<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><br class=""><div><br class=""><blockquote type="cite" class=""><div class="">On Jun 10, 2019, at 2:22 AM, Davide Zabeo <<a href="mailto:davide.zabeo@gu.se" class="">davide.zabeo@gu.se</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div id="divtagdefaultwrapper" dir="ltr" style="font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-size: 12pt; font-family: Calibri, Helvetica, sans-serif;" class=""><p style="margin-top: 0px; margin-bottom: 0px;" class=""></p><div id="divtagdefaultwrapper" dir="ltr" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif, EmojiFont, "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols;" class=""><p style="margin-top: 0px; margin-bottom: 0px;" class=""></p><div id="divtagdefaultwrapper" dir="ltr" style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif, EmojiFont, "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols;" class=""><div style="margin-top: 0px; margin-bottom: 0px;" class="">Dear all,</div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><br class=""></div><div style="margin-top: 0px; margin-bottom: 0px;" class="">In our lab we would like to try 3D-printing cryo-boxes to store plunge frozen cryo-grids (attached photo as reference).</div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><br class=""></div><div style="margin-top: 0px; margin-bottom: 0px;" class="">Has anyone tried this before? Does anyone have a blueprint of it?</div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><br class=""></div><div style="margin-top: 0px; margin-bottom: 0px;" class="">Any help is appreciated! Thank you.</div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><br class=""></div><div style="margin-top: 0px; margin-bottom: 0px;" class="">Best wishes,</div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><br class=""></div><div id="Signature" class=""><div id="divtagdefaultwrapper" dir="ltr" style="font-size: 12pt; background-color: rgb(255, 255, 255); font-family: Calibri, Arial, Helvetica, sans-serif, EmojiFont, "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols;" class=""><div style="margin-top: 0px; margin-bottom: 0px;" class=""><span style="font-size: 11pt;" class=""><span class="blockTitle" style="font-weight: bold; line-height: 18px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 11pt;"><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class="">Davide Zabeo</span></span></span></span></span><span style="line-height: 18px; font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><br class=""></span></span></span></span></span></span></div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><span style="font-size: 11pt;" class=""><span style="line-height: 18px; font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class="">PhD student</span></span></span></span></span></span></div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><span style="font-size: 11pt;" class=""><span style="line-height: 18px; font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class="">Johanna Höög Lab</span></span></span></span></span></span></div><div style="margin-top: 0px; margin-bottom: 0px;" class=""><span style="line-height: 18px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 11pt;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class="">Department of Chemistry and Molecular Biology</span></span></span></span></div><div style="margin-top: 0px; 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font-size: 11pt;" class=""></span><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 11pt;" class=""></span><span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 11pt;" class=""></span><span style="line-height: 18px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 11pt;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class="">Postal Address: </span></span></span></span></span><span class="addressValue" style="text-transform: capitalize; line-height: 18px; font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 11pt;"><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class=""><span style="font-family: Calibri, Arial, Helvetica, sans-serif;" class="">Box 462</span></span></span></span></span><span style="line-height: 18px; 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text-decoration: none; float: none; display: inline !important;" class="">_______________________________________________</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class=""><span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class="">3dem mailing list</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class=""><a href="mailto:3dem@ncmir.ucsd.edu" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">3dem@ncmir.ucsd.edu</a><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class=""><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a></div></blockquote></div><div class=""><br class=""></div>Dear Davide,<div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>I do not have a comment specific to 3-D printing, but I found that the “witches hat” design for the lid has at least two flaws. Fewer specimens can be put into a 50 ml tube, and, more importantly, grids can be pulled out of the base and will then stick to the underside of the lid as it is being unscrewed. The alternative, a flat disk with a U-shaped channel that can be rotated over one of the slots in the base or left so that all the slots are covered, worked much better for me, and I also recommend that metal screws be used to hold the lid to the base. YMMV. As long as one wants to use a 3-D printer, it would be as easy to make one type of lid as another.<br class=""><div class="">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div class=""><span class="Apple-tab-span" style="white-space: pre; "> </span>Yours,</div><div class=""><span class="Apple-tab-span" style="white-space: pre; "> </span>Bill</div><div class=""><br class=""></div></span><br class="Apple-interchange-newline">
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