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<p>If you would please advise on the following:<br>
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<p>First Case:</p>
<p>The image stacks were acquired in super resolution mode with <span style="font-size:12pt">pixe</span><span style="font-size:12pt">l siz</span><span style="font-size:12pt">e = </span><span style="font-size:12pt">0.53 A. By applying </span><span style="font-size:12pt">bining
during MotionCor the pixel = </span><span style="font-size:12pt">1.06 A . My particle is ~ 150 A and I used box size of 330 pixels = 349.8 A. The calculated defocusing values are below 20,000.</span><span style="font-size:12pt"> The resolution is slightly better
than 3 A.</span></p>
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<p><span style="font-size:12pt">I want to improve the resolution:</span></p>
<p><span style="font-size:12pt">1- Will my resolution improve if I do not apply bining? The new box size will be 660 pixels, is this acceptable and how much RAM and memory on the GPU will it require? (I use 4 GPUs 12GB each).</span></p>
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</span></p>
<p><span style="font-size:12pt">Second Case:</span></p>
<p><span style="font-size:12pt">2-At higher magnification, the pixel size is 0.32 A. To maintanin the same box size and without applying bining, the box size will be 1093 pixels, is this feasible? or I must apply bining?</span></p>
<p>If I apply bining in Second case will the resolution be better than or similar to NOT applying bining in the First case when the pixel size=0.53 A?<br>
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<p><span style="font-size:12pt">3- What b-factor value you apply for super resolution images?</span></p>
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<p><span style="font-size:12pt">Thank you very much for your help and time. </span></p>
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