<div dir="auto">Has this been rolled out for all the cryoem EM facility World wide? How many facilities has been asked to fill out BSL questionnaire from TFS. Our local service refuse to share the information with us while want to jacking up the price. </div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Mon, 6 Jul 2020, 13:52 Matthijn VOS, <<a href="mailto:matthijn.vos@pasteur.fr">matthijn.vos@pasteur.fr</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
<div dir="auto">
<div dir="ltr">Maybe this helps</div>
<div dir="ltr"><br>
</div>
<div dir="ltr"><a href="https://urldefense.com/v3/__https://consteril.com/biosafety-levels-difference/__;!!Mih3wA!RuMslxLdN3kR1D691b73ibuiBpdRTB4Pj7CJfGpPAKBAWCOWXQRf9HrT2nCmvoRzaQ$" target="_blank" rel="noreferrer">https://consteril.com/biosafety-levels-difference/</a></div>
<div dir="ltr"><br>
</div>
<div dir="ltr">The difference in lab setup between BSL 1 and 2 are not that big. A lab manager can also decide to declare a lab BSL2 for a period of time, then decontaminate and resume operation as BSL1. TFS only charges extra service cost if microscope decontamination
procedures are involved and parts can not be taken back. In BSL3 all parts stay in the facility until the lab closes. This means a company can not recover parts and reuse them. This will increase cost. Also decontamination procedures might damage certain parts
that need to be replaced (other than the broken parts that needed fixing), which again increases cost. For BSL2 this does all not apply. Wearing lab coats, gloves, presence of a sink, ways to dispose of infectious material, a safety cabinet and modifications
to the air flow I believe are required but are all “reversible” back to BSL1. There are no specifications based on disinfecting the inside of the microscope. Best is to contact your local TFS agent, they have a full unit for dealing with this, there are many
BSL2 facilities worldwide that house EM.</div>
<div dir="ltr"><br>
</div>
<div dir="ltr">Cheers</div>
<div dir="ltr"><br>
<blockquote type="cite">On Jul 6, 2020, at 04:21, jamon <<a href="mailto:jamonshi@gmail.com" target="_blank" rel="noreferrer">jamonshi@gmail.com</a>> wrote:<br>
<br>
</blockquote>
</div>
<blockquote type="cite">
<div dir="ltr">
<p>I heard there will be ~10% increase of service contract from BSL1 to BSL2. Has it been applied world wide?
<br>
</p>
<p>Regards,</p>
<p>Jian<br>
</p>
<div>On 6/7/20 9:21 am, Sherman, Michael B. wrote:<br>
</div>
<blockquote type="cite">
<div>
<p class="MsoNormal">Hi Ruben,<u></u><u></u></p>
<p class="MsoNormal">Jaap is correct, BSL2 assumes standard lab practice, lab coat, gloves at the most, but no special safety/decon procedures. And no service cost increase. TFS does not want to deal with BSL3, so that is out of question. Our Krios is classified
by TFS as level 2 instrument and my engineers come and go as they like (face mask is required currently… for a different reason). I do not see any cosequences declaring your microscope s BSL2.<u></u><u></u></p>
<p class="MsoNormal">All the best,<u></u><u></u></p>
<p class="MsoNormal">Misha<u></u><u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal"><span style="color:black">Michael Sherman, PhD<br>
Dept. of Biochem. & Mol. Biology<br>
1.224 Medical Research Building<br>
Univ. of Texas Medical Branch<br>
Galveston, TX 77555-1055<br>
Tel. 409-772-6310<br>
Fax: 409-772-6299<br>
</span><a href="mailto:mbsherma@UTMB.edu" target="_blank" rel="noreferrer"><span style="color:purple">mbsherma@UTMB.edu</span></a><u></u><u></u></p>
</div>
<p class="MsoNormal"><u></u> <u></u></p>
<p class="MsoNormal"><u></u> <u></u></p>
<div style="border:none;border-top:solid #b5c4df 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">3dem
<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank" rel="noreferrer"><3dem-bounces@ncmir.ucsd.edu></a> on behalf of Jaap Brink
<a href="mailto:jbrink@jeol.com" target="_blank" rel="noreferrer"><jbrink@jeol.com></a><br>
<b>Date: </b>Sunday, July 5, 2020 at 8:10 PM<br>
<b>To: </b>Ruben Diaz <a href="mailto:pindusito@gmail.com" target="_blank" rel="noreferrer">
<pindusito@gmail.com></a><br>
<b>Cc: </b><a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" rel="noreferrer">"3dem@ncmir.ucsd.edu"</a>
<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" rel="noreferrer"><3dem@ncmir.ucsd.edu></a><br>
<b>Subject: </b>Re: [3dem] BSL2 vs BSL1<u></u><u></u></span></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div style="border:solid black 1.0pt;padding:2.0pt 2.0pt 2.0pt 2.0pt">
<p class="MsoNormal" style="line-height:12.0pt;background:#ffeb9c"><b><span style="font-size:10.0pt;color:black">WARNING:</span></b><span style="font-size:10.0pt;color:black"> This email originated from outside of UTMB's email system. Do not click links or
open attachments unless you recognize the sender and know the content is safe.<u></u><u></u></span></p>
</div>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<p class="MsoNormal" style="margin-bottom:12.0pt">Forgot to add that before SVC enters the facility, a wipe down is required. Akin to what you’d do with a lab bench. <u></u><u></u></p>
<div>
<div>
<p class="MsoNormal">Regards,<u></u><u></u></p>
</div>
<p class="MsoNormal">Jaap <u></u><u></u></p>
<div>
<p class="MsoNormal">---- <u></u><u></u></p>
<div>
<p class="MsoNormal">Jacob Brink <span style="font-size:13.0pt">Ph.D.</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:13.0pt">TEM Product Manager Life Sciences</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><b><span style="font-size:13.0pt">JEOL USA, Inc.,</span></b><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:13.0pt">Peabody MA 01960, U.S.A.</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:13.0pt">Cell: +1 (978) 317-9561</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Sent from a mobile device. Please excuse typos and this email’s brevity.<u></u><u></u></p>
</div>
</div>
</div>
<div>
<p class="MsoNormal"><br>
<br>
<u></u><u></u></p>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal" style="margin-bottom:12.0pt">On Jul 5, 2020, at 8:07 PM, Jaap Brink
<a href="mailto:jbrink@jeol.com" target="_blank" rel="noreferrer"><jbrink@jeol.com></a> wrote:<u></u><u></u></p>
</blockquote>
</div>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<div>
<p class="MsoNormal">Hi Ruben, <u></u><u></u></p>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal" style="margin-bottom:12.0pt">Don’t think BSL2 requires that. BSL3 for sure does and requires elaborate decon procedures to be in place. UTMB goes for a SVC visit back from level 3 to level 2. It is then safe to enter. <u></u><u></u></p>
<div>
<div>
<p class="MsoNormal">Regards,<u></u><u></u></p>
</div>
<p class="MsoNormal">Jaap <u></u><u></u></p>
<div>
<p class="MsoNormal">---- <u></u><u></u></p>
<div>
<p class="MsoNormal">Jacob Brink <span style="font-size:13.0pt">Ph.D.</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:13.0pt">TEM Product Manager Life Sciences</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><b><span style="font-size:13.0pt">JEOL USA, Inc.,</span></b><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:13.0pt">Peabody MA 01960, U.S.A.</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><span style="font-size:13.0pt">Cell: +1 (978) 317-9561</span><u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Sent from a mobile device. Please excuse typos and this email’s brevity.<u></u><u></u></p>
</div>
</div>
</div>
<div>
<p class="MsoNormal"><br>
<br>
<u></u><u></u></p>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<p class="MsoNormal" style="margin-bottom:12.0pt">On Jul 5, 2020, at 7:31 PM, Ruben Diaz
<a href="mailto:pindusito@gmail.com" target="_blank" rel="noreferrer"><pindusito@gmail.com></a> wrote:<u></u><u></u></p>
</blockquote>
</div>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<div>
<div>
<div>
<p class="MsoNormal">Hi Jian,<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Yes, if we decide to put BSL2 specimens in the microscopes, we have to tell TFS about it, to make sure that their engineers are safe.<u></u><u></u></p>
</div>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Ruben.<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
<div>
<div>
<p class="MsoNormal">On Sun, Jul 5, 2020 at 5:28 PM jamon <<a href="mailto:jamonshi@gmail.com" target="_blank" rel="noreferrer">jamonshi@gmail.com</a>> wrote:<u></u><u></u></p>
</div>
<blockquote style="border:none;border-left:solid #cccccc 1.0pt;padding:0in 0in 0in 6.0pt;margin-left:4.8pt;margin-right:0in">
<div>
<p>Dear Ruben,<u></u><u></u></p>
<p>Is it request by TFS (FEI)? <u></u><u></u></p>
<p>Regards,<u></u><u></u></p>
<p>Jian<u></u><u></u></p>
</div>
<div>
<div>
<p class="MsoNormal">On 6/7/20 7:17 am, Ruben Diaz wrote:<u></u><u></u></p>
</div>
</div>
<div>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<div>
<p class="MsoNormal">Hi all, <u></u><u></u></p>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Due to the type of samples that we are interested in imaging, it would be advantageous for us to declare our facility as BSL2. This designation, however, has some implications in terms of the paperwork and safety measures that have to be
taken whenever service is called. Cost of course I am sure is another factor. Does anyone have experience as to the real implications that have to be considered? It is my understanding that once we declare the facility to be BSL2, every instrument is included
in the designation, and it is not reversible.<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Any information will be greatly appreciated.<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">Ruben.<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
<div>
<p class="MsoNormal">***************************<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal">Ruben Diaz Avalos,<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal">La Jolla Institute for Immunology,<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><a href="https://urldefense.com/v3/__https://nam11.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.google.com*2Fmaps*2Fsearch*2F9420*Athena*Cir*2C*La*Jolla*2C*CA*92037*3Fentry*3Dgmail*26source*3Dg__*3BKysrKysr!!Mih3wA!VF5pTkhY_jgbypbTZ84Z_wII5hzOEDg6Q_xEEk1iMjZ0w-xXRhuHwYXCownxYUuv-A*24&data=02*7C01*7Cmbsherma*40utmb.edu*7C7588ba702e3d48b0331608d82149701a*7C7bef256d85db4526a72d31aea2546852*7C0*7C0*7C637295946590205739&sdata=gf0ueEnZLbDRuXn9RIx8b6Ws6KfOjt3RGkqkvrhANt4*3D&reserved=0__;JSUlJSUlJSUlJSUqKiUqKiUqKiUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!SgRmB49jMkpEGwajZUGOeFs1vKGra6qC9bvGoQ_-R5gmjutWm1SkLioAbZS2KOX3gA$" target="_blank" rel="noreferrer">9420
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<p class="MsoNormal">La Jolla, CA 92037<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal">***************************<u></u><u></u></p>
</div>
<div>
<p class="MsoNormal"><u></u> <u></u></p>
</div>
</div>
<p class="MsoNormal"><br>
<br>
<u></u><u></u></p>
</blockquote>
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<div>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<pre>_______________________________________________<u></u><u></u></pre>
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