<html><head><meta http-equiv="Content-Type" content="text/html; charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class="">Hi,</div><div class=""><br class=""></div>In the old days with film cameras, when the screen went up, the indicated magnification changed to reflect the magnification at the film camera plane. Any digital camera mounted below that plane would thus get a post-magnification, typically 1.4x for Philips/FEI/TFS instruments. Without film cameras (Glacios, Arctica, Krios) this behaviours has at some point been removed from the software. As stated by others, magnification is the worst thing to use as a parameter, we should just stick to calibrated physical pixel size of the detector. <div class=""><br class=""></div><div class="">Wim Hagen</div><div class="">EMBL Heidelberg<br class=""><div><br class=""><blockquote type="cite" class=""><div class="">On 24. Jun 2020, at 15:58, Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" class="">dagmorga@indiana.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div style="margin-top: 0px; margin-bottom: 0px; caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class="">Hi,</div><div style="margin-top: 0px; margin-bottom: 0px; caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class=""><br class=""></div><div style="margin-top: 0px; margin-bottom: 0px; caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class=""> Several people have reported what Zongli said about the mag changing when the screen is inserted. This does _not_ happen with our Artica and I think that it does not happen with most/all Glacios/Artica/Krios TEM's. Either ThermoFisher has deliberately turned off this behavior, or it is another bug in recent release of the software.<br class=""></div><div style="margin-top: 0px; margin-bottom: 0px; caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class=""><br class=""></div><div id="Signature" style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class=""><div name="divtagdefaultwrapper" style="font-family: Calibri, Arial, Helvetica, sans-serif; margin: 0px;" class=""><div name="divtagdefaultwrapper" style="font-family: Calibri, Arial, Helvetica, sans-serif; margin: 0px;" class="">-- </div><div name="divtagdefaultwrapper" style="font-family: Calibri, Arial, Helvetica, sans-serif; margin: 0px;" class=""> politics is more difficult than physics.</div><div name="divtagdefaultwrapper" style="font-family: Calibri, Arial, Helvetica, sans-serif; margin: 0px;" class=""> A. Einstein</div><div name="divtagdefaultwrapper" style="font-family: Calibri, Arial, Helvetica, sans-serif; margin: 0px;" class=""><br class=""><div name="divtagdefaultwrapper" style="font-family: Calibri, Arial, Helvetica, sans-serif; margin: 0px;" class=""> David Gene Morgan<br class=""> Electron Microscopy Center<br class=""> 047E Simon Hall<br class=""> IU Bloomington<br class=""> 812 856 1457 (office)<br class=""> 812 856 3221 (3200)<br class=""> <span class="Apple-converted-space"> </span><a href="https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!SjlQ155eJJt7G-lOVflOWQRvfeqzYhZqcJOIHQMJ1pyb1REIo0KAIgpLgeJ2RxUStg$" class="">http://iubemcenter.indiana.edu</a></div></div></div></div><div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none; color: rgb(33, 33, 33);" class=""><hr tabindex="-1" style="display: inline-block; width: 1005.46875px;" class=""><div id="divRplyFwdMsg" dir="ltr" class=""><font face="Calibri, sans-serif" style="font-size: 11pt;" class=""><b class="">From:</b><span class="Apple-converted-space"> </span>Michael Elbaum <<a href="mailto:michael.elbaum@weizmann.ac.il" class="">michael.elbaum@weizmann.ac.il</a>><br class=""><b class="">Sent:</b><span class="Apple-converted-space"> </span>Wednesday, June 24, 2020 3:20 AM<br class=""><b class="">To:</b><span class="Apple-converted-space"> </span>Zongli Li; Morgan, David Gene<br class=""><b class="">Cc:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><br class=""><b class="">Subject:</b><span class="Apple-converted-space"> </span>[External] Re: [3dem] magnification question</font><div class=""> </div></div><div class=""><div style="font-family: Arial, Helvetica, sans-serif; font-size: 12px; background-color: rgb(255, 236, 229); color: rgb(130, 39, 13); border-left-width: 0.25rem; border-left-style: solid; border-left-color: rgb(223, 54, 3); padding: 0.5rem; text-align: left; line-height: 1.25;" class="">This message was sent from a non-IU address. Please exercise caution when clicking links or opening attachments from external sources.</div><br class=""><div id="divtagdefaultwrapper" dir="ltr" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;" class=""><div style="margin-top: 0px; margin-bottom: 0px;" class="">Just a heads-up then... I was shocked to see that the magnification written into a file name by the Talos software depends on the size of the monitor attached. Maybe this is STEM-specific? It's very misleading. Altogether magnification is a slippery concept though. The pixel sampling is much less ambiguous: what length in the specimen does a pixel in the recorded file represent? Multiply by the number of pixels for the field of view. It's the same image whether you view on your phone or projected onto the wall.</div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><br class=""></div><div style="margin-top: 0px; margin-bottom: 0px;" class="">Michael<br class=""></div></div><hr tabindex="-1" style="display: inline-block; width: 1005.46875px;" class=""><div id="divRplyFwdMsg" dir="ltr" class=""><font face="Calibri, sans-serif" style="font-size: 11pt;" class=""><b class="">From:</b><span class="Apple-converted-space"> </span>3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" class="">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Zongli Li <<a href="mailto:zongli.li@gmail.com" class="">zongli.li@gmail.com</a>><br class=""><b class="">Sent:</b><span class="Apple-converted-space"> </span>Tuesday, June 23, 2020 11:38:26 PM<br class=""><b class="">To:</b><span class="Apple-converted-space"> </span>Morgan, David Gene<br class=""><b class="">Cc:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><br class=""><b class="">Subject:</b><span class="Apple-converted-space"> </span>Re: [3dem] magnification question</font><div class=""> </div></div><div class=""><div dir="ltr" class="">Hi David Gene,<div class=""><br class=""></div><div class="">The magnifications reported by a Talos Arctica user interface should be " mag on the viewing screen" if the viewing screen is inserted, and will change to " mag on the CCD" when viewing screen is extracted. But as Sacha pointed out, what matters is really the calibrated mag on the whatever camera you have used for calibration and data collection, and this mag will not show up in the user interface, but stored in the magnification table in Gatan GMS if you are using Gatan CCD/K2/K3 camera.</div><div class=""><br class=""></div><div class="">Best,</div><div class=""><br class=""></div><div class="">Zongli</div></div><br class=""><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Tue, Jun 23, 2020 at 1:50 PM Morgan, David Gene <<a href="mailto:dagmorga@indiana.edu" class="">dagmorga@indiana.edu</a>> wrote:<br class=""></div><blockquote class="gmail_quote" style="margin: 0px 0px 0px 0.8ex; border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204); padding-left: 1ex;"><div dir="ltr" style="font-size: 12pt; background-color: rgb(255, 255, 255); font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><div style="margin-top: 0px; margin-bottom: 0px;" class="">Hi,</div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><br class=""></div><div style="margin-top: 0px; margin-bottom: 0px;" class=""> Does anyone happen to know whether the magnifications reported by a Talos Artica are supposed to be "mag on the viewing screen" or "mag at the film plane"? We clearly have a serious issue with the calibrated magnifications on our microscope, but I just recalibrated it and got more or less the same lousy magnifications. I'm trying to understand our issues, and knowing what the microscope thinks it is reporting would be helpful. Thanks in advance.<br class=""></div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><br class=""></div><div id="gmail-m_5882692347410308242Signature" class=""><div name="divtagdefaultwrapper" class=""><div name="divtagdefaultwrapper" class="">-- </div><div name="divtagdefaultwrapper" class=""> politics is more difficult than physics.</div><div name="divtagdefaultwrapper" class=""> A. Einstein</div><div name="divtagdefaultwrapper" class=""><br class=""><div name="divtagdefaultwrapper" class=""> David Gene Morgan<br class=""> Electron Microscopy Center<br class=""> 047E Simon Hall<br class=""> IU Bloomington<br class=""> 812 856 1457 (office)<br class=""> 812 856 3221 (3200)<br class=""> <span class="Apple-converted-space"> </span><a href="https://urldefense.com/v3/__http://iubemcenter.indiana.edu__;!!Mih3wA!Q1NuuXpLaELUTGJgvoiPm1e0cHpDssbesoFay-9J7tLFPfHrCncsLjz01d4djDLt9Q$" target="_blank" class="">http://iubemcenter.indiana.edu</a></div></div></div></div></div>_______________________________________________<br class="">3dem mailing list<br class=""><a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" class="">3dem@ncmir.ucsd.edu</a><br class=""><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" rel="noreferrer" target="_blank" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br class=""></blockquote></div></div></div></div><span style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none; float: none; display: inline !important;" class="">_______________________________________________</span><br style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class=""><span style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none; float: none; display: inline !important;" class="">3dem mailing list</span><br style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class=""><span style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none; float: none; display: inline !important;" class=""><a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a></span><br style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class=""><span style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none; float: none; display: inline !important;" class=""><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a></span><br style="caret-color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration: none;" class=""></div></blockquote></div><br class=""></div></body></html>