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We have two made by a machine shop. One can cut a larger piece of filter paper to the 55 mm diameter. The second will cut a 55-mm diameter piece, putting the small hole in the center. Besides saving money, these devices (homemade or commercial) allow one
to easily use different types of filter paper. And, I don’t have to buy 55-mm size first.
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<div class="">David</div>
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David M. Belnap<br class="">
Electron Microscopy Core Laboratory<br class="">
Department of Biochemistry<br class="">
School of Biological Sciences<br class="">
2501 SMBB<br class="">
University of Utah<br class="">
Salt Lake City, Utah 84112 USA<br class="">
Phone 801.585.1242<br class="">
FAX 801.587.3077<br class="">
<a href="mailto:David.Belnap@utah.edu" class="">David.Belnap@utah.edu</a><br class="">
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<div class="">On Feb 10, 2020, at 9:07 AM, SubAngstrom <<a href="mailto:info@subangstrom.com" class="">info@subangstrom.com</a>> wrote:</div>
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<div dir="ltr" class="">Thank you Melissa.
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<div class="">Just to add, the cutting edges are tempered; thus are extremely sharp and will not lose their cutting edge.</div>
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<div class="">-SA Team</div>
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<div dir="ltr" class="gmail_attr">On Mon, Feb 10, 2020 at 11:01 AM Chambers, Melissa G <<a href="mailto:melissa.g.chambers@vanderbilt.edu" class="">melissa.g.chambers@vanderbilt.edu</a>> wrote:<br class="">
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<p class="MsoNormal">We have Subangstrom’s punch. You do have to start with 55mm round filter paper and this punches the hole in the center.<u class=""></u><u class=""></u></p>
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<p class="MsoNormal"><a href="https://subangstrom.com/Accessories/Hole-Punch55mm-20mmID" target="_blank" class="">https://subangstrom.com/Accessories/Hole-Punch55mm-20mmID</a><u class=""></u><u class=""></u></p>
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<p class="MsoNormal">In the past, we had an on campus machine shop make one but it wasn’t sharp enough to cut cleanly or easily.<u class=""></u><u class=""></u></p>
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<p class="MsoNormal">Melissa<u class=""></u><u class=""></u></p>
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<p class="MsoNormal"><span style="font-size:10pt;color:rgb(59,56,56)" class="">-----</span><span style="" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Melissa G. Chambers</span><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class=""> </span><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Director, Vanderbilt Cryo-EM Facility</span><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Center for Structural Biology<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Vanderbilt University<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">RR1207 MCN<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">1161 21<sup class="">st</sup> Ave S<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Nashville TN 37232<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">615-322-4671<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif" class=""><a href="http://www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy" target="_blank" class="">www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy</a><u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><b class=""><span style="font-size: 12pt;" class="">From: </span>
</b><span style="font-size: 12pt;" class="">3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank" class="">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Paolo Swuec <<a href="mailto:paolo.swuec@gmail.com" target="_blank" class="">paolo.swuec@gmail.com</a>><br class="">
<b class="">Date: </b>Monday, February 10, 2020 at 9:53 AM<br class="">
<b class="">To: </b>"<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" class="">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" class="">3dem@ncmir.ucsd.edu</a>><br class="">
<b class="">Subject: </b>[3dem] Filter paper cutter/puncher for Vitrobot<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal">Dear list, <u class=""></u><u class=""></u></p>
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<p class="MsoNormal">We've been using commercially available filter paper for our Vitrobot. Given the cost of this consumable (5-10x the cost of Whatman filter paper 595), I am wondering whether anyone can recommend a paper cutter/puncher that can make some
from filter paper sheet. I seem to remember the Gatan CP3 comes with one (if I'm not wrong, long time no see)...<u class=""></u><u class=""></u></p>
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<p class="MsoNormal">Any advice?<u class=""></u><u class=""></u></p>
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<p class="MsoNormal">Best,<u class=""></u><u class=""></u></p>
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<p class="MsoNormal">Paolo <u class=""></u><u class=""></u></p>
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<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">------------------------------------------------</span><u class=""></u><u class=""></u></p>
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<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Paolo Swuec, PhD<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Cryo-Electron Microscopy Lab
</span><span style="font-family:"Segoe UI Symbol",sans-serif;color:rgb(96,96,96)" class="">❆</span><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class=""> Facility Manager<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Dep. BioSciences<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">University of Milano<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Via Celoria 26 - 20133 (Italy)<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Tel. +39 02 503 147 78<u class=""></u><u class=""></u></span></p>
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<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Follow on twitter @PS_CryoEM<u class=""></u><u class=""></u></span></p>
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