<html><head><meta http-equiv="Content-Type" content="text/html charset=us-ascii"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Ana,<div class=""><br class=""></div><div class="">We have just started using one in the Nogales Lab with the 2 following settings (1 for Quantifoil or C-flats, 2 for regular thick carbon films):</div><div class=""><br class=""></div><div class=""><span class="Apple-tab-span" style="white-space:pre"> </span>Mode<span class="Apple-tab-span" style="white-space:pre"> </span>power<span class="Apple-tab-span" style="white-space:pre"> </span>duty ratio<span class="Apple-tab-span" style="white-space:pre"> </span>gas (sccm) <span class="Apple-tab-span" style="white-space:pre"> </span>duration (m:s)</div><div class="">1 <span class="Apple-tab-span" style="white-space:pre"> </span>remote<span class="Apple-tab-span" style="white-space:pre"> </span>15W<span class="Apple-tab-span" style="white-space:pre"> </span>64<span class="Apple-tab-span" style="white-space:pre"> </span>10.0<span class="Apple-tab-span" style="white-space:pre"> </span>1:00</div><div class="">2<span class="Apple-tab-span" style="white-space:pre"> </span>remote<span class="Apple-tab-span" style="white-space:pre"> </span>15W<span class="Apple-tab-span" style="white-space:pre"> </span>255<span class="Apple-tab-span" style="white-space:pre"> </span>10.0<span class="Apple-tab-span" style="white-space:pre"> </span>0:30<br class=""><div class=""><br class="webkit-block-placeholder"></div><div class="">We have had good results with those settings using air as gas, both for freezing grids (1) or negative stain (2) so far.</div><div class="">Cheers,</div><div class=""><br class=""></div><div class="">Patricia</div><div class=""><br class=""></div><div class="">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">_______________________________<br class=""><font color="#929292" class="">Patricia Grob, Ph.D.<br class="">Research Specialist<br class="">Nogales Lab<br class="">HHMI at UC Berkeley<br class="">URL: <a href="http://cryoem.berkeley.edu" class="">cryoem.berkeley.edu</a><br class="">Email: <a href="mailto:pgrob@berkeley.edu" class="">pgrob@berkeley.edu</a><br class=""><br class=""><br class=""></font><br class=""></div></div>
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<br class=""><div><blockquote type="cite" class=""><div class="">On Mar 27, 2019, at 11:57 AM, Ana B Asenjo <<a href="mailto:ana.asenjo@einstein.yu.edu" class="">ana.asenjo@einstein.yu.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div class="">Hi,<br class=""><br class="">Does anyone have experience using the Tergeo-EM plasma cleaner to glow discharge EM grids (carbon, quantifoil or gold grids). What setting do you use?<br class=""><br class="">Thanks<br class=""><br class="">Ana<br class="">_______________________________________________<br class="">3dem mailing list<br class=""><a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><br class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem<br class=""></div></div></blockquote></div><br class=""></div></body></html>