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Hi Andrei,
<div class="">Our core has a 2100 with LAB6 for screening with a DE-12 and a Gatan cryoholder. It is a very nice screening instrument. Potentially good for perhaps 6-10 Å on cryo specimens if everything is optimal, but we generally move to a better instrument
as soon as we confirm we have good grids.</div>
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<font face="Courier" class=""><span style="font-size: 14px;" class="">--------------------------------------------------------------------------------------<br class="">
Steven Ludtke, Ph.D. <<a href="mailto:sludtke@bcm.edu" class="">sludtke@bcm.edu</a>> Baylor College of Medicine <br class="">
Charles C. Bell Jr., Professor of Structural Biology<br class="">
Dept. of Biochemistry and Molecular Biology (<a href="http://www.bcm.edu/biochem" class="">www.bcm.edu/biochem</a>)<br class="">
Academic Director, CryoEM Core (<a href="http://cryoem.bcm.edu" class="">cryoem.bcm.edu</a>)<br class="">
Co-Director CIBR Center (<a href="http://www.bcm.edu/research/cibr" class="">www.bcm.edu/research/cibr</a>)<br class="">
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<blockquote type="cite" class="">
<div class="">On Jun 7, 2019, at 7:25 PM, Andrei Moiseenko <<a href="mailto:postmoiseenko@gmail.com" class="">postmoiseenko@gmail.com</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class=""><font size="2" style="font-family: Helvetica; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; text-decoration: none;" class=""><b class="">***CAUTION:***
This email is not from a BCM Source. Only click links or open attachments you know are safe.</b></font><span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class=""></span>
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<div class="WordSection1" style="page: WordSection1; caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;">
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Dear all,<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
I consider upgrading my microscope to a cryo-em. My purpose is to use it as a screening device and also for low-resolution single particle data acquisition. But I am not sure if our configuration can possibly fit this purpose, so I’m seeking for an advise.<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
What we already have:<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt 36pt; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: -18pt;" class="">
<span class="">1.<span style="font-style: normal; font-variant-caps: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";" class=""> <span class="Apple-converted-space"> </span></span></span>JEOL
JEM-2100: 200kV, Cs 1.4,<span class="Apple-converted-space"> </span><span lang="RU" class="">Сс</span><span class="Apple-converted-space"> </span>1.8, LaB6 gun<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt 36pt; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: -18pt;" class="">
<span class="">2.<span style="font-style: normal; font-variant-caps: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";" class=""> <span class="Apple-converted-space"> </span></span></span>Anticontaminator:
JEOL cryo-fin (not a twin-blade style).<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt 36pt; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: -18pt;" class="">
<span class="">3.<span style="font-style: normal; font-variant-caps: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";" class=""> <span class="Apple-converted-space"> </span></span></span>Pretty
fine Thon rings up to 5A at -1mkm defocus<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt 36pt; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: -18pt;" class="">
<span class="">4.<span style="font-style: normal; font-variant-caps: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";" class=""> <span class="Apple-converted-space"> </span></span></span>Three
condenser lenses, nice parallel illumination<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt 36pt; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: -18pt;" class="">
<span class="">5.<span style="font-style: normal; font-variant-caps: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";" class=""> <span class="Apple-converted-space"> </span></span></span>Stable
1.0-1.1 eV electron energy spread, measured from EELS ZLP<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt 36pt; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: -18pt;" class="">
<span class="">6.<span style="font-style: normal; font-variant-caps: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";" class=""> <span class="Apple-converted-space"> </span></span></span>Already
set up single particle acquisition with serialEM for negative stained samples.<o:p class=""></o:p></div>
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What we consider to get:<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt 36pt; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: -18pt;" class="">
<span class="">1.<span style="font-style: normal; font-variant-caps: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";" class=""> <span class="Apple-converted-space"> </span></span></span>Direct
Electron DE-20 instead of a 2k CCD<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt 36pt; font-size: 11pt; font-family: Calibri, sans-serif; text-indent: -18pt;" class="">
<span class="">2.<span style="font-style: normal; font-variant-caps: normal; font-weight: normal; font-stretch: normal; font-size: 7pt; line-height: normal; font-family: "Times New Roman";" class=""> <span class="Apple-converted-space"> </span></span></span>Gatan
cryo-transfer holder.<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
I would appreciate any opinion on this upgrade. Does it seem reasonable or not?<o:p class=""></o:p></div>
<div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">
I will also be very grateful for any suggestions about highest possible resolution on such a system and about any important limitations that we should be aware of.<o:p class=""></o:p></div>
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<o:p class=""> </o:p></div>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt; font-size: 11pt; font-family: Calibri, sans-serif; line-height: 16.866666793823242px;">
Best regards,<br class="">
Andrey Moiseenko<o:p class=""></o:p></p>
<p class="MsoNormal" style="margin: 0cm 0cm 10pt; font-size: 11pt; font-family: Calibri, sans-serif; line-height: 16.866666793823242px;">
Electron Microscopy Lab @ Biology Department<br class="">
Moscow State University<br class="">
<a href="mailto:postmoiseenko@gmail.com" style="color: rgb(149, 79, 114); text-decoration: underline;" class=""><span style="color: blue;" class="">postmoiseenko@gmail.com</span></a><span class="Apple-converted-space"> </span><br class="">
skype: callmoiseenko<o:p class=""></o:p></p>
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