<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div><blockquote type="cite" class=""><div dir="ltr" class=""><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><br class=""><div class="">The one I have in my possession now did not cool down to 4C for nearly 3 hrs,</div></div></div></div></div></div></blockquote><div><br class=""></div><div>We have now been using the EM GP for several years. At 4 °C, we faced problems of ice cristallisation blocking the trap and arm systems. It was « solved » by Leica engineers by increasing the lower temperature that the device can reach (I don’t remember, between 5 and 7 °C). This setting can be modified in the service menu if you have the code. One way to overcome this problem if it happens is to increase the temperature for a short time so that the ice melts and then come back to 4 °C (after removing water…). Don’t let the water accumulate and remove it periodically.</div><div><br class=""></div><blockquote type="cite" class=""><div class=""><div dir="ltr" class=""><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div class=""> and the humidity was going up and down.</div></div></div></div></div></div></div></blockquote><div><br class=""></div><div>Same problem from time to time … One way we overcome this problem is to unscrew the water tank so that air can circulate more freely inside the device. </div><br class=""><blockquote type="cite" class=""><div class=""><div dir="ltr" class=""><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div class="">Is it the normal behaviour? </div></div></div></div></div></div></div></blockquote><div><br class=""></div><div>I won’t say « normal », but yes it can behaves like that :-(</div><br class=""><blockquote type="cite" class=""><div class=""><div dir="ltr" class=""><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div class="">Also, what is the good regime for protein solutions blotting? Could it be compared to Vitrobot? </div></div></div></div></div></div></div></blockquote><div><br class=""></div><div>No comparison, I never used a Vitrobot. Nevertheless, what’s very cool with the Leica is the temperature control of the liquid ethane. We set it to -184 °C and it can last hours at this temperature without refilling.</div><div><br class=""></div><div>Hope that helps, waiting also for the feedback of other users,</div><div><br class=""></div><div>Denis</div><br class=""><blockquote type="cite" class=""><div class=""><div dir="ltr" class=""><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr" class=""><div dir="ltr" class=""><div dir="ltr" class=""><div class="">Thank you!</div><div class="">Olga</div><div dir="ltr" class=""><br class=""></div><div dir="ltr" class=""><br class="">=======<br class="">Yours sincerely,<br class="">Olga Sokolova, PhD, D.Sc.<br class="">Professor RAS, <span style="font-size:12.8px" class="">Faculty of biology </span><span style="font-size:12.8px" class="">Moscow State University</span></div><div dir="ltr" class="">phone +7-495-938-0005<br class="">cell +7-916-4841251<br class=""><a href="http://proteom-msu.org/en/index.html" target="_blank" class="">http://proteom-msu.org/en/index.html<br class=""></a></div></div></div></div></div></div>
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