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<p>Hello,<br>
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<p>If you would please advise on the following<br>
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<p>In Motion correction, f<span style="font-size:12pt">o</span><span style="font-size:12pt">r
</span><span style="font-size:12pt">super-resolution movies</span><span style="font-size:12pt">:</span></p>
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<p>1-What is the<span style="font-size:12pt"> recommended</span><span style="font-size:12pt"> b-factor</span><span style="font-size:12pt"> and number of patches?</span></p>
<p><span style="font-size:12pt">2-If the plot of early motion is around zero and total motion and late motion are the same (plots are attached), </span></p>
<p><span style="font-size:12pt">what does this mean? how to judge if motion correction is successful? <span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:16px; background-color:rgb(255,255,255)">What is the acceptable total, early and late motion
value range? Sometimes the logfile is not written out only individual eps files?</span></span></p>
<p><span style="font-size:12pt">52 e/A^2, 21 frames,0.2 s each</span></p>
<p><span style="font-size:12pt"></span></p>
<p><span style="font-size:12pt">3-What is the acceptable range for dose per frame? If a lower value than the actual dose is used, does it affect/improve the output?</span></p>
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<p><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)">4- I</span><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)">s there any</span><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)"> extra options
to include </span><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)">in MotionCorr2 for </span><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)">super-resolution
movies</span><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)">?</span><br>
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<p><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)"><br>
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<p><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)">5- GCTF: is there extra options to specify as validation or high resolution? and what is the command line?</span></p>
<p><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)"><br>
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<p><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)">6- Is there an exact mathematical relation between the defocus range and step size that should be used for acquisition and the particle size?</span></p>
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<p><span style="font-family:Calibri,Arial,Helvetica,sans-serif; font-size:12pt; background-color:rgb(255,255,255)">Thank you very much for your help and time.</span></p>
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