<div dir="ltr">I would argue that it all depends on what the goal is, ultimately. Screening cryoEM samples or try to get 3D reconstructions at < 5 A for single particles, or helical assemblies/viruses, etc?<div><br></div><div><br><div><br></div><div><br></div></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Apr 24, 2019 at 11:10 PM Benjamin Bammes <<a href="mailto:bbammes@gmail.com">bbammes@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="auto">It's pretty clear from the progress in cryo-EM over the past decade that direct detectors make a huge impact on data quality. At this point, scintillator-coupled cameras are a major disadvantage for cryo-EM. <div dir="auto"><br></div><div dir="auto">Regarding cost, I'd encourage you to talk to the various manufactors (including our company, Direct Electron). We have two options for lower-cost direct detectors, and it is also sometimes possible to purchase ex-demo cameras at a reduced price.</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Apr 24, 2019, 3:59 PM Denis Chrétien <<a href="mailto:denis.chretien@univ-rennes1.fr" target="_blank">denis.chretien@univ-rennes1.fr</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Dear all,<br>
<br>
We would like to upgrade our 200 kV LaB6 ( Tecnai G2 T20 Sphera FE ) with a a new camera. We currently have an Ultrascan 4K from Gatan and are wondering whether a direct electron camera would drastically improve our images (at quite an expensive price), or if a less expensive camera like the RIO that allows motion correction would be sufficient considering the limited resolution of our microscope. Any feedback on this issue would be greatly appreciated.<br>
<br>
Best,<br>
<br>
Denis<br>
<br>
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