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<p><span style="font-size:12pt">Hello,</span></p>
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<p>I hope all is safe. If you would please advise on the following:</p>
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<p>I made auto-refinement for a structure u<span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:16px; background-color:rgb(255,255,255)">sing auto-sampling settings: I</span><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:16px; background-color:rgb(255,255,255)">nitial
angular sampling</span><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:16px; background-color:rgb(255,255,255)"> 3.</span><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:16px; background-color:rgb(255,255,255)">7,
local searching from auto-sampling 1.8. T</span>he output after convergence<span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:16px; background-color:rgb(255,255,255)"> was "Final resolution (without masking) is: 3.26154</span>)". When I
generate a mask using binarisation threshold from 0.001 to 0.005, where 0.005 gave warning, the FSC curve has a prominent peak in the FSC plot. I tried changing these parameters and tried the output from other refinement job with bigger box but all give this
peak.<br>
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<p>my questions<br>
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<p>1- What caused and how to get rid of this peak?<br>
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<p>2- If this peak problem could not be fixed, can I use proceed with such post-process.star file for ctf refinement and polishing?</p>
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<p>Thank you very much for your help and time. <br>
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<p>Last iteration before conversion: <br>
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<div> CurrentResolution= 3.93402 Angstroms, which requires orientationSampling of at least 2.04545 degrees for a particle of diameter 220 Angstroms</div>
<div> Oversampling= 0 NrHiddenVariableSamplingPoints= 1305</div>
<div> OrientationalSampling= 0.46875 NrOrientations= 145</div>
<div> TranslationalSampling= 0.4605 NrTranslations= 9</div>
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<div> Oversampling= 1 NrHiddenVariableSamplingPoints= 41760</div>
<div> OrientationalSampling= 0.234375 NrOrientations= 1160</div>
<div> TranslationalSampling= 0.23025 NrTranslations= 36<br>
<span style="font-size:12pt">E</span><span style="font-size:12pt">xpectation iteration 25</span><br>
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