<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
<style type="text/css" style="display:none"><!-- p { margin-top: 0px; margin-bottom: 0px; }--></style>
</head>
<body dir="ltr" style="font-size:12pt;color:#000000;background-color:#FFFFFF;font-family:Calibri,Arial,Helvetica,sans-serif;">
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">Hello,</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;"><br>
</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">I hope you would please advise on the following:<br>
</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;"><br>
</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">When</span><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);"> I
apply post processing in Relio</span><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">n on a refined 3D map, the FSC curve gives 0.3 Angstrom worse resolution than that before post processing.</span><br>
</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;"><br>
</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">If I use Resmap v 1.95 to estimate the resolution from the unfiltered half maps of the refined model, the resolution is 0.4 A better than when I use the resolution estimation from
Relion on the same unfiltered half map.</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;"><br>
</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">- Which value to believe? <br>
</p>
<p style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;"><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px; background-color: rgb(255, 255, 255);">- How to calculate FSC without post processing ?</span><br>
</p>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">
- What caused the post processing to give lower resolution and resolution estimation to be lower?</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">
Can you please point out for a source on how to reliably fit a pdb into an cryo map?<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">
Thank you very much for your help and time.</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">
<br>
</div>
<div style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 16px;">
<br>
</div>
<p><br>
</p>
</body>
</html>