<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=Windows-1252">
<style type="text/css" style="display:none;"> P {margin-top:0;margin-bottom:0;} </style>
</head>
<body dir="ltr">
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
Hi - we use an ACE600 for carbon-coating TEM grids and iridium coating SEM samples. In general I'm happy with the results, especially with the metal coating. The carbon coating uses carbon strings, so there might be some variations between coats, especially
 by different operators who may set the springs slightly differently, but generally in controllable range.  We have ten people or so using it. I guess if you are the only one who uses it, it can be much more problem free. Greg</div>
<div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<br>
</div>
<div id="Signature">
<div></div>
<div></div>
<div id="divtagdefaultwrapper" dir="ltr" style="font-size:12pt; color:#000000; font-family:Calibri,Helvetica,sans-serif">
<p style="margin-top:0px; margin-bottom:0px; margin-top:0; margin-bottom:0"></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt">Dr. Gang (Greg) Ning</span></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt">Director</span></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt">Microscopy Facility</span></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt">Huck Institutes of the Life Sciences</span></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt">Penn State University</span></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt">MSC N-048</span></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt">University Park, PA 16802</span></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt">814-863-0994</span></p>
<p style="margin-top:0px; margin-bottom:0px; font-family:Calibri,Helvetica,sans-serif,EmojiFont,"Apple Color Emoji","Segoe UI Emoji",NotoColorEmoji,"Segoe UI Symbol","Android Emoji",EmojiSymbols; font-size:16px">
<span style="font-size:11pt"></span><a href="https://urldefense.com/v3/__https://www.huck.psu.edu/core-facilities/microscopy-facility__;!!Mih3wA!WWWCmoeA-Gq2NLhiPiPYJaVk5765gze2Y_NgWVheATWp8r9huEcMh_jGt8xXkimE-w$" class="OWAAutoLink"><span style="font-size:11pt">https://www.huck.psu.edu/core-facilities/microscopy-facility</span></a><br>
</p>
<div><br>
</div>
<br>
<p style="margin-top:0px; margin-bottom:0px"></p>
</div>
</div>
</div>
<div id="appendonsend"></div>
<hr style="display:inline-block;width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Dienemann, Christian <christian.dienemann@mpibpc.mpg.de><br>
<b>Sent:</b> Thursday, June 4, 2020 4:19 AM<br>
<b>To:</b> Morgan, David Gene <dagmorga@indiana.edu>; 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> Re: [3dem] carbon coating devices</font>
<div> </div>
</div>
<style>
<!--
@font-face
        {font-family:"Cambria Math"}
@font-face
        {font-family:Calibri}
p.x_MsoNormal, li.x_MsoNormal, div.x_MsoNormal
        {margin:0in;
        margin-bottom:.0001pt;
        font-size:12.0pt;
        font-family:"Times New Roman",serif}
a:link, span.x_MsoHyperlink
        {color:blue;
        text-decoration:underline}
a:visited, span.x_MsoHyperlinkFollowed
        {color:purple;
        text-decoration:underline}
p
        {margin:0in;
        margin-bottom:.0001pt;
        font-size:12.0pt;
        font-family:"Times New Roman",serif}
p.x_msonormal0, li.x_msonormal0, div.x_msonormal0
        {margin:0in;
        margin-bottom:.0001pt;
        font-size:12.0pt;
        font-family:"Times New Roman",serif}
span.x_EmailStyle19
        {font-family:"Calibri",sans-serif;
        color:#1F497D}
.x_MsoChpDefault
        {font-size:10.0pt}
@page WordSection1
        {margin:1.0in 1.0in 1.0in 1.0in}
div.x_WordSection1
        {}
-->
</style>
<div lang="EN-US" link="blue" vlink="purple" style="background-color:white">
<div class="x_WordSection1">
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D">Hi David,</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D"> </span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D">We use the ACE600 (carbon thread configuration) routinely for making thin carbon support foils. It is super simple, works like a charm and gives very reproducible
 results (thickness, floatability, homogeneity etc.). Foils with 2-2.5 nm thickness are no problem.</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D"> </span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D">As a facility manager, I also like to point out that I basically don’t need to spend much time maintaining or trouble shooting the instrument (1 day per year
 maybe).</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D"> </span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D">Best,</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D">Chris</span></p>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D"> </span></p>
<div>
<p class="x_MsoNormal"><span style="font-size:9.0pt; font-family:"Calibri",sans-serif; color:#595959">Dr. Christian Dienemann
<br>
Max-Planck-Institute for Biophysical Chemistry <br>
Department of Molecular Biology / Cramer lab<br>
Am Fassberg 11 <br>
37077 Göttingen, Germany <br>
+49-551-201-2819</span></p>
</div>
<p class="x_MsoNormal"><span style="font-size:11.0pt; font-family:"Calibri",sans-serif; color:#1F497D"> </span></p>
<div>
<div style="border:none; border-top:solid #E1E1E1 1.0pt; padding:3.0pt 0in 0in 0in">
<p class="x_MsoNormal"><b><span style="font-size:11.0pt; font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt; font-family:"Calibri",sans-serif"> 3dem <3dem-bounces@ncmir.ucsd.edu>
<b>On Behalf Of </b>Morgan, David Gene<br>
<b>Sent:</b> Wednesday, June 3, 2020 11:23 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] carbon coating devices</span></p>
</div>
</div>
<p class="x_MsoNormal"> </p>
<p><span style="font-family:"Calibri",sans-serif; color:black">Hi,</span></p>
<p><span style="font-family:"Calibri",sans-serif; color:black"> </span></p>
<p><span style="font-family:"Calibri",sans-serif; color:black">    Does anyone have experience with the Leica ACE600 carbon coater/metal sputter coater?  I am very wary of buying a dual purpose device like this as our only way to make carbon films for TEM grids,
 but I may be being overly cautious.  If anyone has thoughts or experiences, please let me know.</span></p>
<p><span style="font-family:"Calibri",sans-serif; color:black"> </span></p>
<div id="x_Signature">
<div name="x_divtagdefaultwrapper">
<div name="x_divtagdefaultwrapper">
<p class="x_MsoNormal"><span style="font-family:"Calibri",sans-serif; color:black">-- </span></p>
</div>
<div name="x_divtagdefaultwrapper">
<p class="x_MsoNormal"><span style="font-family:"Calibri",sans-serif; color:black">    politics is more difficult than physics.</span></p>
</div>
<div name="x_divtagdefaultwrapper">
<p class="x_MsoNormal"><span style="font-family:"Calibri",sans-serif; color:black">                                             A. Einstein</span></p>
</div>
<div name="x_divtagdefaultwrapper">
<p class="x_MsoNormal"><span style="font-family:"Calibri",sans-serif; color:black"> </span></p>
<div name="x_divtagdefaultwrapper">
<p class="x_MsoNormal"><span style="font-family:"Calibri",sans-serif; color:black">            David Gene Morgan<br>
        Electron Microscopy Center<br>
             047E Simon Hall<br>
             IU Bloomington<br>
          812 856 1457 (office)<br>
          812 856 3221 (3200)<br>
      </span><a href="https://urldefense.com/v3/__https://nam01.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__http*3A*2Fiubemcenter.indiana.edu__*3B!!Mih3wA!UQ9Qrot0OBWFZRBfeQ73s2RYuHMDMGc9lAM5US2gCul9XQ4Mh_K-LuH5A41cqjuECg*24&data=02*7C01*7Cgxn7*40psu.edu*7Cc184fd4a8b3947244b9508d8086010ed*7C7cf48d453ddb4389a9c1c115526eb52e*7C0*7C0*7C637268555995554930&sdata=vCJOQsyFFBw5Hv4s3qROo*2FkZuKpHTYFPWgEyeqUxv8g*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!WWWCmoeA-Gq2NLhiPiPYJaVk5765gze2Y_NgWVheATWp8r9huEcMh_jGt8z70gPasg$" originalsrc="https://urldefense.com/v3/__http:/iubemcenter.indiana.edu__;!!Mih3wA!UQ9Qrot0OBWFZRBfeQ73s2RYuHMDMGc9lAM5US2gCul9XQ4Mh_K-LuH5A41cqjuECg$" shash="Jd3hA9yPwkXDppaDGY0HQemX70t3x32C+Q+3ScrS/6itSJxOOXpE1B7cOXn1z57rMI52pEBgD+uvgRYo3FTAZ+SQtnV/Lovausgb5ypSqYZXLhoR5vNYP27XT6DQIx1S10xs8mZvD1G2v9rHrx2MeP+hdtY3kiEbOD/8fBNYjOI="><span style="font-family:"Calibri",sans-serif">http://iubemcenter.indiana.edu</span></a><span style="font-family:"Calibri",sans-serif; color:black"></span></p>
</div>
</div>
</div>
</div>
</div>
</div>
</body>
</html>