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Hi all,
<div>I have a weird behaviour in particle distribution. It is my usual protein (Plasmodium proteasome, C2) but this time I solve it using cryosparc, imported the particles coordinate in Relion, re-extracted and did a bit more cleaning in 2D classes, recreate
a de novo model, 3D classification and refine3D. The distribution plot looks very weird with what looks like a dot matrix around the sphere, I have never seen it before). I have an excess of one orientation but this is not the issue. See pict attached.</div>
<div>Thoughts?</div>
<div>Eric</div>
<div><img src="cid:B61BEE65-5B50-43EC-A490-E9458F6F5F7E" alt="image1.png" id="B61BEE65-5B50-43EC-A490-E9458F6F5F7E"><br>
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<div id="AppleMailSignature" dir="ltr">Assoc. Prof Eric Hanssen
<div><b style="font-size: 13pt; background-color: rgba(255, 255, 255, 0);">Head - Advanced Microscopy Facility</b></div>
<div><b style="font-size: 13pt; background-color: rgba(255, 255, 255, 0);">Honorary Principal Fellow – Department of Biochemistry and Molecular Biology</b></div>
<div><b style="font-size: 13pt; background-color: rgba(255, 255, 255, 0);"><br>
</b></div>
<div><b style="background-color: rgba(255, 255, 255, 0);">President Australian Microscopy and Microanalysis Society</b></div>
<div><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;"><br>
</span></div>
<div><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;">Bio21 Molecular Science and Biotechnology Institute - </span><span style="font-size: 13pt; background-color: rgba(255, 255, 255, 0);">30 Flemington Road - The University of Melbourne
- Victoria 3010 - Australia</span></div>
<div><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;">email:</span><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;"> </span><a href="mailto:ehanssen@unimelb.edu.au" style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;">ehanssen@unimelb.edu.au</a><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;"> </span><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;">|
Office: +61 3 83442449 | Microscope: +61 3 83442509</span><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;"> </span></div>
<div><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;">Web:</span><span style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;"> </span><a href="http://www.microscopy.unimelb.edu.au/" style="background-color: rgba(255, 255, 255, 0); font-size: 13pt;">www.microscopy.unimelb.edu.au</a></div>
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