<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto">Hi, Anchi<div><br></div><div>Cool explanation!</div><div><br></div><div>And here’s a question to your comment.</div><div><br></div><div>How the borderline; 85% nyquist has been determined and what limits that 15%?<br>DQE or some other limitations in detector?</div><div>And the value 15% is empirical or theoretical?</div><div><br></div><div><br></div><div>Best regards,</div><div>Han</div><div><br><br><div id="AppleMailSignature" dir="ltr"><div class="" style="orphans: auto; widows: auto;"><div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; orphans: 2; widows: 2;"><div class=""><b class="" style="background-color: rgba(255, 255, 255, 0);">Bumhan Ryu</b></div><div class=""><span style="background-color: rgba(255, 255, 255, 0);"><br class=""></span></div><div class=""><span style="background-color: rgba(255, 255, 255, 0);">Postdoctoral Researcher</span></div><div class=""><span style="background-color: rgba(255, 255, 255, 0);">Korea Basic Science Institute (KBSI)</span></div><div class=""><div class="" style="orphans: auto; widows: auto;"><span style="background-color: rgba(255, 255, 255, 0);">161 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si</span></div><div class="" style="orphans: auto; widows: auto;"><span style="background-color: rgba(255, 255, 255, 0);">Chungcheongbuk-do, Republic of Korea</span></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div><div class=""><span style="background-color: rgba(255, 255, 255, 0);"><br class=""></span></div><span style="background-color: rgba(255, 255, 255, 0);">Office) <a href="tel:043-240-5329" dir="ltr" x-apple-data-detectors="true" x-apple-data-detectors-type="telephone" x-apple-data-detectors-result="1">043-240-5329</a></span><div class=""><span style="background-color: rgba(255, 255, 255, 0);"><br class="Apple-interchange-newline"><br></span></div></div></div><div dir="ltr"><br>2019. 6. 28. 오전 12:24, Anchi Cheng <<a href="mailto:acheng@nysbc.org">acheng@nysbc.org</a>> 작성:<br><br></div><blockquote type="cite"><div dir="ltr"><span>Hi, Mohamed,</span><br><span></span><br><span>Just want to answer the first part of your first question.</span><br><span></span><br><span>Even though the signal you get in super-resolution mode is real, it is very dampened by DQE</span><br><span>of the detector. Let’s say you have a signal count of 100 above the background across all frequency.</span><br><span>If DQE at your detector pixel limit is 0.2, and at 0.8 at zero frequency. The output of that signal to</span><br><span>your image becomes 100*0.2 = 20 in the former and 80 in the latter. This only gets worse as</span><br><span>you go into super-resolution frequency.</span><br><span></span><br><span>Since protein or other biomolecule do not give strong signal at high resolution to start with,</span><br><span>my “signal across all frequency” assumption above is already false. You can see that information</span><br><span>get through to your image at high frequency is much lower in reality.</span><br><span></span><br><span>If your 1Å pixel data does not give reconstruction resolution to 85% Nyquist resolution (2.35 Å resolution</span><br><span>for 1 Å pixel) with all the modern single-particle processing tricks, the problem is in the specimen. The very</span><br><span>dampened signal in the super resolution range by unbinning your images is not going to help you.</span><br><span></span><br><blockquote type="cite"><span>1- Will my resolution improve if I do not apply bining? </span><br></blockquote><span></span><br><span>Best,</span><br><span></span><br><span>Anchi Cheng</span><br><span>NYSBC</span><br><span>_______________________________________________</span><br><span>3dem mailing list</span><br><span><a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a></span><br><span><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a></span><br></div></blockquote></div></body></html>