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<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Thank you Pam, Matthijn and others for your answers!<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">I was not very clear in my question and would like to granulate that we are planning to install a Thermofisher Glacios microscope and are currently designing a lab.
We are searching for an optimum way to arrange the refill of the LN2 tanks that supply the microscopes. Would people like to share their setup with us? In our building it might not be possible to move the nitrogen tanks and we might need to build a refill
station near/next to it.<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Thank you in advance for all your comments and suggestions!<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;mso-fareast-language:EN-US">a<o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="color:black">From: </span></b><span style="color:black">Pamela J Focia <focia@northwestern.edu><br>
<b>Date: </b>Thursday 11 June 2020 at 17:28<br>
<b>To: </b>Alexey Rak <Alexey.Rak@sanofi.com><br>
<b>Cc: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[EXTERNAL] Re: N2 refilling system<o:p></o:p></span></p>
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<p><b><span style="color:red">EXTERNAL : </span></b><span style="color:black">Real sender is focia@northwestern.edu
</span><o:p></o:p></p>
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<p>Hi Alexey,<o:p></o:p></p>
<p><o:p> </o:p></p>
<p>At Northwestern University in the Structural Biology Facility we use the Norhoff LN2 dispensing system and can run continuous data collection for days at a time. It is fantastic!<o:p></o:p></p>
<p><o:p> </o:p></p>
<p>We have two, one for the sample holder and one for ACD. Each has a sensor placed inside alongside a small plastic funnel, and when needed the LN2 drips out into the funnels until full.<o:p></o:p></p>
<p><o:p> </o:p></p>
<p><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__www.norhof.com_product_900-2Dautomatic-2Dadvance-2Dln2-2Dmicrodosing-2Dpump_&d=DwMFAw&c=Dbf9zoswcQ-CRvvI7VX5j3HvibIuT3ZiarcKl5qtMPo&r=y0zK4lu9MOBbGiAn8bD_nZlA_GPScVN5ad5lZv0_qms&m=6crFnOx5eZg0uuQ0OZw83HPGMCO5tcp_MzZBIu1XV5w&s=9qTJyGiW-zo2PrFyPTucrAyiWgEMPQynYq4hv2buqaI&e=">https://www.norhof.com/product/900-automatic-advance-ln2-microdosing-pump/</a><o:p></o:p></p>
<p>​<o:p></o:p></p>
<p>-pam<o:p></o:p></p>
<p><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:10.0pt">---------------------------------<br>
-------------------<br>
<br>
Pamela J. Focia, Ph.D.<br>
Research Associate Professor<br>
Biochemistry and Molecular Genetics​<br>
<br>
Structural Biology Facility Manager<br>
Robert H. Lurie Comprehensive Cancer Center</span><span style="font-size:11.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt">Feinberg School of Medicine<br>
Northwestern University<br>
303 E. Chicago Ave, Chicago 60611<br>
c (312)286-3274<br>
o (312)503-0848 </span><span style="font-size:11.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.0pt"><a href="mailto:focia@northwestern.edu">focia@northwestern.edu</a></span><span style="font-size:11.0pt"><o:p></o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;color:black">From:</span></b><span style="font-size:11.0pt;color:black"> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Rak, Alexey /FR <Alexey.Rak@sanofi.com><br>
<b>Sent:</b> Thursday, June 11, 2020 1:09 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] N2 refilling system</span><span style="font-size:11.0pt;color:#212121">
<o:p></o:p></span></p>
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<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;color:#212121">Dear 3dem Community,</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;color:#212121">We are looking for an ideal solution to refill N2 tank used for CryoEMscope and would appreciate any suggestions, examples, links that you may share with us.</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;color:#212121">Thank you in advance.</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:11.0pt;color:#212121">alexey</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:14.0pt;font-family:"Brush Script MT";color:#262087"> </span></b><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal"><b><span lang="EN-US" style="font-size:14.0pt;font-family:"Brush Script MT";color:#262087"> Alexey RAK, PhD</span></b><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:21.3pt;background:white"><span lang="IT" style="font-size:9.0pt;font-family:"CG Times";color:#1F497D">Head of Bio Structure and Biophysics</span><span style="color:#212121"><o:p></o:p></span></p>
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<p class="MsoNormal" style="margin-left:21.3pt;background:white"><span lang="IT" style="font-size:9.0pt;font-family:"CG Times";color:#1F497D">Sanofi R&D </span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:21.3pt;background:white"><span lang="FR" style="font-size:9.0pt;font-family:"CG Times";color:#1F497D">13, Quai Jules Guesde - BP 14 Office: +33 (0) 1 58 93 86 93</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:21.3pt;background:white"><span lang="FR" style="font-size:9.0pt;font-family:"CG Times";color:#1F497D">94403 Vitry sur Seine Cedex Cell: +33 (0) 6 82 61 51 34</span><span style="color:#212121"><o:p></o:p></span></p>
<p class="MsoNormal" style="margin-left:21.3pt;background:white"><span lang="FR" style="font-size:9.0pt;font-family:"CG Times";color:#1F497D">FRANCE <a href="mailto:Alexey.Rak@sanofi.com"><span style="color:#0563C1">Alexey.Rak@sanofi.com</span></a></span><span style="color:#212121"><o:p></o:p></span></p>
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