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<p class="MsoNormal">Hello 3DEM Community.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We are exploring Graphenea grids (UltrAuFoils with graphene coating) and exposure to hydrogen plasmas. For specimens that were working fine with amorphous carbon films (lacey with carbon over the holes), we seem to get much less sample
on Graphenea grids after normal glow discharge (PelcoEasiglow – 45sec, 15mAmp, 0.39mBarr). We explored working with these same grids using 100% Hydrogen plasma (30 sccm, 100% power, 4e-4 Torr generated with Fischione 1070) for 10, 20 and 40 seconds. Unfortunately
with our specimen, we don’t see an increase in protein adsorption as previously reported (<a href="https://urldefense.com/v3/__https://pubmed.ncbi.nlm.nih.gov/24747813/__;!!Mih3wA!W2ZEvZsHaXOh6L3SLsRIkcAObX7QU78SQu2aDuyHKfGaqJYmCxM1Ug1O9XzNql1J8w$">https://pubmed.ncbi.nlm.nih.gov/24747813/</a>). We have the grids held in a Pelco Grid clamping block for plasma
treatment. Can anyone provide tips/tricks? Any other pretreatments needed? We are open to all suggestions as the cost of these grids is a concern.
<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks in advance,<o:p></o:p></p>
<p class="MsoNormal"><span style="color:#1F497D">Tommi A. White, Ph.D.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Director, Electron Microscopy Core<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Assistant Research Professor, Biochemistry<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">University of Missouri<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Mail: W117 Veterinary Medicine Bldg<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"> 1520 E Rollins St., Columbia, MO 65211<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">EMC: 573-882-8304<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Direct: 573-884-7338<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Email: </span><a href="mailto:whiteto@missouri.edu"><span style="color:#0563C1">whiteto@missouri.edu</span></a><span style="color:#1F497D"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Web: </span><a href="https://urldefense.com/v3/__http://emc.missouri.edu/__;!!Mih3wA!W2ZEvZsHaXOh6L3SLsRIkcAObX7QU78SQu2aDuyHKfGaqJYmCxM1Ug1O9XzVdiK9sw$"><span style="color:#0563C1">http://emc.missouri.edu</span></a><span style="color:#1F497D"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">Tweets:@MizzouEMC<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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