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If you’re looking for a particularly challenging case, you may want to look at this dataset of assembling ribosomal subunits:
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<div class=""><a href="https://urldefense.com/v3/__https://www.ebi.ac.uk/pdbe/emdb/empiar/entry/10076/__;!!Mih3wA!V6DsOkbPkp5bM6XomxT1ySP3Ya2Vlzhi-14V_OVrfdNsjDvLhfscR78RG1nlJz4ECQ$" class="">https://www.ebi.ac.uk/pdbe/emdb/empiar/entry/10076/</a></div>
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<div class="">There are at least 14 distinct structures there, but I’m sure you can find more. </div>
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<div class="">Best,</div>
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<div class="">Dmitry </div>
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Dmitry Lyumkis</div>
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The Salk Institute for Biological Studies</div>
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E: <a href="mailto:dlyumkis@salk.edu" class="">dlyumkis@salk.edu</a></div>
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<div class="">On Apr 20, 2020, at 11:10 AM, Takanori Nakane <<a href="mailto:tnakane@mrc-lmb.cam.ac.uk" class="">tnakane@mrc-lmb.cam.ac.uk</a>> wrote:</div>
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<div class="">Hi,<br class="">
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How different should it be?<br class="">
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Different species:<br class="">
EMPIAR-10251: mixture of 20S proteasome and TMV<br class="">
EMPIAR 10289-10291: innexin hemichannel but contains dimeric full channels as well<br class="">
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Conformational difference:<br class="">
EMPIAR-10180: spliceosome with flexibility<br class="">
EMPIAR-10160: spliceosome, even more flexibility<br class="">
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And there are several ribosome datasets as well.<br class="">
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Best regards,<br class="">
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Takanori Nakane<br class="">
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On 2020/04/20 19:03, rupesh wrote:<br class="">
<blockquote type="cite" class="">Dear all,<br class="">
I have been working on 3D reconstructions from 2d projections obtained by Cryo Electron Microscopy, specially dealing with the case when there are multiple conformations of the particle in the projections obtained and hence multiple 3D structures to be generated.<br class="">
I have obtained good classifications results in case of projections obtained for different particles from different micrographs, but to test the classifier in its full capacity, I need a micrograph which has 2 or more types of picked particles, and hence 2
or more 3D structure lying in the set of projections that can be obtained.<br class="">
Any help or pointers to correct resources will be highly appreciated.<br class="">
Regards<br class="">
Rupesh<br class="">
4th Year UnderGrad<br class="">
Computer Science<br class="">
IIT Bombay<br class="">
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