justify"><span style="font-family:"Arial",sans-serif;color:black"><o:p> </o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span style="font-family:"Arial",sans-serif;color:black">All are welcome to attend. Registration is at no-cost, but sign-up is required:<o:p></o:p></span></p>
<p class="MsoNormal" align="center" style="text-align:center"><span lang="DE" style="font-size:12.0pt;font-family:"Cambria",serif;mso-fareast-language:DE"><a href="https://urldefense.com/v3/__https://gcc02.safelinks.protection.outlook.com/?url=https*3A*2F*2Fus02web.zoom.us*2Fwebinar*2Fregister*2FWN_Q3_zFm98R06EPHKF7ZRlCA&data=02*7C01*7Clauren.halesbeck*40pnnl.gov*7C80c5a98d18db414f5a8c08d863eb7f6c*7Cd6faa5f90ae240338c0130048a38deeb*7C0*7C1*7C637369210397959687&sdata=uXNv7YFDaBe1e8oezQcX1qaBw5Bh0PX4Imt*2BDnUP1KE*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!V7IVc9fzhPPDjiefndgvugrL_umPPaotfJ_vbMeOrgi8ZvaKodbPGDWWES_ttfYarw$"><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:blue">https://us02web.zoom.us/webinar/register/WN_Q3_zFm98R06EPHKF7ZRlCA</span></a></span><span style="font-family:"Arial",sans-serif;color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">This webinar series is jointly hosted by the</span>
<span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">NIH Transformative High Resolution CryoEM Program Service Centers: the National Center for CryoEM Access and Training (NCCAT), the Pacific Northwest Center for CryoEM (PNCC), and the
Stanford-SLAC CryoEM Center (S2C2) who provide no-cost access to cryoEM instrumentation and training. In this monthly series, we will highlight cryoEM methods and use the Q&A session after the seminar to stimulate discussion of best practices and interesting
challenges that will be helpful to researchers new to the field. Representatives from all three service centers will also be on hand to answer questions about the cryoEM resources available to biomedical researchers and how to access them.</span><span style="font-size:10.0pt;font-family:"Times New Roman",serif"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:8.0pt;color:#538135"><o:p> </o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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