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<p><span style="font-size: 12pt;">Hello,</span></p>
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<p>If the PDB <span style="font-size:12pt">of the </span><span style="font-size:12pt">structu</span><span style="font-size:12pt">re has</span><span style="font-size:12pt"> icosahedral 532</span><span style="font-size:12pt"> </span><span style="font-size:12pt">symmetry
which </span><span style="font-size:12pt">symbol (I n=1,2,3,4) should </span><span style="font-size:12pt">i</span><span style="font-size:12pt">n 3D classification and auto</span><span style="font-size:12pt"> refinement? I tried I1 and I4 which gave output while other
indices gave errrors. </span></p>
<p><span style="font-size:12pt">If the particles are 100,000; what is the advisable </span><span style="font-size:12pt">number of 3D classes, what is effect of </span><span style="font-size:12pt">the option "</span><span style="font-size:12pt">-</span><span style="font-size:12pt">-maxsig
300 ". </span></p>
<p><span style="font-size:12pt">Does selecting to "Ignore CTFs until first peak" make a difference in 3D classification.</span></p>
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<p><span style="font-size:12pt">Thank you very much for your help and advice.</span></p>
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