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<p class="MsoNormal"><span style="font-size:11.0pt">Hi Matthias, <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Thanks for the comment. I am sending a picture of our setup since several others asked. It’s really convenient most of the time.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">We seem to have problems only occasionally, and with one tank at a time. We noticed that if a tank’s pressure is read too low when hooked up to the system (even if the tank is brand new), the problem is independent
of the side of the manifold and independent of the status of the other tank. For example, if the tank on the left side reads fine and the tank on the right too low (usually reads ~10 psi instead of 22 psi), unfortunately the problem follows the tank. If we
swap them, the “good tank” on the left still reads fine when moved to the right and the 10psi tank still reads 10 psi even when now it’s on the left side.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">It seems with our system, a partially filled tank does not provide enough pressure for the other side to work if the tank reads too low a pressure. But, we will try venting the tanks that are reading 10psi
to see if over-filling is indeed the issue (that would be a very easy fix!).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Again, thank you everyone for the suggestions. Super helpful!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Kristin <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="color:black">From: </span></b><span style="color:black">Matthias Wolf <matthias.wolf@oist.jp><br>
<b>Date: </b>Wednesday, January 29, 2020 at 8:53 AM<br>
<b>To: </b>"Parent, Kristin" <kparent@msu.edu>, 3dem <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] issues with liquid nitrogen tanks<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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<p class="MsoNormal">Hi Kristin,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I doubt you have crappy tanks. Your supplier probably occasionally overfills them. When a tank is filled all the way to the top, there is no gaseous N2 to push out the liquid. In that case simply open the valve opposite of the LN2 valve
for a limited time to generate pressure. Once there is sufficient volume of N2 on top, it becomes self-propelling. <o:p></o:p></p>
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<p class="MsoNormal">It may help with your setup, if you swap only one of the two bottles at a time, so that the half filled one provides enough pressure.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Matthias<o:p></o:p></p>
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<p class="MsoNormal"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Parent, Kristin <kparent@msu.edu><br>
<b>Sent:</b> Wednesday, January 29, 2020 07:21<br>
<b>To:</b> 3dem<br>
<b>Subject:</b> Re: [3dem] issues with liquid nitrogen tanks <o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Thank you everyone for the great suggestions!!! We have some things to try here and I hope it will get resolved easily.
</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Best, </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Kristin </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="color:black">From: </span></b><span style="color:black">3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of "Parent, Kristin" <kparent@msu.edu><br>
<b>Date: </b>Tuesday, January 28, 2020 at 2:45 PM<br>
<b>To: </b>3dem <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[3dem] issues with liquid nitrogen tanks</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt">Hello fellow 3dem-ers,</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">We are experiencing quite an annoying situation with Airgas and I am wondering if other folks have had similar problems and what potential solutions are.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">We have a custom manifold from Chart to deliver liquid nitrogen to our Talos Arctica that can be equipped with two 240L tanks, each with 22 psi pressure. This should help reduce the LN2 waste and be more efficient,
and when our tanks are good, it really is amazing. BUT, some of the time Airgas gives us tanks that we cannot use as the pressure never builds beyond 10 psi and we have to return full tanks. They are not the nicest about this either
</span><span style="font-size:11.0pt;font-family:"Apple Color Emoji"">☹</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">We know the manifold is working correctly, and that it is caused by individual bad tanks. Has anyone else had crappy tanks? Do people just buy their own tanks and have them refilled instead of swapped?</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Unfortunately, in Michigan Airgas is our only option, so any help would be appreciated.
</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Thanks in advance, </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Kristin </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt"> </span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:11.0pt">-- </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Kristin N. Parent, PhD</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">JK Billman, Jr., MD Endowed Research Professor</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Director, MSU Cryo-EM</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:9.0pt;color:black">Associate Professor of Biochemistry and </span><span style="font-size:8.0pt;color:black">Microbiology</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:8.0pt;color:black">Michigan State University</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:8.0pt;color:black">603 Wilson Rd</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:8.0pt;color:black">East Lansing, MI 48824</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:8.0pt;color:black">Phone: 517-432-8434</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:black"><a href="https://kparentlab.natsci.msu.edu/"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:#954F72">https://kparentlab.natsci.msu.edu/</span></a></span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-size:8.0pt;font-family:"Arial",sans-serif;color:black">Twitter: @Phage4Lyfe</span><o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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