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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link="#0563C1" vlink="#954F72"><div class=WordSection1><p class=MsoNormal><span style='mso-fareast-language:ZH-CN'>Dear all,<o:p></o:p></span></p><p class=MsoNormal><span style='mso-fareast-language:ZH-CN'>I would like to post a microED commissioning call for proposals at eBIC/Diamond Light Source. Please see below for details. <o:p></o:p></span></p><p class=MsoNormal><span style='mso-fareast-language:ZH-CN'>Best,<o:p></o:p></span></p><p class=MsoNormal><span style='mso-fareast-language:ZH-CN'>Peijun<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal style='margin-bottom:.5pt;background:white'><b><span lang=EN-GB style='font-size:8.0pt;font-family:"Lucida Calligraphy";color:black;background:white;mso-fareast-language:EN-GB'>Peijun Zhang</span></b><b><span lang=EN-GB style='font-size:8.0pt;font-family:"Lucida Calligraphy";color:black;background:white;mso-fareast-language:EN-GB'><o:p></o:p></span></b></p><p class=MsoNormal style='margin-bottom:.5pt'><span style='font-size:8.0pt;mso-fareast-language:ZH-CN'>Director</span><span lang=EN-GB style='font-size:8.0pt;mso-fareast-language:ZH-CN'><o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:.5pt'><span style='font-size:8.0pt;mso-fareast-language:ZH-CN'>Electron Bio-Imaging Centre (eBIC)</span><span lang=EN-GB style='font-size:8.0pt;mso-fareast-language:ZH-CN'><o:p></o:p></span></p><p class=MsoNormal style='margin-bottom:.5pt'><span style='font-size:8.0pt;mso-fareast-language:ZH-CN'>Diamond Light Source</span><span lang=EN-GB style='font-size:8.0pt;mso-fareast-language:ZH-CN'><o:p></o:p></span></p><p class=MsoNormal><span lang=EN-GB style='font-size:8.0pt;color:#3366FF;mso-fareast-language:ZH-CN'><a href="https://urldefense.com/v3/__https://www.diamond.ac.uk/Instruments/Biological-Cryo-Imaging/eBIC.html__;!!Mih3wA!UjKUMoimKYtiWLedYSQNKwyyklOzDBnNbdbn0UyiLyT6Ruc9KhCYZQY9dAlU848OGg$"><span style='color:#3366FF'>https://www.diamond.ac.uk/Instruments/Biological-Cryo-Imaging/eBIC.html</span></a><o:p></o:p></span></p><p class=MsoNormal><span style='mso-fareast-language:ZH-CN'><o:p> </o:p></span></p><p class=MsoNormal><span style='mso-fareast-language:ZH-CN'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b><span style='mso-fareast-language:ZH-CN'>From:</span></b><span style='mso-fareast-language:ZH-CN'> Diamond User Office <USEROFFICE@diamond.ac.uk> <br><b>Sent:</b> Tuesday, June 16, 2020 2:55 PM<br><b>Subject:</b> MicroED commissioning: call for proposals<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><p style='margin-bottom:10.0pt;line-height:115%'><span lang=EN-GB style='color:black'>Dear eBIC users,</span><span lang=EN-GB style='font-family:"Times New Roman",serif;color:black'><o:p></o:p></span></p><p style='margin-bottom:10.0pt;text-align:justify;line-height:115%'><span lang=EN-GB style='color:black'>We are pleased to announce that a commissioning call for Micro-Electron Diffraction (MicroED) proposals, at eBIC, is now open. <b>The deadline for proposal submission is Friday 31<sup>st</sup> July 2020 17:00hrs BST.</b> This call will provide access to MicroED for a limited number of selected user proposals (Academic users only). The microED sessions will be remote only, no users are expected on site. Initially <b>only on-grid MicroED data collection with a Ceta-D detector </b></span><b><span style='color:black'>on a Talos Arctica (200 keV) will be available</span></b><b><span lang=EN-GB style='color:black'>.</span></b><span lang=EN-GB style='font-family:"Times New Roman",serif;color:black'><o:p></o:p></span></p><p style='margin-bottom:10.0pt;text-align:justify;line-height:115%'><span lang=EN-GB style='color:black'>Proposals will be independently evaluated with respect to scientific merit and sample suitability, with in-house assessment of technical feasibility. Applications should include evidence that the proposed samples are suitable for MicroED experiments. All grids must be pre-screened, demonstrating that they have the appropriate ice thickness, that the majority of the EM grid support film remains intact, and that there is a reasonable crystal distribution. The best way to show this is to include TEM grid atlas images and images of the crystals at higher magnifications in the proposal.</span><span lang=EN-GB style='font-family:"Times New Roman",serif;color:black'><o:p></o:p></span></p><p style='margin-bottom:10.0pt;text-align:justify;line-height:115%'><span lang=EN-GB style='color:black'>For MicroED commissioning proposals that require CryoFIB time to achieve the desired sample thickness, please indicate this in the application. A separate proposal for CryoFIB rapid access should also be submitted. More information about CryoFIB rapid access can be found <a href="https://urldefense.com/v3/__https://www.diamond.ac.uk/dam/jcr:0d471fe4-a77a-44f4-8a56-6d35e1dc0ad2/diamond_ebic_cryofib_information.pdf__;!!Mih3wA!UjKUMoimKYtiWLedYSQNKwyyklOzDBnNbdbn0UyiLyT6Ruc9KhCYZQY9dAkvjHl6Cg$" title="https://www.diamond.ac.uk/dam/jcr:0d471fe4-a77a-44f4-8a56-6d35e1dc0ad2/diamond_ebic_cryofib_information.pdf">here</a>.</span><span lang=EN-GB style='font-family:"Times New Roman",serif;color:black'><o:p></o:p></span></p><p style='margin-bottom:10.0pt;text-align:justify;line-height:115%'><span lang=EN-GB style='color:black'>If your proposal is successful, sessions will be allocated from October 2020. Sessions will run from 9:00 am to 17:00 pm, with a maximum of 4 grids per day. Please indicate the number of days you would like to request in the proposal (we will allocate between 1-2 days).</span><span lang=EN-GB style='font-family:"Times New Roman",serif;color:black'><o:p></o:p></span></p><p style='margin-bottom:10.0pt;text-align:justify;line-height:115%'><span lang=EN-GB style='color:black'>All proposals need to be submitted through the User Administrator System (<a href="https://urldefense.com/v3/__https://auth.diamond.ac.uk/cas/login?service=https*3A*2F*2Fuas.diamond.ac.uk*2Fuas*2Flogin*2Fcas__;JSUlJSUl!!Mih3wA!UjKUMoimKYtiWLedYSQNKwyyklOzDBnNbdbn0UyiLyT6Ruc9KhCYZQY9dAlKI8QVkA$" title="https://auth.diamond.ac.uk/cas/login?service=https%3A%2F%2Fuas.diamond.ac.uk%2Fuas%2Flogin%2Fcas">UAS</a>). Once logged into the UAS you should choose the “commissioning” access route for the Talos Arctica instrument. You will be notified of the outcome of your application by mid-August.</span><span lang=EN-GB style='font-family:"Times New Roman",serif;color:black'><o:p></o:p></span></p><p style='margin-bottom:10.0pt;text-align:justify;line-height:115%'><span lang=EN-GB style='color:black'>For technical inquiries about proposed experiments please contact </span><span style='font-family:"Times New Roman",serif;color:black'><a href="about:blank"><span lang=EN-GB style='font-family:"Calibri",sans-serif'>Yun.Song@diamond.ac.uk</span></a></span><span lang=EN-GB style='color:black'>. </span><span lang=EN-GB style='font-family:"Times New Roman",serif;color:black'><o:p></o:p></span></p><p style='margin-bottom:10.0pt;line-height:115%'><span lang=EN-GB style='color:black'>Please see below the bioRxiv preprint for information about microED workflow at eBIC: </span><span style='font-family:"Times New Roman",serif;color:black'><a href="about:blank"><span lang=EN-GB style='font-family:"Calibri",sans-serif'>https://biorxiv.org/cgi/content/short/2020.04.30.061895v1</span></a></span><span lang=EN-GB style='font-family:"Times New Roman",serif;color:black'><o:p></o:p></span></p><p class=MsoNormal><span lang=EN-GB style='font-size:12.0pt;color:black'><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-GB style='font-size:12.0pt;color:black'>Kind regards,<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-GB style='font-size:12.0pt;color:black'><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-GB style='font-size:12.0pt;color:black'>Diamond User Office on behalf of<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-GB style='font-size:12.0pt;color:black'>Yun Song<o:p></o:p></span></p><p class=MsoNormal><span lang=EN-GB style='font-size:12.0pt;color:black'><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-GB style='font-size:12.0pt;color:black'><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-GB style='mso-fareast-language:EN-GB'>E-mail: <a href="mailto:useroffice@diamond.ac.uk"><span style='color:blue'>useroffice@diamond.ac.uk</span></a><o:p></o:p></span></p><p class=MsoNormal><span lang=EN-GB style='mso-fareast-language:EN-GB'>web: <a href="https://urldefense.com/v3/__http://www.diamond.ac.uk/__;!!Mih3wA!UjKUMoimKYtiWLedYSQNKwyyklOzDBnNbdbn0UyiLyT6Ruc9KhCYZQY9dAk7hLRUCg$"><span style='color:blue'>www.diamond.ac.uk</span></a><o:p></o:p></span></p><p class=MsoNormal><b><i><span lang=EN-GB style='font-size:10.0pt;mso-fareast-language:EN-GB'><o:p> </o:p></span></i></b></p><p class=MsoNormal><b><span lang=EN-GB style='font-size:10.0pt;mso-fareast-language:EN-GB'>Diamond User Office is open Mon – Fri 08:30 – 16:30 </span></b><span lang=EN-GB style='font-size:10.0pt;mso-fareast-language:EN-GB'>(closed Sat - Sun and on UK public holidays)</span><i><span lang=EN-GB style='mso-fareast-language:EN-GB'><o:p></o:p></span></i></p><p class=MsoNormal><b><span lang=EN-GB style='font-size:10.0pt;mso-fareast-language:EN-GB'>We will<i> </i>aim to respond<i> to your enquiry within 24 </i>hours<i>. If you contact us outside of </i>office hours<i>, we </i>aim to respond<i> on the next </i>working<i> day.</i></span></b><b><i><span lang=EN-GB style='mso-fareast-language:EN-GB'><o:p></o:p></span></i></b></p><p class=MsoNormal><span lang=EN-GB style='mso-fareast-language:EN-GB'><o:p> </o:p></span></p><p class=MsoNormal><span lang=EN-GB style='mso-fareast-language:EN-GB'><img border=0 width=309 height=43 style='width:3.2187in;height:.4479in' id="Picture_x0020_1" src="cid:image001.png@01D643EB.E42D5500"></span><span lang=EN-GB style='mso-fareast-language:EN-GB'><o:p></o:p></span></p><p class=MsoNormal><span lang=EN-GB><o:p> </o:p></span></p></div></body></html>