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Olga,
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<div class="">I wonder if anyone has a good experience with Leica cryo-plunger for single particle EM applications.</div>
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half my users have been pretty happy with our GP2 plunge freezer, but for the other half the delay between blotting and actually plunging (even when set to auto-plunge) is too long. it’s only 1-2 seconds, but that is long enough for some samples to fall apart.
we have also had some intermittent issues with the blotting paper not rotating properly after blotting. if you are not paying attention and looking for this, you may not even notice. i would advise putting a pen mark on the filter paper so it’s pretty obvious.
the Leica engineer looked at this for us, and of course it worked without fail while he was here so he couldn’t find anything to fix. <br class="">
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<div class="">The one I have in my possession now did not cool down to 4C for nearly 3 hrs, and the humidity was going up and down.</div>
<div class="">Is it the normal behaviour? </div>
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<div>we had this exact same problem with a unit still under warranty. Leica sent an engineer and he replaced the peltier coolers…it now cools down to 4º in 20-30 minutes. if that did not solve the problem, he was going to try replacing the control board for
the unit next. i suggest you get new peltiers.</div>
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<div class="">Also, what is the good regime for protein solutions blotting? Could it be compared to Vitrobot? </div>
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<div>i have found that this is comparable to the settings i used on our old vitrobot. in general i would say that i blot 1-2 seconds longer than i did on the vitrobot for any given sample. i think the GP2 chamber maintains humidity better and therefore needs
longer blot times (but it could also be the fact that it’s only blotting on one side).</div>
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<div>cheers,</div>
<div>Garry</div>
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<div class="">Thank you!</div>
<div class="">Olga</div>
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=======<br class="">
Yours sincerely,<br class="">
Olga Sokolova, PhD, D.Sc.<br class="">
Professor RAS, <span style="font-size:12.8px" class="">Faculty of biology </span><span style="font-size:12.8px" class="">Moscow State University</span></div>
<div dir="ltr" class="">phone +7-495-938-0005<br class="">
cell +7-916-4841251<br class="">
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