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<p class="MsoNormal">We tried 3D printing boxes here a few years ago with mixed success. The standard 3D filament (ABS) made a nice, robust box which would invariably crack once under LN2 and any torque was applied to the closing screw. We tried a couple
different materials, including a squishy rubber (not certain anymore what the material was) that
<b>exploded</b> once immersed in LN2. In the end, there really wasn’t a cost savings.<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Andy<o:p></o:p></p>
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<p class="MsoNormal">-- <o:p></o:p></p>
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<p class="MsoNormal">Andrew Kehr, PhD<o:p></o:p></p>
<p class="MsoNormal">IRTA Fellow<o:p></o:p></p>
<p class="MsoNormal">Structural Cell Biology, LCMB, NIDDK, NIH<o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">Eric Hanssen <ehanssen@unimelb.edu.au><br>
<b>Date: </b>Tuesday, June 11, 2019 at 1:20 AM<br>
<b>To: </b>Davide Zabeo <davide.zabeo@gu.se><br>
<b>Cc: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>Re: [3dem] 3D printed cryo-grid boxes<o:p></o:p></span></p>
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<p class="MsoNormal">Hi Davide, <o:p></o:p></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt">what I do is I bring the empty grid boxes that comes with the grids we purchase (the blue kind) to our workshop and they cut the boxes and lids in little 4 grid squares, add a little screw (Teflon, but my last
batch was metal and they are much better) et voila! Cost $1 per grid box for the labour<o:p></o:p></p>
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<p class="MsoNormal">Assoc. Prof Eric Hanssen <o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:13.0pt">Head - Advanced Microscopy Facility</span></b><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:13.0pt">Honorary Principal Fellow – Department of Biochemistry and Molecular Biology</span></b><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b>President Australian Microscopy and Microanalysis Society</b><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:13.0pt">Bio21 Molecular Science and Biotechnology Institute - 30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia</span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:13.0pt">email: </span><a href="mailto:ehanssen@unimelb.edu.au"><span style="font-size:13.0pt">ehanssen@unimelb.edu.au</span></a><span style="font-size:13.0pt"> | Office: +61 3 83442449 | Microscope: +61 3 83442509 </span><o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:13.0pt">Web: </span><a href="http://www.microscopy.unimelb.edu.au/"><span style="font-size:13.0pt">www.microscopy.unimelb.edu.au</span></a><o:p></o:p></p>
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On 11 Jun 2019, at 12:46, John Crum <<a href="mailto:jcrum12@cox.net">jcrum12@cox.net</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">Davide,<br>
<br>
I have modeled and printed cryo grid boxes. I have not used them, though. I decided not to pursue making and selling them because the cost was about the same as the commercially available ones (about USD 9). I'm not sure that the plastic from any 3D printer
type would stand up to LN2 temperatures, over the long run. I do make and sell a manual plunge freezing workstation. You can see it on the 3D printer service "Shapeways" at:<br>
<br>
<a href="https://www.shapeways.com/product/HZSNAVE4B/plungefreezeinsert-base-v5-1-3">https://www.shapeways.com/product/HZSNAVE4B/plungefreezeinsert-base-v5-1-3</a><br>
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Let me know if you have any questions...<br>
<br>
jc<br>
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John Crum<br>
JC Designs/California<br>
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<p class="MsoNormal" style="margin-bottom:12.0pt">On June 10, 2019 at 2:22 AM Davide Zabeo <<a href="mailto:davide.zabeo@gu.se">davide.zabeo@gu.se</a>> wrote:
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<p><span style="font-size:12.0pt;color:black">Dear all,<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black">In our lab we would like to try 3D-printing cryo-boxes to store plunge frozen cryo-grids (attached photo as reference).<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black">Has anyone tried this before? Does anyone have a blueprint of it?<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black">Any help is appreciated! Thank you.<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
<p><span style="font-size:12.0pt;color:black">Best wishes,<o:p></o:p></span></p>
<p><span style="font-size:12.0pt;color:black"><o:p> </o:p></span></p>
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<p style="background:white"><span class="blocktitle"><b><span style="color:black">Davide Zabeo</span></b></span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p style="background:white"><span style="color:black">PhD student</span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p style="background:white"><span style="color:black">Johanna Höög Lab</span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p style="background:white"><span style="color:black">Department of Chemistry and Molecular Biology</span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
<p style="background:white"><span style="color:black">University of Gothenburg<br>
Visiting Address: <span class="addressvalue">Medicinaregatan 9 C</span>, 41390 Göteborg <br>
Postal Address: <span class="addressvalue">Box 462</span>, 40530 Göteborg SWEDEN</span><span style="font-size:12.0pt;color:black"><o:p></o:p></span></p>
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