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<p class="MsoNormal">Dear Terje,<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We have an identical Elsa. It had the same clip spring problem when it arrived, but the problem was fixed by Gatan under warranty. Since then, we have collected several datasets using this holder on our Talos 200 with a Falcon3. The best
ones resulted in reconstructions with a 3.7-3.9 A resolution. We did see strong drift once but I blame it on the fact that the holder had been vented due to a power outage shortly before data collection. We will need to collect more datasets to verify this
explanation. As it looks now, this product may have some flaws but it is capable of producing high-quality data.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">I hope this helps.<o:p></o:p></p>
<p class="MsoNormal">Yaroslav<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Yaroslav Tsybovsky, Ph.D. (contractor)<o:p></o:p></p>
<p class="MsoNormal">Scientist II<o:p></o:p></p>
<p class="MsoNormal">Frederick National Laboratory for Cancer Research<o:p></o:p></p>
<p class="MsoNormal" style="mso-line-height-alt:10.05pt;text-autospace:none"><span style="color:black">Leidos Biomedical Research, Inc.
<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-line-height-alt:10.05pt;text-autospace:none"><span style="color:black">Post Office Box B
<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-line-height-alt:10.05pt;text-autospace:none"><span style="color:black">Frederick, Maryland 21702
<o:p></o:p></span></p>
<p class="MsoNormal" style="mso-line-height-alt:10.05pt;text-autospace:none"><span style="color:black">Phone: 301-846-7550
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:black">Yaroslav.Tsybovsky@nih.gov</span><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>From:</b> Dokland, Terje <dokland@uab.edu> <br>
<b>Sent:</b> Thursday, September 5, 2019 12:28 PM<br>
<b>To:</b> 3DEM mailing list <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [3dem] Gatan Elsa holder problems<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Has anybody else had any experience with the Gatan Elsa cryo-holder? We received ours in March and have not been able to use it. It originally arrived with a defective clip spring that was unable to hold the clip ring. It was sent back
for repair,but returned with the same problem. It was sent back again, and upon return it worked for a total of two transfers before the clip spring failed AGAIN. (This is the “standard” tip with the "old fashioned” ring that screws into the tool that’s been
used since the original Gatan holders.) <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Perhaps even more disturbingly, for the two grids that we managed to image before the holder failed, there was a considerable amount of vibration in the x direction (perpendicular to the rod) with greatly attenuated Thon rings, even after
settling for a long time. We have never had this problem with our 626 holder. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">While the extended nitrogen holding time of the Elsa holder would have been advantageous, it seems that this product is so flawed as to be useless. Or have we just been unlucky? Has other people had similar experience? No word from Gatan
yet regarding a replacement, but I think we at least will stick with the old 626 holder from now on. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Terje<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">---<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">Terje Dokland, PhD<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">Professor<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">Department of Microbiology<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">University of Alabama at Birmingham<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">845 19th St South, BBRB 311<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">Birmingham, AL 35294<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">Tel:<span class="apple-style-span"><o:p></o:p></span></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">(205) 996 4502<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">Fax:<span class="apple-style-span"><o:p></o:p></span></span></p>
<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black">(205) 996 2667<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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