<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto">Hi,<div><br></div><div>Please check the followings.</div><div><br></div><div>1. Lens normalization options. Allow Krios to normalize lens right before exposure. There are many options regarding normalization Note that too much robust(frequent) normallization may slow down the acquisition speed.</div><div><br></div><div>2. Check illumination area (size). If the size is too much tight to FOV and the alignment is not that robust, then the thing you mentioned can happen.</div><div><br></div><div>3. Check direct alignment right before data collection and fix it repetitively. Especially, it is suspicious how “condenser center TEM”(c2-c3) is aligned. </div><div><br></div><div>HTH</div><div><br></div><div>Han<br><br><div id="AppleMailSignature" dir="ltr"><div class="" style="orphans: auto; widows: auto;"><div dir="auto" class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;"><div class="" style="font-variant-ligatures: normal; font-variant-position: normal; font-variant-numeric: normal; font-variant-alternates: normal; font-variant-east-asian: normal; line-height: normal; orphans: 2; widows: 2;"><div class=""><b class="" style="background-color: rgba(255, 255, 255, 0);">Bum Han Ryu</b></div><div class=""><span style="background-color: rgba(255, 255, 255, 0);"><br class=""></span></div><div class=""><span style="background-color: rgba(255, 255, 255, 0);">Postdoctoral Researcher</span></div><div class=""><span style="background-color: rgba(255, 255, 255, 0);">Korea Basic Science Institute (KBSI)</span></div><div class=""><div class="" style="orphans: auto; widows: auto;"><span style="background-color: rgba(255, 255, 255, 0);">161 Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-si</span></div><div class="" style="orphans: auto; widows: auto;"><span style="background-color: rgba(255, 255, 255, 0);">Chungcheongbuk-do, Republic of Korea</span></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div><div class=""><span style="background-color: rgba(255, 255, 255, 0);"><br class=""></span></div><span style="background-color: rgba(255, 255, 255, 0);">Office) <a href="tel:043-240-5329" dir="ltr" x-apple-data-detectors="true" x-apple-data-detectors-type="telephone" x-apple-data-detectors-result="1">043-240-5329</a></span><div class=""><span style="background-color: rgba(255, 255, 255, 0);"><br class="Apple-interchange-newline"><br></span></div></div></div><div dir="ltr"><br>2019. 6. 28. 오전 7:36, Claire Atkinson <<a href="mailto:claire.elizabeth.atkinson@gmail.com">claire.elizabeth.atkinson@gmail.com</a>> 작성:<br><br></div><blockquote type="cite"><div dir="ltr"><div dir="ltr">Hi all,<div><br></div><div>We have a Krios set up with a Falcon III camera and after the microscope having run near seamlessly for the last year and a half we've recently been experiencing a new issue when collecting with EPU (v1.9.1). After aligning the microscope and setting everything up we start the run, then within ~15 holes or so the beam will move off axis, possibly a couple of times. If I manually move it back, the rest of the run continues fine. Has anyone else experienced this? Any insight would be appreciated :)</div><div><br></div><div>Best,</div><div><br></div><div>Claire</div></div>
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