<div dir="ltr"><div dir="ltr"><div class="gmail_default" style="font-size:small">Hi Philip,</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small">The mean inner potential (MIP) refers to a total "interaction" potential that is considered a material property. It consists of all the sources contributing to the potential well seen by an imaging electron, including those you suggest (nuclear and electronic contributions.)</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small">Yes, physical changes to the surface via adsorbed matter will directly affect the MIP. I believe the working hypothesis for the source of the "Volta" potential is through heat/exposure related modification of surface adsorbates.</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small">It is also interesting to note that in addition to the electronic character of the object, the surface contributions of adsorbates and heating, there is another thickness independent phase shift (at least for carbon) the source of which I am not clear on. Happy to hear an explanation from anyone in the know : )</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small">Please have a look at this paper where all of the non-Volta contributions are discussed and also measured.</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small"><b>"Electron holography of thin amorphous carbon films: Measurement of the mean inner potential and a thickness-independent phase shift"</b></div><div class="gmail_default" style="font-size:small"><b><br></b></div><div class="gmail_default" style="font-size:small"><b>doi: j.ultramic.2005.10.004</b></div><div class="gmail_default" style="font-size:small"><b><br></b></div><div class="gmail_default" style="font-size:small">HTH</div><div class="gmail_default" style="font-size:small"><br></div><div class="gmail_default" style="font-size:small">Ben</div><div class="gmail_default" style="font-size:small"><b></b><br clear="all"></div><div><div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div><div dir="ltr"><div dir="ltr"><div dir="ltr">------------------------<br><font size="2" face="Tahoma" color="black"><span style="font-size:10pt" dir="ltr"><font size="1"><span style="font-size:13px"><font face="Arial">Benjamin Himes</font><font face="Arial"><br>
</font><font face="Arial"><br>
cryoEM methods development </font><font face="Arial"><br>
</font><a href="https://mail.hhmi.org/owa/redir.aspx?C=SbsCefkcbOt75jDyr05lpd3OifVN_utmfvnhZrtXS7Bl2i2eOXXVCA..&URL=http%3a%2f%2fgrigoriefflab.janelia.org%2f" target="_blank"><font face="Arial">Grigorieff lab</font></a><font face="Arial">, HHMI Janelia Research Campus</font><font face="Arial"><br>
</font><font face="Arial"><br>
cryoSTAC development @ </font><a href="https://mail.hhmi.org/owa/redir.aspx?C=8yDzXj54yTidMevTB7q5m3liEVwqAZ9LxuXQ4iYOVvtl2i2eOXXVCA..&URL=https%3a%2f%2fgithub.com%2fbHimes%2femClarity%2fwiki" target="_blank"><font face="Arial">emClarity</font></a><font face="Arial"><br><br>-------------------------</font></span></font></span></font></div></div></div></div></div></div></div></div></div></div></div><br></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Mon, Jun 17, 2019 at 12:17 PM <<a href="mailto:3dem-request@ncmir.ucsd.edu">3dem-request@ncmir.ucsd.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Send 3dem mailing list submissions to<br>
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Today's Topics:<br>
<br>
1. mean inner potential of a solid (Philip K?ck)<br>
2. NYC Computational Cryo-EM Summer Workshop<br>
(Cindy Rampersad-Phillips)<br>
3. side entry holder for autogrids? (Michael Elbaum)<br>
4. Re: side entry holder for autogrids? (Wim Hagen)<br>
<br>
<br>
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<br>
Message: 1<br>
Date: Mon, 17 Jun 2019 08:27:35 +0000<br>
From: Philip K?ck <<a href="mailto:philip.koeck@ki.se" target="_blank">philip.koeck@ki.se</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>>,<br>
"<a href="mailto:microscopy@microscopy.com" target="_blank">microscopy@microscopy.com</a>" <<a href="mailto:microscopy@microscopy.com" target="_blank">microscopy@microscopy.com</a>><br>
Subject: [3dem] mean inner potential of a solid<br>
Message-ID:<br>
<<a href="mailto:HE1PR0802MB2586733E695E56B20FBCC6D183EB0@HE1PR0802MB2586.eurprd08.prod.outlook.com" target="_blank">HE1PR0802MB2586733E695E56B20FBCC6D183EB0@HE1PR0802MB2586.eurprd08.prod.outlook.com</a>><br>
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<br>
Hi all.<br>
<br>
I've been wondering what the mean inner electrostatic potential of a solid (for example the 10 V of carbon) is actually due to.<br>
Is it purely caused by the distribution of nuclei and electrons in the solid itself or could there be a contribution from adsorbed surface charges?<br>
<br>
All the best,<br>
<br>
Philip<br>
<br>
<br>
<br>
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Message: 2<br>
Date: Mon, 17 Jun 2019 10:46:34 -0400<br>
From: Cindy Rampersad-Phillips <<a href="mailto:crampersad@flatironinstitute.org" target="_blank">crampersad@flatironinstitute.org</a>><br>
To: <a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a><br>
Subject: [3dem] NYC Computational Cryo-EM Summer Workshop<br>
Message-ID:<br>
<<a href="mailto:CAP2feS4-oznv2pinFh_mVdu403f1sU-XNQ1iTRr0mS9nyzk82Q@mail.gmail.com" target="_blank">CAP2feS4-oznv2pinFh_mVdu403f1sU-XNQ1iTRr0mS9nyzk82Q@mail.gmail.com</a>><br>
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<br>
Dear 3D-EM group,<br>
<br>
We are organizing a 2-day workshop on "Computational Cryo-EM" which will<br>
take place at the Flatiron Institute, Simons Foundation, in New York City,<br>
on August 8-9, 2019. This summer workshop revives a tradition started by<br>
Joachim Frank and Gabor Herman many years ago. The topics will include:<br>
advances in reconstruction algorithms, software implementations and<br>
automation, motion correction, 2D/3D classification and continuous<br>
heterogeneity analysis, and related mathematical results and new problems.<br>
There will be 16 speakers in total, each giving 30-minute talks, in a<br>
workshop setting which promotes the open exchange of ideas. In addition,<br>
there will be a poster session.<br>
<br>
For more information, including the list of confirmed speakers, see the<br>
event website:<br>
<br>
<a href="https://www.simonsfoundation.org/event/nyc-computational-cryo-em-summer-workshop/" rel="noreferrer" target="_blank">https://www.simonsfoundation.org/event/nyc-computational-cryo-em-summer-workshop/</a><br>
<br>
To apply, please fill in the following registration form:<br>
<br>
<a href="https://indico.flatironinstitute.org/e/CryoEM" rel="noreferrer" target="_blank">https://indico.flatironinstitute.org/e/CryoEM</a><br>
<br>
Registration will be closed once we reach capacity, so please register soon<br>
to guarantee your attendance.<br>
<br>
On behalf of the organizing committee,<br>
<br>
Joakim And?n (Flatiron)<br>
Alex Barnett (Flatiron)<br>
Leslie Greengard (Flatiron / NYU)<br>
Roy Lederman (Yale)<br>
Amit Singer (Princeton)<br>
Marina Spivak (Flatiron)<br>
<br>
Regards,<br>
Cindy<br>
-- <br>
Cindy Rampersad-Phillips<br>
Coordinator, Center for Computational Mathematics<br>
<br>
*Flatiron Institute*<br>
162 Fifth Ave<br>
<<a href="https://www.google.com/maps/place/Flatiron+Institute/@40.7405662,-73.9907621,15z/data=!4m5!3m4!1s0x0:0x8849799a20fe5d4a!8m2!3d40.7405662!4d-73.9907621" rel="noreferrer" target="_blank">https://www.google.com/maps/place/Flatiron+Institute/@40.7405662,-73.9907621,15z/data=!4m5!3m4!1s0x0:0x8849799a20fe5d4a!8m2!3d40.7405662!4d-73.9907621</a>><br>
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646.603.3750 *main*<br>
646.603.3718 *direct *<br>
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Message: 3<br>
Date: Mon, 17 Jun 2019 16:00:45 +0000<br>
From: Michael Elbaum <<a href="mailto:michael.elbaum@weizmann.ac.il" target="_blank">michael.elbaum@weizmann.ac.il</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: [3dem] side entry holder for autogrids?<br>
Message-ID: <77389ED5A5E5A947A5FEA1B9E8D512CB01961EB25D@ibwmbx03><br>
Content-Type: text/plain; charset="iso-8859-1"<br>
<br>
Hi all, wondering if anyone has heard/thought/dreamed of a side entry holder for clipped grids? This would be very useful in mixed company (of microscopes, of course), and could enable analysis of grids post-tomography by non-standard methods such as EDS. Perhaps someone has experience in modifying a 626 tip for the purpose?<br>
regards,<br>
Michael<br>
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Message: 4<br>
Date: Mon, 17 Jun 2019 18:16:32 +0200<br>
From: Wim Hagen <<a href="mailto:hagen@embl.de" target="_blank">hagen@embl.de</a>><br>
To: "<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
Subject: Re: [3dem] side entry holder for autogrids?<br>
Message-ID: <<a href="mailto:44A00032-E921-429F-8BD0-03AA24FEC02A@embl.de" target="_blank">44A00032-E921-429F-8BD0-03AA24FEC02A@embl.de</a>><br>
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Hi Michael,<br>
<br>
I?ve discussed this with Chris Booth from Gatan some time ago, a multiple cartridge side entry holder will for sure also help a lot of people with side-entry screening systems, preparing for later data collection in central facilities. With other people?s ideas for cheap side-entry low-kv screening tools, this could be quite a market.<br>
<br>
Best,<br>
<br>
Wim Hagen<br>
EMBL Heidelberg<br>
<br>
> On 17. Jun 2019, at 18:00, Michael Elbaum <<a href="mailto:michael.elbaum@weizmann.ac.il" target="_blank">michael.elbaum@weizmann.ac.il</a>> wrote:<br>
> <br>
> Hi all, wondering if anyone has heard/thought/dreamed of a side entry holder for clipped grids? This would be very useful in mixed company (of microscopes, of course), and could enable analysis of grids post-tomography by non-standard methods such as EDS. Perhaps someone has experience in modifying a 626 tip for the purpose?<br>
> regards,<br>
> Michael <br>
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