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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Dear Anastasia,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">We tried out the ultra-low profile Elsa and liked the ability to recover the grids but did not like the robustness of the clipping system. We have a Gatan 914
holder and have had to replace the front spring on several occasions because it eventually gets bent or loose and no longer clamps the grid properly. This may be why you are seeing with the leopard skin ice. The conduction on the grid may not be sufficient
to maintain a temperature that will keep vitreous ice on the grid. This holder is great for a very experienced and careful user but definitely not for general use. That is our experience with the Elsa. Hope it helps.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Bob<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu]
<b>On Behalf Of </b>Anastasia Gazi<br>
<b>Sent:</b> Wednesday, June 12, 2019 8:01 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] Elsa cryo holder<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Dear all,
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">I would be grateful if someone of you could share some more information related to the ELSA cryoholders.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">We extensively tried out the ultra-low profile (ULP) tip for 2 weeks and the results were quite controversial.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">The stability of the holder was amazing in comparison to our 626 cryoholders but there was a regular appearing of leopard/orange skin on the grids.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">We believe that this might be related to leakage at the loading station but I wonder if someone else had the same experience with its own ELSA (ULP or standard tip profile).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Thank you a lot for your help,
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Anastasia <o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Sat, 6 Jan 2018 at 00:38, David Michael Belnap <<a href="mailto:David.Belnap@utah.edu">David.Belnap@utah.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">I don’t know, Benjamin. Elsa freezes things so fast that maybe she is really making vitreous ice! :-)
<o:p></o:p></p>
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<p class="MsoNormal">On Jan 4, 2018, at 10:13 AM, Benjamin Bammes <<a href="mailto:bbammes@gmail.com" target="_blank">bbammes@gmail.com</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">While I don't know anything about the holder, I find the name "Elsa" a bit humorous. For anyone with young kids who has watched the movie Frozen, you know that Elsa does two things: (1) She turns everything she touches to solid ice, and
(2) She always wants to "let it go." Both of those things are undesirable for cryo holders! :-)<o:p></o:p></p>
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<p class="MsoNormal" style="line-height:16.55pt"><span style="font-size:10.0pt;font-family:"Helvetica",sans-serif">----<br>
<b>Benjamin Bammes</b></span><span style="font-family:"Helvetica",sans-serif"><o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Wed, Jan 3, 2018 at 9:21 PM, jamonshi <<a href="mailto:jamonshi@gmail.com" target="_blank">jamonshi@gmail.com</a>> wrote:<o:p></o:p></p>
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<p>Our local sales says<o:p></o:p></p>
<p>"Gatan has not shipped any production 698 Elas holder yet. The holders won’t start shipping until March of 2018. Gatan cannot discover where they tested the holder, as that is not a complete product and the development is confidential."
<o:p></o:p></p>
<p class="MsoNormal">Cheers,<br>
Jian <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On 04/01/2018 06:36, Elizabeth Montabana wrote:<o:p></o:p></p>
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<p class="MsoNormal">Hi All <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I haven't seen any responses to this, but we are also curious about if anyone has purchased or tested an Elsa Cryo transfer holder and their opinion about it - particularly, if folks have been able to get high resolution data with it. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">thanks<o:p></o:p></p>
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<p class="MsoNormal">Liz Montabana<o:p></o:p></p>
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<p class="MsoNormal">Donner Labs, LBNL<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Wed, Nov 29, 2017 at 4:54 AM, Anastasia Gazi <<a href="mailto:anadegaz@gmail.com" target="_blank">anadegaz@gmail.com</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Hello all,<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><o:p> </o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">We are considering the purchase of an Elsa Cryo transfer holder Model 698 from Gatan. It is looking great on paper with its centrosymmetric design, extended hold time to 8h and
everything but I am wondering if someone has already experience with it. Either with the standard profile holder or with the ultra-low one.
<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><o:p> </o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Thank you for your help,
<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">Anastasia<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><o:p> </o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><o:p> </o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><o:p> </o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">==================================================<br>
Anastasia D. Gazi, PhD.<br>
Ultrastructural BioImaging unit,<br>
Départment de Biologie cellulaire et infection,<br>
Institut Pasteur<br>
28, rue du Dr. Roux<br>
75015 PARIS<br>
FRANCE<o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto"><a href="mailto:agazi@pasteur.fr" target="_blank">agazi@pasteur.fr</a><o:p></o:p></p>
<p class="MsoNormal" style="mso-margin-top-alt:auto;mso-margin-bottom-alt:auto">==================================================<o:p></o:p></p>
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<pre>_______________________________________________<o:p></o:p></pre>
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