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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><div><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Dear cryo-EM community<o:p></o:p></span></p><div><p class=MsoNormal><span style='font-family:"Arial",sans-serif'><o:p> </o:p></span></p></div><div><div><div><div><div><div><div><p class=MsoNormal><span style='font-family:"Arial",sans-serif;color:black'>The Molecular Foundry is offering free support (human and computing power) for emergency COVID-19 research via processing users’ cryo-electron tomography (cryo-ET) data for 3D reconstruction at a single-molecule level (no averaging from different molecule, at a low to intermediate 3D resolution). Our specialized support includes <b>low-contrast image enhancement</b>, <b>individual-particle</b> <b>3D reconstruction </b>and <b>missing-wedge correction</b> by in-house software that was developed for handling the floppy macromolecules with<b> structural heterogeneity </b>or <b>variety</b> including <b>viral infection of a cell</b>. Combined with our experience molecular structural modeling and molecular dynamics simulations, we may help illuminate the molecular mechanism. <o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif;color:black'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif;color:black'>All work will take place remotely. Molecular Foundry staff will process cryo-ET data from users remotely and discuss through video conferencing. All results will belong to users.</span><o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p style='margin:0in;margin-bottom:.0001pt'><span style='font-family:"Arial",sans-serif;color:black'>References</span><o:p></o:p></p><ul style='margin-top:0in' type=square><li style='color:#333333;margin-top:0in;margin-bottom:0in;margin-left:-.25in;margin-bottom:.0001pt;mso-list:l0 level1 lfo1;background:white'><em><span style='font-family:"Arial",sans-serif'><a href="https://www.ncbi.nlm.nih.gov/pubmed/30420636"><span style='color:#666699;font-style:normal'>An Algorithm for Enhancing the Image Contrast of Electron Tomography, </span></a></span></em><span style='font-family:"Arial",sans-serif'> H. Wu, et. al., <em><span style='font-family:"Arial",sans-serif;font-style:normal'>Scientific Reports.</span></em><i>,</i> (2018), 8(1):16711, PMID: 30420636<o:p></o:p></span></li><li style='margin-top:0in;margin-bottom:0in;margin-left:-.25in;margin-bottom:.0001pt;mso-list:l0 level1 lfo1;background:white'><em><span style='font-family:"Arial",sans-serif;color:#333333'><a href="https://www.ncbi.nlm.nih.gov/pubmed/30557627"><span style='color:#666699;font-style:normal'>Single-Molecule 3D Imaging of Human Plasma Intermediate-Density Lipoproteins Reveals a Polyhedral Structure, </span></a></span></em><span style='font-family:"Arial",sans-serif;color:#333333'>D. Lei, et. al<i>., </i><em><span style='font-family:"Arial",sans-serif;font-style:normal'>BBA Molecular and Cell Biology of Lipids </span></em>, (2019), 1864(3):260-270</span><span style='font-family:"Arial",sans-serif'><o:p></o:p></span></li><li style='margin-top:0in;margin-bottom:0in;margin-left:-.25in;margin-bottom:.0001pt;mso-list:l0 level1 lfo1'><span style='font-family:"Arial",sans-serif'><a href="http://www.nature.com/articles/s41467-018-03018-0"><span style='color:#1155CC'>3D Structural Dynamics of DNA Origami Mechanisms and Machines Using Individual-Particle Electron Tomography, </span></a><span style='color:black'>D. Lei, et. al., Nature Communications, (2018), 9:592, DOI: 10.1038/s41467-018-03018-0</span><o:p></o:p></span></li><li style='margin-top:0in;margin-bottom:0in;margin-left:-.25in;margin-bottom:.0001pt;mso-list:l0 level1 lfo1'><span style='font-family:"Arial",sans-serif'><a href="http://www.ncbi.nlm.nih.gov/pubmed/27143480"><span style='color:#1155CC'>Insights into the Tunnel Mechanism of Cholesteryl Ester Transfer using All-atom Molecular Dynamics Simulations</span></a><span style='color:black'>, D. Lei, et. al.,, Journal of Biological Chemistry, (2016), 291(27):14034-44, doi: 10.1074</span><o:p></o:p></span></li></ul><p class=MsoNormal><span style='font-family:"Arial",sans-serif'><o:p> </o:p></span></p></div></div></div></div></div></div></div></div><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Best regards<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Gary<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Gang (Gary) Ren, Staff Scientist, P.I.<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Lawrence Berkeley National Laboratory<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Molecular Foundry, Room: 2220<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>1 Cyclotron Road, MS 67R2206<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Berkeley CA 94720-8197<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Phone: (510) 495-2375; Fax: (510) 486-7268<o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'>Email: <a href="mailto:gren@lbl.gov"><span style='color:#0563C1'>gren@lbl.gov</span></a>; </span><span style='font-family:"Arial",sans-serif'><a href="https://rengroup.lbl.gov/"><span style='color:#0563C1'>https://rengroup.lbl.gov/</span></a><o:p></o:p></span></p><p class=MsoNormal><span style='font-family:"Arial",sans-serif'><o:p> </o:p></span></p><div><p class=MsoNormal><span style='font-family:"Arial",sans-serif'><o:p> </o:p></span></p></div></div></body></html>