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<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">Dear all,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">this is to announce that we are opening our cryo-EM facilities for Covid-19 projects at
the Centre for Integrative Biology, Illkirch/Strasbourg, France, which hosts the French and European Infrastructures for Integrated Structural Biology, FRISBI, Instruct-ERIC and iNext-Discovery.<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">This can include for example work on Covid-19 related enzyme targets, genome-free capsid,
membrane receptor complexes, antibody complexes etc. (i.e. non-infectious samples).<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">Cryo-EM samples (on grids) should be sent in with dewars as no on-site visits by users are
possible. We also offer freezing and screening of grids for purified samples.<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">To apply please see below.<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">With best regards,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">Bruno Klaholz<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif"><o:p> </o:p></span></font></p>
<p class="MsoNormal" style="background:white"><u><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">Cryo-EM facilities:</span></font></u><font color="#212121" face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif;color:#212121"><o:p></o:p></span></font></p>
<p class="MsoNormal" style="background:white"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">The Centre for Integrative Biology (CBI) at IGBMC, Illkirch/Strasbourg,
France, provides a cutting-edge scientific and technological environment in integrated structural biology to address the structure and function of biological systems, notably on gene expression, from the atomic, molecular to the tissue scales. The CBI
</span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif"><a href="http://www.igbmc.fr/grandesstructures/cbi/"><span lang="EN-US">http://www.igbmc.fr/grandesstructures/cbi/</span></a></span></font><font color="#212121" face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif;color:#212121">,
hosts the French and European Infrastructures for Integrated Structural Biology, FRISBI
</span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif"><a href="http://frisbi.eu/"><span lang="EN-US">http://frisbi.eu/</span></a></span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif">,
<font color="#212121"><span style="color:#212121">Instruct</span></font>-ERIC<font color="#212121"><span style="color:#212121">
</span></font></span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif"><a href="https://www.structuralbiology.eu/"><span lang="EN-US">https://www.structuralbiology.eu/</span></a></span></font><font color="#212121" face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif;color:#212121"> </span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif">and
iNext-Discovery </span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif"><a href="https://instruct-eric.eu/news/eu-inext-discovery-grant-provides-technologies-for-key-research-in-structural-biology/"><span lang="EN-US">https://instruct-eric.eu/news/eu-inext-discovery-grant-provides-technologies-for-key-research-in-structural-biology/</span></a></span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif">,
<font color="#212121"><span style="color:#212121">which comprises advanced electron microscopy facilities equipped with cutting-edge instrumentation, see
</span></font></span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif"><a href="http://frisbi.eu/centers/instruct-center-france-1-igbmc/cryo-electron-microscopy/">http://frisbi.eu/centers/instruct-center-france-1-igbmc/cryo-electron-microscopy/</a></span></font><font color="#212121" face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif;color:#212121">.
Nodes of FRISBI and Instruct-ERIC also provide access for sample production, characterisation and structural analysis, and for nanobody production.<o:p></o:p></span></font></p>
<p class="MsoNormal" style="background:white"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">For Covid-19 projects we specifically provide acces to our Titan Krios microscope, which
is equipped with Cs corrector, Falcon </span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif">3<font color="#212121"><span style="color:#212121"> camera, GIF energy filter, K3 camera and phase plate.
<o:p></o:p></span></font></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">For purified samples (not yet on cryo-EM grids) we offer freezing (Vitrobot system) and
screening of grids.<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">The non-infectious character of the samples needs to be confirmed by the director of the
applying institute.<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">Please include a short description of the project and the characterisation of the sample
(biophysically characterisation and images / class averages of cryo-EM tests if available). Applications will be handled confidentially as for any applications.<o:p></o:p></span></font></p>
<p class="MsoPlainText"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoPlainText"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">The EM facility has a suite of associated equipments for sample preparation and dedicated
computing resources for image processing and 3D reconstruction by single particle cryo-EM and cryo electron tomography (cryo-ET)</span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif"> and software developements
including on-the-fly data processing<font color="#212121"><span style="color:#212121">.<o:p></o:p></span></font></span></font></p>
<p class="MsoNormal" style="background:white"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal" style="background:white"><font size="2" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:11.0pt;font-family:"Times New Roman",serif;color:#212121">Project applications should be sent via
</span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif">the FRISBI portal
</span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif"><a href="https://frisbi.eu/submit-a-proposal/">https://frisbi.eu/submit-a-proposal/</a></span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif">
<span lang="EN-US">(contact <a href="mailto:pmarie@igbmc.fr">pmarie@igbmc.fr</a> ) or Instruct-ERIC (</span></span></font><font color="black" face="Times New Roman"><span style="font-family:"Times New Roman",serif;color:black">click <a href="http://email.mail.aria.services/c/eJw1jbEOgjAURb8GNgnY0sLQQRMYnExQI2zP1yKtBUlpE_17u5ic4eQkN1eK6sEh1QI-pOuNCeZefC_1tTkjniaAdVCPEU1_7OY8cGPosDYJzfWyeRfQM3RFOglZj5yqvAJWKqCVik6oLEnNOKOMkdSKyft1S8gh2beR_36nnMZMhZi8A3wpF22A29by1Am0EKQGq5Z4-ZxB2wzf8w9shDpc" target="_blank"><font color="#51a7f9"><span style="color:#51A7F9">here</span></font></a>) </span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif">or
via iNext-Discovery: </span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif"><a href="https://inext-discovery.eu/network/inext-d/home">https://inext-discovery.eu/network/inext-d/home</a> (specific Covid-19 call to come
out shortly)</span></font><font color="#212121" face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif;color:#212121">.
</span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif">Please contact
<a href="mailto:pmarie@igbmc.fr">pmarie@igbmc.fr</a> or </span></font><font face="Times New Roman"><span style="font-family:"Times New Roman",serif"><a href="mailto:klaholz@igbmc.fr"><span lang="EN-US">klaholz@igbmc.fr</span></a></span></font><font color="#212121" face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif;color:#212121">
if you have </span></font><font face="Times New Roman"><span lang="EN-US" style="font-family:"Times New Roman",serif">any
<font color="#212121"><span style="color:#212121">questions.<o:p></o:p></span></font></span></font></p>
<p class="MsoNormal" style="background:white"><font size="3" color="#212121" face="Times New Roman"><span lang="EN-US" style="font-size:12.0pt;font-family:"Times New Roman",serif;color:#212121"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span lang="EN-US" style="font-size:11.0pt"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">###########################################################################<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">Bruno P. Klaholz<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">Centre for Integrative Biology<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">Department of Integrated Structural Biology<br>
Institute of Genetics and of Molecular and Cellular Biology<br>
IGBMC - UMR 7104 - U 1258<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">1, rue Laurent Fries<br>
BP 10142 <br>
67404 ILLKIRCH CEDEX<br>
FRANCE<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt">Tel. from abroad:<b><font color="red"><span style="color:red;font-weight:bold">
</span></font></b>0033.369.48.52.78<br>
Tel. inside France: 03.69.48.52.78<br>
websites:<br>
<a href="http://igbmc.fr/Klaholz" target="_blank"><font color="#3a2eb5"><span style="color:#3A2EB5">http://igbmc.fr/Klaholz</span></font></a><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt"><a href="http://www.igbmc.fr/grandesstructures/cbi/" target="_blank"><font color="blue"><span style="color:blue">http://www.igbmc.fr/grandesstructures/cbi</span></font></a><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt"><a href="http://frisbi.eu/" target="_blank"><font color="blue"><span style="color:blue">http://frisbi.eu</span></font></a><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt"><a href="http://instruct-eric.eu"><font color="blue"><span style="color:blue">http://instruct-eric.eu</span></font></a></span></font><font color="black" face="Times New Roman"><span style="font-family:"Times New Roman",serif;color:black"><o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Calibri"><span style="font-size:11.0pt"><o:p> </o:p></span></font></p>
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